Multiplex RT-PCR amplification of HIV genes to create a completely autologous DC-based immunotherapy for the treatment of HIV infection

PLoS ONE

Volumn 3, Issue 1, 2008, Pages

  Tcherepanova, Irina ^a   Harris, Jason ^a   Starr, Aijing ^a   Cleveland, Jaclyn ^a   Ketteringham, Helen ^a   Calderhead, David ^a   Horvatinovich, Joe ^a   Healey, Don ^a   Nicolette, Charles A ^a  

^a Argos Therapeutics Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GAG PROTEIN; HUMAN IMMUNODEFICIENCY VIRUS ANTIGEN; NEF PROTEIN; REV PROTEIN; VIRUS RNA; VPR PROTEIN; PRIMER DNA;

ARTICLE; AUTOTRANSPLANTATION; CD8+ T LYMPHOCYTE; CELL TRANSPLANTATION; CONFOUNDING VARIABLE; CONTROLLED STUDY; DENDRITIC CELL; GENE AMPLIFICATION; GENE SEQUENCE; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; IMMUNE RESPONSE; IMMUNOTHERAPY; IN VITRO STUDY; INFECTION CONTROL; MULTIPLEX POLYMERASE CHAIN REACTION; MUTATION RATE; NONHUMAN; NUCLEOTIDE SEQUENCE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA TRANSCRIPTION; SEQUENCE ANALYSIS; T LYMPHOCYTE ACTIVATION; VIRUS GENE; VIRUS LOAD; VIRUS STRAIN; GENETICS; IMMUNOLOGY; METHODOLOGY;

BASE SEQUENCE; DENDRITIC CELLS; DNA PRIMERS; GENES, VIRAL; HIV; HIV INFECTIONS; HUMANS; IMMUNOTHERAPY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 45149125905     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0001489     Document Type: Article

References (36)

