Glucose isomerase of the Streptomyces sp. SK strain: Purification, sequence analysis and implication of alanine 103 residue in the enzyme thermostability and acidotolerance

Biochimie

Volumn 86, Issue 8, 2004, Pages 561-568

  Borgi, Mohamed Ali ^a   Srih Belguith, Karima ^a   Ben Ali, Mamdouh ^a   Mezghani, Monia ^a   Tranier, Samuel ^b   Haser, Richard ^b   Bejar, Samir ^a  

^a CENTRE DE BIOTECHNOLOGIE DE SFAX   (Tunisia)

^b INSTITUT DE BIOLOGIE ET CHIMIE DES PROTÉINES   (France)

Author keywords

Glucose isomerase; Homology modelling; Purification; Site directed mutagenesis; Thermostability

Indexed keywords

ALANINE; GLUCOSE ISOMERASE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL STRAIN; CRYSTAL STRUCTURE; ENZYME PURIFICATION; GEL FILTRATION CHROMATOGRAPHY; GENE MUTATION; HYDROPHOBICITY; MOLECULAR STABILITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PH MEASUREMENT; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEQUENCE ANALYSIS; STREPTOMYCES; THERMOSTABILITY; THERMOTOGA; THERMUS THERMOPHILUS;

ACIDS; ALANINE; ALDOSE-KETOSE ISOMERASES; AMINO ACID SEQUENCE; ENZYME STABILITY; HEAT; HYDROGEN-ION CONCENTRATION; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; STREPTOMYCES;

BACTERIA (MICROORGANISMS); STREPTOMYCES; STREPTOMYCES OLIVOCHROMOGENES; STREPTOMYCES SP.; STREPTOMYCES SP. SK; THERMOTOGA; THERMOTOGA NEAPOLITANA; THERMUS; THERMUS THERMOPHILUS;

EID: 4544294824     PISSN: 03009084     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biochi.2004.07.003     Document Type: Article

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