Glutamate-induced glioma cell proliferation is prevented by functional expression of the glutamate transporter GLT-1

FEBS Letters

Volumn 582, Issue 13, 2008, Pages 1847-1852

  Vanhoutte, Nicolas ^a   Hermans, Emmanuel ^a  

^a UNIVERSITÉ CATHOLIQUE DE LOUVAIN   (Belgium)

Author keywords

Astrocyte; Excitotoxicity; Glial tumour; Glutamate uptake; Inducible expression

Indexed keywords

ASPARTIC ACID; CYSTINE; DOXYCYCLINE; GLUTAMIC ACID; VESICULAR GLUTAMATE TRANSPORTER 1;

ANIMAL CELL; ARTICLE; ASTROCYTE; CELL PROLIFERATION; CONTROLLED STUDY; GLIOMA CELL; NEWBORN; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT;

ANIMALS; CELL LINE, TUMOR; CELL PROLIFERATION; EXCITATORY AMINO ACID TRANSPORTER 2; GLIOMA; GLUTAMIC ACID; RATS; TRANSFECTION;

EID: 44449153822     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2008.04.053     Document Type: Article

References (25)

1. Kanner B.I. Glutamate transporters from brain. A novel neurotransmitter transporter family. FEBS Lett. 325 (1993) 95-99
2. Rothstein J.D., Dykes-Hoberg M., Pardo C.A., Bristol L.A., Jin L., Kuncl R.W., Kanai Y., Hediger M.A., Wang Y., Schielke J.P., and Welty D.F. Knockout of glutamate transporters reveals a major role for astroglial transport in excitotoxicity and clearance of glutamate. Neuron 16 (1996) 675-686
3. Honig L.S., Chambliss D.D., Bigio E.H., Carroll S.L., and Elliott J.L. Glutamate transporter EAAT2 splice variants occur not only in ALS, but also in AD and controls. Neurology 55 (2000) 1082-1088
4. Munch C., Penndorf A., Schwalenstocker B., Troost D., Ludolph A.C., Ince P., and Meyer T. Impaired RNA splicing of 5'-regulatory sequences of the astroglial glutamate transporter EAAT2 in human astrocytoma. J. Neurol. Neurosurg. Psychiatr. 71 (2001) 675-678
5. Ye Z.C., Rothstein J.D., and Sontheimer H. Compromised glutamate transport in human glioma cells: reduction-mislocalization of sodium-dependent glutamate transporters and enhanced activity of cystine-glutamate exchange. J. Neurosci. 19 (1999) 10767-10777
6. de Groot J.F., Liu T.J., Fuller G., and Yung W.K. The excitatory amino acid transporter-2 induces apoptosis and decreases glioma growth in vitro and in vivo. Cancer Res. 65 (2005) 1934-1940
7. Behrens P.F., Langemann H., Strohschein R., Draeger J., and Hennig J. Extracellular glutamate and other metabolites in and around RG2 rat glioma: an intracerebral microdialysis study. J. Neurooncol. 47 (2000) 11-22
8. Sato H., Tamba M., Ishii T., and Bannai S. Cloning and expression of a plasma membrane cystine/glutamate exchange transporter composed of two distinct proteins. J. Biol. Chem. 274 (1999) 11455-11458
9. Ye Z.C., and Sontheimer H. Glioma cells release excitotoxic concentrations of glutamate. Cancer Res. 59 (1999) 4383-4391
10. Arcella A., Carpinelli G., Battaglia G., D'Onofrio M., Santoro F., Ngomba R.T., Bruno V., Casolini P., Giangaspero F., and Nicoletti F. Pharmacological blockade of group II metabotropic glutamate receptors reduces the growth of glioma cells in vivo. Neuro-Oncology 7 (2005) 236-245
11. D'Onofrio M., Arcella A., Bruno V., Ngomba R.T., Battaglia G., Lombari V., Ragona G., Calogero A., and Nicoletti F. Pharmacological blockade of mGlu2/3 metabotropic glutamate receptors reduces cell proliferation in cultured human glioma cells. J. Neurochem. 84 (2003) 1288-1295
12. Ishiuchi S., Tsuzuki K., Yoshida Y., Yamada N., Hagimura N., Okado H., Miwa A., Kurihara H., Nakazato Y., Tamura M., Sasaki T., and Ozawa S. Blockage of Ca(2+)-permeable AMPA receptors suppresses migration and induces apoptosis in human glioblastoma cells. Nat. Med. 8 (2002) 971-978
13. Rzeski W., Turski L., and Ikonomidou C. Glutamate antagonists limit tumor growth. Proc. Natl. Acad. Sci. USA 98 (2001) 6372-6377
14. Tilleux S., Berger J., and Hermans E. Induction of astrogliosis by activated microglia is associated with a down-regulation of metabotropic glutamate receptor 5. J. Neuroimmunol. 189 (2007) 23-30
15. Vermeiren C., Hemptinne I., Vanhoutte N., Tilleux S., Maloteaux J.M., and Hermans E. Loss of metabotropic glutamate receptor-mediated regulation of glutamate transport in chemically activated astrocytes in a rat model of amyotrophic lateral sclerosis. J. Neurochem. 96 (2006) 719-731
16. Vanhoutte N., de H.I., Vermeiren C., Maloteaux J.M., and Hermans E. In vitro differentiated neural stem cells express functional glial glutamate transporters. Neurosci. Lett. 370 (2004) 230-235
17. Najimi M., Maloteaux J.M., and Hermans E. Cytoskeleton-related trafficking of the EAAC1 glutamate transporter after activation of the G(q/11)-coupled neurotensin receptor NTS1. FEBS Lett. 523 (2002) 224-228
18. Abe K., and Matsuki N. Measurement of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction activity and lactate dehydrogenase release using MTT. Neurosci. Res. 38 (2000) 325-329
19. Sims K.D., Straff D.J., and Robinson M.B. Platelet-derived growth factor rapidly increases activity and cell surface expression of the EAAC1 subtype of glutamate transporter through activation of phosphatidylinositol 3-kinase. J. Biol. Chem. 275 (2000) 5228-5237
20. Hussain R.F., Nouri A.M., and Oliver R.T. A new approach for measurement of cytotoxicity using colorimetric assay. J. Immunol. Methods 160 (1993) 89-96
21. Palos T.P., Ramachandran B., Boado R., and Howard B.D. Rat C6 and human astrocytic tumor cells express a neuronal type of glutamate transporter. Brain Res. Mol. Brain Res. 37 (1996) 297-303
22. Rothstein J.D., Martin L., Levey A.I., Dykes-Hoberg M., Jin L., Wu D., Nash N., and Kuncl R.W. Localization of neuronal and glial glutamate transporters. Neuron 13 (1994) 713-725
23. Kato S., Negishi K., Mawatari K., and Kuo C.H. A mechanism for glutamate toxicity in the C6 glioma cells involving inhibition of cystine uptake leading to glutathione depletion. Neuroscience 48 (1992) 903-914
24. Yao H.H., Ding J.H., Zhou F., Wang F., Hu L.F., Sun T., and Hu G. Enhancement of glutamate uptake mediates the neuroprotection exerted by activating group II or III metabotropic glutamate receptors on astrocytes. J. Neurochem. 92 (2005) 948-961
25. Palos T.P., Zheng S., and Howard B.D. Wnt signaling induces GLT-1 expression in rat C6 glioma cells. J. Neurochem. 73 (1999) 1012-1023