Yeast α-tubulin suppressor Ats1/Kti13 relates to the Elongator complex and interacts with Elongator partner protein Kti11

Molecular Microbiology

Volumn 69, Issue 1, 2008, Pages 175-187

  Zabel, René ^b   Bär, Christian ^a,b   Mehlgarten, Constance ^b   Schaffrath, Raffael ^a,b  

^a UNIVERSITY OF LEICESTER   (United Kingdom)

^b MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA TUBULIN; BACTERIAL TOXIN; ELONGATOR PROTEIN; KILLER TOXIN INSENSITIVE 13; RESTRICTION ENDONUCLEASE; TRANSCRIPTION ELONGATION FACTOR; TRANSFER RNA;

ALLELE; ALPHA TUBULIN SUPPRESOR 1; ARTICLE; CELL GROWTH; CELL VIABILITY; GENE DELETION; GENE EXPRESSION; GENETIC TRANSCRIPTION; GROWTH REGULATION; MISSENSE MUTATION; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN PROCESSING; RNA TRANSLATION; SACCHAROMYCES CEREVISIAE; SUPPRESSOR GENE; TRANSCRIPTION INITIATION; TRANSCRIPTION INITIATION SITE; TRANSCRIPTION TERMINATION; TRANSLATION REGULATION; YEAST;

BIOLOGICAL TRANSPORT; CELL CYCLE PROTEINS; CYTOPLASM; GENE EXPRESSION REGULATION, FUNGAL; KLUYVEROMYCES; MUTATION; MYCOTOXINS; NUCLEAR PROTEINS; PEPTIDE ELONGATION FACTORS; PROTEIN BINDING; REPRESSOR PROTEINS; RNA, TRANSFER; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SUPPRESSION, GENETIC;

KLUYVEROMYCES LACTIS; SACCHAROMYCES CEREVISIAE;

EID: 45149112613     PISSN: 0950382X     EISSN: 13652958     Source Type: Journal    
DOI: 10.1111/j.1365-2958.2008.06273.x     Document Type: Article

References (56)

