A homogeneous time-resolved fluorescence detection of telomerase activity

Analytical Biochemistry

Volumn 333, Issue 1, 2004, Pages 105-113

  Gabourdes, Manuel ^a   Bourgine, Valérie ^a   Mathis, Gérard ^a   Bazin, Hervé ^a   Alpha Bazin, Béatrice ^b  

^a CIS Bio International   (France)

^b CEA Marcoule   (France)

Author keywords

Europium cryptate; FRET; LRET; Telomerase; Time resolved fluorescence; TRAP

Indexed keywords

ENERGY TRANSFER; EUROPIUM COMPOUNDS; OLIGONUCLEOTIDES; PROTEINS;

FRET; LRET; TELOMERASE; TIME-RESOLVED FLUORESCENCE; TRAP;

FORSTER RESONANCE ENERGY TRANSFER;

ALLOPHYCOCYANINE; ANTHRAQUINONE DERIVATIVE; DEOXYURIDINE TRIPHOSPHATE DERIVATIVE; EUROPIUM; OLIGONUCLEOTIDE; PHYCOCYANIN; STREPTAVIDIN; TELOMERASE; TELOMERASE INHIBITOR; UNCLASSIFIED DRUG;

ARTICLE; BIOTINYLATION; CHEMICAL LABELING; CONJUGATION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME SUBSTRATE; FLUORESCENCE; FLUORESCENCE RESONANCE ENERGY TRANSFER; HOMOGENEOUS TIME RESOLVED FLUORESCENCE; HUMAN; HUMAN CELL; IC 50; MEASUREMENT; MONITORING; PRIORITY JOURNAL; REACTION ANALYSIS; SCREENING; TELOMERIC REPEAT AMPLIFICATION PROTOCOL; TIME; VALIDATION PROCESS;

EID: 4444370945     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2004.06.006     Document Type: Article

References (35)