1. Ogg GS, Kostense S, Klein MR, Jurriaans S, Hamann D, et al (1999) Longitudinal Phenotypic Analysis of Human Immunodeficiency Virus Type 1-Specific Cytotoxic T Lymphocytes: Correlation with Disease Progression. J Virology 73: 9153-9160.
2. Jin X, Bauer DE, Tuttleton SE, Lewin S, Gettie A, et al. (1999) Dramatic rise in plasma viremia after CD8(+) T cell depletion in simian immunodeficiency virus-infected macaques. J Exp Med 189: 991-998.
3. Harter T, Harrer E, Kalams SA, Barbosa P, Trocha A, et al. (1996) Cytotoxic T' Lymphocytes in Asymptomatic Long-Term Nonprogressing HIV-1 Infection. The journal of Immunohgy 156: 2616-2623.
4. Evans DT, O'Connor DH, Jing P, Dzuris JL, Sidney J, et al. (1999) Virus-specific cytotoxic T-lymphocyte responses select for amino-acid variation in simian immunodeficiency virus Env and Nef. Nat Med 5: 1270-1276.
5. Zanussi S, Simonelli C, D'Andrea M, Caffau C, Clerici M, et al. (1996) CD8+ lymphocyte phenotype and cytokine production in long-term non-progressor and in progressor patients with HIV-1 infection. Clin Exp Immunol 105: 220-224.
6. Gamberg J, Pardoe I, Bowmer MI, Howley C, M. G (2004) Lack of CD28 expression on HIV-specific cytotoxic T lymphocytes is associated with disease progression. Immunol Cell Biol 82: 38-46.
7. Garber DA, Silvestri G, Feinberg MB (2004) Prospects for and AIDS vaccine: three big questions, no easy answers. The Lancet 4: 397-413.
8. McMichael AJ (2006) HIV vaccines. Annual Rev Immunol 24: 227-255.
9. Nabel GJ (2001) Challenges and opportunities for development of an AIDS vaccine. Nature 410: 1002-1007.
10. Altfeld M, Rosenberg ES, Shankarappa R, Mukherjee JS, Hecht FM, et al. (2001) Cellular Immune Responses and Viral Diversity in Individuals Treated during Acute and Early HIV-1 Infection. J Exp Med 193: 169-180.
11. Goulder PJR, Walker BD (1999) The great escape-AIDS viruses and immune control. Mature Medicine 5: 1233-1235.
12. Goulder PJR, I. Watkins D (2004) HIV AND SIV CTL ESCAPE: IMPLICATIONS FOR VACCINE DESIGN. Nature 4: 630-640.
13. Barouch DH, Jennifer K, Kuroda MJ, Schmitz JE, Santra S, et al. (2002) Eventual AIDS vaccine failure in rhesus monkey by viral escape from cytotoxic T lymphocytes. Nature 415: 335-339.
14. Garcia F, Lejeune M, Climent N, Gil C, Alcami J, et al. (2005) Therapeutic Immunization with Dendritic Cells Loaded with Heat-Inactivated Autologous HIV-1 in Patients with Chronic HIV-1 Infection. JID 191: 1680-1685.
15. Lu W, Arraes LC, Ferreira WT, Andrieu JM (2004) Therapeutic dendritic-cell vaccine for chronic HIV-1 infection. Nat Med 10: 1359-1365.
16. Addo NM, Yu XG, Rosenberg ES, Walker BD, Altfeld M (2002) Cytotoxic T-lymphocyte (CTL) responses directed against regulatory and accessory proteins in HIV-1 infection. DNA Cell Biol 21: 671-678.
17. Allen TM, Vogel TU, Fuller DH, Mothe BR, Steffen S, et al. (2000) Induction of AIDS virus-specific CTL activity in fresh, unstimulated peripheral blood lymphocytes from rhesus macaques vaccinated with a DNA prime/modified vaccinia virus Ankara boost regimen. J Immunol 164: 4968-4978.
18. Novitsky V, Cau H, Rybak N, Gilbert P, McLane MF, et al. (2002) Magnitude and frequency of cytotoxic T-lymphocyte responses: identification of immuno-dominant regions of human immunodeficiency virus type 1 subtype C. J Virol 76: 10155-10168.
19. Weissman D, Ni H, Scales D, Dude A, Capodici J, et al. (2000) HIV gag mRNA transfection of dendritic cells (DC) delivers encoded antigen to MHC class I and II molecules, causes DC maturation, and induces a potent human in vitro primary immune response. J Immunol 165: 4710-4717.
20. Altfeld M, Addo MM, Eldridge RL, Yu XG, Thomas S, et al (2001) Vpr Is Preferentially Targeted by CTL During HIV-1 Infection1. The Journal of Immunology 167: 2743-2752.
21. Hel Zk, Tsai W-P, Tryniszewska E, Nacsa J, Markham PD, et al. (2006) Improved Vaccine Protection from Simian AIDS by the Addition of Nonstructural Simian Immunodeficiency Virus Genes. The Journal of Immunology 176: 85-96.
22. Candotti D, Temple J, Owusu-Ofori S, Allain JP (2004) Multiplex real-time quantitative RT-PCR assay for hepatitis B virus, hepatitis C virus, and human immunodeficiency virus type 1. J Virol Methods 118: 39-47.
23. Palmer S, Wiegand AP, Maldarelli F, Bazmi H, Mican JM, et al. (2003) New real-time reverse transcriptase-initiated PCR assay with single-copy sensitivity for human immunodeficiency virus type 1 RNA in plasma. J Clin Microbiol 41: 4531-4536.
24. Erali M, Hillyard DR (t999) Evaluation of the ultrasensitive Roche Amplicor HIV-1 monitor assay for quantitation of human immunodeficiency virus type 1 RNA. J Clin Microbiol 37: 792-795.
25. Fang G, Weiser B, Kuiken C, Philpott SM, Rowland-Jones S, et al. (2004) Recombination following superinfection by HIV-1. Aids 18: 153-159.
26. Scriba TJ, zur Megede J. GRH, Treurnicht FK, Barnette SW, van Rensburg EG (2005) Functionally-inactive and immunogenic Tat, Rev and Nef DNA vaccines derived from sub-Saharan subtype C human immunodefficiency virus type I consensus sequences. Vaccine 23: 1158-1169.
27. Cohen WM, Bianco A, Connan F, Camoin L, Dadod M, et al. (2002) Study of antigen-processing steps reveals preferences explaining differential biological outcomes of two HLA-A2-restricted immunodominant epitopes from human immunodeficiency virus type 1. J Virol 76: 10219-10225.
28. Draenert R, Allen TM, Liu Y, Wrin T, Chappey C, et al. (2006) Constraints on HIV-1 evolution and immunodominance revealed in monozygotic adult twins infected with the same virus. J Exp Med 203: 529-539.
29. Su Z, Vieweg J, Weizer AZ, Dahm P, Yancey D, et al. (2002) Enhanced induction of telomerase-specific CD4(+) T cells using dendritic cells transfected with RNA encoding a chimeric gene product. Cancer Res 62: 5041-5048.
30. Singh RA, Barry MA (2004) Repertoire and immunofocusing of CD8 T cell responses generated by HIV-1 gag-pol and expression library immunization vaccines. J Immunol 173: 4387-4393.
31. Friedrich TC, Dodds EJ, Yant LJ, Vojnov L, Rudersdorf R, et al. (2004) Reversion of CTL escape-variant immunodeficiency viruses in vivo. Nat Med 10: 275-281.
32. Altschul SF, Madden TL, Schaffer AA, Zhang J, ZhangMiller ZW, et al. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25: 3389-3402.
33. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215: 403-410.
34. Christopherson C, Sninsky J, Kwok S (1997) The effects of internal primer-template mismatches on RT-PCR: HIV-1 model studies. Nucleic Acids Research 25: 654-658.
35. Chang P, Cantin E, Zaia J, Ladne P, Stephens D, et al. (1990) Ribozyme-mediated site-specific cleavage of the HIV-1 genome. Clin Biotechnol 2: 23-31.
36. Gao F, Robertson DL, Morrison SG, Hui H, Craig S, et al. (1996) The heterosexual human immunodeficiency virus type 1 epidemic in Thailand is caused by an intersubtype (A/E) recombinant of African Origin. J Virol 70: 7013-7029.