1. Altmann, R. Kellogg, D. (1997) Control of mitotic events by Nap1 and the Gin4 kinase. J Cell Biol 138 : 119 130.
2. Andrews, P.D. Stark, M.J. (2000) Type 1 protein phosphatase is required for maintenance of cell wall integrity, morphogenesis and cell cycle progression in Saccharomyces cerevisiae. J Cell Sci 113 : 507 520.
3. Barlowe, C. Schekman, R. (1993) SEC12 encodes a guanine-nucleotide- exchange factor essential for transport vesicle budding from the ER. Nature 365 : 347 349.
4. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72 : 248 254.
5. Butler, A.R., White, J.H., Folawiyo, Y., Edlin, A., Gardiner, D. Stark, M.J. (1994) Two Saccharomyces cerevisiae genes which control sensitivity to G1 arrest induced by Kluyveromyces lactis toxin. Mol Cell Biol 14 : 6306 6316.
6. Chuong, S.D., Good, A.G., Taylor, G.J., Freeman, M.C., Moorhead, G.B. Muench, D.G. (2004) Large-scale identification of tubulin-binding proteins provides insight on subcellular trafficking, metabolic channeling and signaling in plant cells. Mol Cell Proteomics 3 : 970 983.
7. d'Enfert, C., Wuestehube, L.J., Lila, T. Schekman, R. (1991) Sec12p-dependent membrane binding of the small GTP-binding protein Sar1p promotes formation of transport vesicles from the ER. J Cell Biol 114 : 663 670.
8. Esberg, A., Huang, B., Johansson, M.J. Bystrom, A.S. (2006) Elevated levels of two tRNA species bypass the requirement for Elongator complex in transcription and exocytosis. Mol Cell 24 : 139 148.
9. Fichtner, L. Schaffrath, R. (2002) KTI11 and KTI13, Saccharomyces cerevisiae genes controlling sensitivity to G1 arrest induced by Kluyveromyces lactis zymocin. Mol Microbiol 44 : 865 875.
10. Fichtner, L., Frohloff, F., Bürkner, K., Larsen, M., Breunig, K.D. Schaffrath, R. (2002) Molecular analysis of KTI12/TOT4, a Saccharomyces cerevisiae gene required for Kluyveromyces lactis zymocin action. Mol Microbiol 43 : 783 791.
11. Fichtner, L., Jablonowski, D., Schierhorn, A., Kitamoto, H.K., Stark, M.J. Schaffrath, R. (2003) Elongator's toxin-target (TOT) function is nuclear localization sequence dependent and suppressed by post-translational modification. Mol Microbiol 49 : 1297 1307.
12. Frohloff, F., Fichtner, L., Jablonowski, D., Breunig, K.D. Schaffrath, R. (2001) Saccharomyces cerevisiae Elongator mutations confer resistance to the Kluyveromyces lactis zymocin. EMBO J 20 : 1993 2003.
13. Gardiner, J., Barton, D., Marc, J. Overall, R. (2007) Potential role of tubulin acetylation and microtubule-based protein trafficking in famialial dysautonomia. Traffic 8 : 1145 1149.
14. Gietz, D., St. Jean, A., Woods, R.A. Schiestl, R.H. (1992) Improved method for high efficiency transformation of intact yeast cells. Nucl Acids Res 20 : 1425.
15. Gietz, R.D. Sugino, A. (1988) New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74 : 527 534.
16. Gilbert, C., Kristjuhan, A., Winkler, G.S. Svejstrup, J.Q. (2004) Elongator interactions with nascent mRNA revealed by RNA immunoprecipitation. Mol Cell 14 : 457 464.
17. Grossenbacher, A.M., Stadelmann, B., Heyer, W.D., Thuriaux, P., Kohli, J., Smith, C. et al. (1986) Antisuppressor mutations and sulfur-carrying nucleosides in transfer RNAs of Schizosaccharomyces pombe. J Biol Chem 261 : 16351 16355.
18. Heyer, W.D., Thuriaux, P., Kohli, J., Ebert, P., Kersten, H., Gehrke, C. et al. (1984) An antisuppressor mutation of Schizosaccharomyces pombe affects the post-transcriptional modification of the 'wobble' base in the anticodon of tRNAs. J Biol Chem 259 : 2856 2862.
19. Hopper, A.K. Phizicky, E.M. (2003) tRNA transfers to the limelight. Genes Dev 17 : 162 180.
20. Huang, B., Johanson, M.J. Bystrom, A.S. (2005) An early step in wobble uridine tRNA modification requires the Elongator complex. RNA 11 : 424 436.
21. Jablonowski, D. Schaffrath, R. (2007) Zymocin, a composite chitinase and tRNAse killer toxin from yeast. Biochem Soc Trans 35 : 1533 1537.
22. Jablonowski, D., Frohloff, F., Fichtner, L., Stark, M.J. Schaffrath, R. (2001) Kluyveromyces lactis zymocin mode of action is linked to RNA polymerase II function via Elongator. Mol Microbiol 42 : 1095 1105.
23. Jablonowski, D., Fichtner, L., Frohloff, F. Schaffrath, R. (2003) Chitin binding capability of the zymocin complex from Kluyveromyces Lactis. In : Non-Conventional Yeasts in Genetics, Biochemistry and Biotechnology. Wolf, K, Breunig, K.D. Barth, G. (eds). Heidelberg, Berlin : Springer-Verlag, pp. 191 194.
24. Jablonowski, D., Fichtner, L., Stark, M.J. Schaffrath, R. (2004) The yeast Elongator histone acetylase requires Sit4-dependent dephosphorylation for toxin-target capacity. Mol Biol Cell 15 : 1459 1469.
25. Glu wobble uridine methylation by Trm9 identifies Elongator's key role for zymocin-induced cell death in yeast. Mol Microbiol 59 : 677 688.
26. Kellogg, D.R. Murray, A.W. (1995) NAP1 acts with Clb2 to perform mitotic functions and to suppress polar bud growth in budding yeast. J Cell Biol 130 : 675 685.
27. Kirkpatrick, D. Solomon, F. (1994) Overexpression of yeast homologs of the mammalian checkpoint gene RCC1 suppresses the class α-tubulin mutations that arrest with excess microtubules. Genetics 137 : 381 392.
28. Kishida, M., Tokunaga, M., Katayose, Y., Yajima, H., Kawamura-Watabe, A. Hishinuma, F. (1996) Isolation and genetic characterization of pGKL killer-insensitive mutants (iki) from Saccharomyces cerevisiae. Biosci Biotechnol Biochem 60 : 798 801.
