Cold adaptation of xylose isomerase from Thermus thermophilus through random PCR mutagenesis: Gene cloning and protein characterization

European Journal of Biochemistry

Volumn 269, Issue 1, 2002, Pages 157-163

  Lönn, Anna ^a   Gárdonyi, Márk ^a   Van Zyl, Willem ^b   Hahn Hägerdal, Bärbel ^a   Otero, Ricardo Cordero ^b  

^a LUND UNIVERSITY   (Sweden)

^b STELLENBOSCH UNIVERSITY   (South Africa)

Author keywords

Cold adaptation; Random mutagenesis; Saccharomyces cerevisiae; Xylose fermentation; Xylose isomerase

Indexed keywords

GLUCOSE; MUTANT PROTEIN; XYLITOL; XYLOSE; XYLOSE ISOMERASE;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; COLD ACCLIMATIZATION; CONTROLLED STUDY; ENZYMATIC ASSAY; ENZYME ACTIVITY; ENZYME STABILITY; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION; GENETIC CODE; ISOMERISM; LOW TEMPERATURE; MOLECULAR CLONING; NONHUMAN; PH; PHYSICAL CHEMISTRY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; SITE DIRECTED MUTAGENESIS; THERMUS THERMOPHILUS;

ALDOSE-KETOSE ISOMERASES; COLD; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; KINETICS; MAGNESIUM; MANGANESE; MUTAGENESIS; POLYMERASE CHAIN REACTION; THERMUS THERMOPHILUS; XYLITOL;

ESCHERICHIA COLI; NEGIBACTERIA; PROKARYOTA; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE; THERMUS; THERMUS THERMOPHILUS; VECTORS;

EID: 0036150978     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.0014-2956.2002.02631.x     Document Type: Article

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