29. Kong, S.E., Kobor, M.S., Krogan, N.J., Somesh, B.P., Sogaard, T.M., Greenblatt, J.F. Svejstrup, J.Q. (2005) Interaction of Fcp1 phosphatase with elongating RNA polymerase II holoenzyme, enzymatic mechanism of action, and genetic interaction with elongator. J Biol Chem 280 : 4299 4306.
30. Krogan, N.J., Cagney, G., Yu, H., Zhong, G., Guo, X., Ignatchenko, A. et al. (2006) Global landscape of protein complexes in the yeast Saccharomyces cerevisiae. Nature 440 : 637 643.
31. Liu, S., Milne, G.T., Kuremsky, J.G., Fink, G.R. Leppla, S.H. (2004) Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2. Mol Cell Biol 24 : 9487 9497.
32. Longtine, M.S., McKenzie, A., 3rd, Demarini, D.J., Shah, N.G., Wach, A., Brachat, A. et al. (1998) Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast 14 : 953 961.
33. Lu, J., Huang, B., Esberg, A., Johansson, M.J. Bystrom, A.S. (2005) The Kluyveromyces lactis gamma-toxin targets tRNA anticodons. RNA 11 : 1648 1654.
34. Mehlgarten, C. Schaffrath, R. (2003) Mutant casein kinase I (Hrr25p/Kti14p) abrogates the G1 cell cycle arrest induced by Kluyveromyces lactis zymocin in budding yeast. Mol Genet Genomics 269 : 188 196.
35. Murakami, A., Kimura, K. Nakano, A. (1999) The inactive form of a yeast casein kinase I suppresses the secretory defect of the sec12 mutant. J Biol Chem 274 : 3804 3810.
36. Nair, J., Müller, H., Peterson, M. Novick, P. (1990) Sec2 protein contains a coiled-coil domain essential for vesicular transport and a dispensable carboxy terminal domain. J Cell Biol 110 : 1897 1909.
37. Nelissen, H., Fleury, D., Bruno, L., Robles, P., De Veylder, L., Traas, J. et al. (2005) The elongata mutants identify a functional Elongator complex in plants with a role in cell proliferation during organ growth. Proc Natl Acad Sci USA 102 : 7754 7759.
38. Otero, G., Fellows, J., Li, Y., de Bizemont, T., Dirac, A.M., Gustafsson, C.M. et al. (1999) Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation. Mol Cell 3 : 109 118.
39. Pereira, G., Knop, M. Schiebel, E. (1998) Spc98p directs the yeast γ-tubulin complex into the nucleus and is subject to cell cycle-dependent phosphorylation on the nuclear side of the spindle pole body. Mol Biol Cell 9 : 775 793.
40. Petrakis, T.G., Sogaard, T.M., Erdjument-Bromage, H., Tempst, P. Svejstrup, J.Q. (2005) Physical and functional interaction between Elongator and the chromatin-associated Kti12 protein. J Biol Chem 280 : 19454 19460.
41. Pokholok, D.K., Hannett, N.M. Young, R.A. (2002) Exchange of RNA polymerase II initiation and elongation factors during gene expression in vivo. Mol Cell 9 : 799 809.
42. Ptacek, J., Devgan, G., Michaud, G., Zhu, H., Zhu, X., Fasolo, J. et al. (2005) Global analysis of protein phosphorylation in yeast. Nature 438 : 679 684.
43. Rafalski, A., Kohli, J., Agris, P. Soll, D. (1979) The nucleotide sequence of a UGA suppressor serine tRNA from Schizosaccharomyces pombe. Nucl Acids Res 6 : 2683 2695.
44. Rahl, P.B., Chen, C.Z. Collins, R.N. (2005) Elp1p, the yeast homolog of the FD disease syndrome protein, negatively regulates exocytosis independently of transcriptional elongation. Mol Cell 17 : 841 853.
45. Sherman, F. (1991) Getting started with yeast. Meth Enzymol 194 : 3 21.
46. Shields, C.M., Taylor, R., Nazarenus, T., Cheatle, J., Hou, A., Tapprich, A. et al. (2003) Saccharomyces cerevisiae Ats1p interacts with Nap1p, a cytoplasmic protein that controls bud morphogenesis. Curr Genet 44 : 184 194.
47. Sjölinder, M., Uhlmann, J. Ponstingl, H. (2002) DelGEF, a homologue of the Ran guanine nucleotide exchange factor RanGEF, binds to the exocyst component Sec5 and modulates secretion. FEBS Lett 532 : 211 215.
48. Sjölinder, M., Uhlmann, J. Ponstingl, H. (2004) Characterisation of an evolutionary conserved protein interacting with the putative guanine nucleotide exchange factor DelGEF and modulating secretion. Exp Cell Res 294 : 68 76.
49. Su, J.Y., Belmont, L. Sclafani, R.A. (1990) Genetic and molecular analysis of the SOE1 gene: a tRNA (3Glu) missense suppressor of yeast cdc8 mutations. Genetics 124 : 523 531.
50. Svejstrup, J.Q. (2007) Elongator complex: how many roles does it play? Curr Opin Cell Biol 19 : 331 336.
51. Thuriaux, P., Minet, M., Hofer, F. Leupold, U. (1976) Genetic analysis of antisuppressor mutants in the fission yeast Schizosaccharomyces pombe. Mol Gen Genet 142 : 251 261.
52. Tong, A.H., Evangelista, M., Parsons, A.B., Xu, H., Bader, G.D., Page, N. et al. (2001) Systematic genetic analysis with ordered arrays of yeast deletion mutants. Science 294 : 2364 2368.
53. Tong, A.H., Lesage, G., Bader, G.D., Ding, H., Xu, H., Xin, X. et al. (2004) Global mapping of the yeast genetic interaction network. Science 303 : 808 813.
54. Wetzel, R., Kohli, J., Altruda, F. Soll, D. (1979) Identification and nucleotide sequence of the sup8-e UGA-suppressor leucine tRNA from Schizosaccharomyces pombe. Mol Gen Genet 172 : 221 228.
55. Winkler, G.S., Petrakis, T.G., Ethelberg, S., Tokunaga, M., Erdjument-Bromage, H., Tempst, P. Svejstrup, J.Q. (2001) RNA polymerase II elongator holoenzyme is composed of two discrete subcomplexes. J Biol Chem 276 : 32743 32749.
56. Zachariae, W., Shin, T.H., Galova, M., Obermaier, B. Nasmyth, K. (1996) Identification of subunits of the anaphase-promoting complex of Saccharomyces cerevisiae. Science 274 : 1201 1204.