1. C.W. Greider, and E.H. Blackburn Identification of a specific telomere terminal transferase activity in Tetrahymena extracts Cell 43 1985 405 413
2. G.B. Morin The human telomere terminal transferase enzyme is a ribonucleoprotein that synthesizes TTAGGG repeats Cell 59 1989 521 529
3. M. Meyerson, C.M. Counter, E.N. Eaton, L.W. Ellisen, P. Steiner, S.D. Caddle, L. Ziaugra, R.L. Beijersbergen, M.J. Davidoff, Q. Liu, S. Bacchetti, D.A. Haber, and R.A. Weinberg hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization Cell 90 1997 785 795
4. A. Kilian, D.D. Bowtell, H.E. Abud, G.R. Hime, D.J. Venter, P.K. Keese, E.L. Duncan, R.R. Reddel, and R.A. Jefferson Isolation of a candidate human telomerase catalytic subunit gene, which reveals complex splicing patterns in different cell types Hum. Mol. Genet. 6 1997 2011 2019
5. T.M. Nakamura, G.B. Morin, K.B. Chapman, S.L. Weinrich, W.H. Andrews, J. Lingner, C.B. Harley, and T.R. Cech Telomerase catalytic subunit homologs from fission yeast and human Anal. Biochem. 277 1997 955 959
6. J. Feng, W.D. Funk, S.S. Wang, S.L. Weinrich, A.A. Avilion, C.P. Chiu, R.R. Adams, E. Chang, R.C. Allsopp, and J. Yu The RNA component of human telomerase Science 269 1995 1236 1241
7. R.J. Wellinger, and D. Sen The DNA structures at the ends of eukaryotic chromosomes Eur. J. Cancer 33 1997 735 749
8. S. Huard, and C. Autexier Targeting human telomerase in cancer therapy Curr. Med. Chem. Anti-Canc. Agents 2 2002 577 587
9. N.W. Kim, M.A. Piatyszek, K.R. Prowse, C.B. Harley, M.D. West, P.L. Ho, G.M. Coviello, W.E. Wright, S.L. Weinrich, and J.W. Shay Specific association of human telomerase activity with immortal cells and cancer Science 266 1994 2011 2015
10. S. Xu, M. He, H. Yu, X. Cai, X. Tan, B. Lu, and B. Shu A quantitative method to measure telomerase activity by bioluminescence connected with telomeric repeat amplification protocol Anal. Biochem. 299 2001 188 193
11. H. Wege, M.S. Chui, H.T. Le, J.M. Tran, and M.A. Zern SYBR Green real-time telomeric repeat amplification protocol for the rapid quantification of telomerase activity Nucleic Acids Res. 31 2003 E3-3
12. J.L. Mergny, J.F. Riou, P. Mailliet, M.P. Teulade-Fichou, and E. Gilson Natural and pharmacological regulation of telomerase Nucleic Acids Res. 30 2002 839 865
13. D. Sun, L.H. Hurley, and D.D. Von Hoff Telomerase assay using biotinylated-primer extension and magnetic separation of the products Biotechniques 25 1998 1046 1051
14. B. Alpha, J.M. Lehn, and G. Mathis Energy transfer luminescence of europium(III) and terbium(III) cryptates of macrobicyclic polypyridine ligands Angew. Chem. Int. Ed. Engl. 26 1987 266 267
15. G. Mathis Probing molecular interactions with homogeneous techniques based on rare earth cryptates and fluorescence energy transfer Clin. Chem. 41 1995 1391 1397
16. B. Alpha-Bazin, and G. Mathis New homogeneous reverse transcriptase and nuclease assays based on rare earth cryptate and fluorescent energy transfer Nucleosides Nucleotides 18 1999 1277 1278
17. B. Alpha-Bazin, H. Bazin, S. Guillemer, S. Sauvaigo, and G. Mathis Europium cryptate labeled deoxyuridine-triphosphate analog: synthesis and enzymatic incorporation Nucleosides Nucleotides Nucleic Acids 19 2000 1463 1474
18. H. Bazin, E. Trinquet, and G. Mathis Time resolved amplification of cryptate emission: a versatile technology to trace biomolecular interactions J. Biotechnol. 82 2002 233 250
19. N.W. Kim Clinical implications of telomerase in cancer Eur. J. Cancer 33 1997 781 786
20. A. Roget, H. Bazin, and R. Teoule Synthesis and use of labelled nucleoside phosphoramidite building blocks bearing a reporter group: biotinyl, dinitrophenyl, pyrenyl and dansyl Nucleic Acids Res. 17 1989 7643 7651
21. H. Uehara, G. Nardone, I. Nazarenko, and R.J. Hohman Detection of telomerase activity utilizing energy transfer primers: comparison with gel- and ELISA-based detection Biotechniques 26 1999 552 558
22. G. Mathis Rare earth cryptates and homogeneous fluoroimmunoassays with human sera Clin. Chem. 39 1993 1953 1959
23. T. Förster Zwischen molekulare energiewanderung und fluoreszenz Ann. Phys. 2 1948 55 75
24. L. Stryer Fluorescence energy transfer as a spectroscopic ruler Annu. Rev. Biochem. 47 1978 819 846
25. P.R. Selvin Fluorescence resonance energy transfer Methods in Enzymology 1995 Academic Press San Diego 300 334
26. L. Stryer, D.D. Thomas, and C.F. Meares Fluorescence energy transfer in the rapid-diffusion limit Proc. Natl. Acad. Sci. USA 75 1978 5746 5750
27. L. Stryer, D.D. Thomas, and C.F. Meares Diffusion-enhanced fluorescence energy transfer Annu. Rev. Biophys. Bioeng. 11 1982 203 222
28. M.L. Jones, and G.P. Kurzban Noncooperativity of biotin binding to tetrameric streptavidin Biochemistry 34 1995 11750 11756
29. M. Gonzalez, L.A. Bagatolli, I. Echabe, J.L. Arrondo, C.E. Argarana, C.R. Cantor, and G.D. Fidelio Interaction of biotin with streptavidin. Thermostability and conformational changes upon binding J. Biol. Chem. 272 1997 11288 11294
30. J.M. Wen, L.B. Sun, M. Zhang, and M.H. Zheng A non-isotopic method for the detection of telomerase activity in tumour tissues: TRAP-silver staining assay Mol. Pathol. 51 1998 110 112
31. D.H. Gelfand Taq DNA polymerase H.A. Erlich PCR Technology: Principles and Applications for DNA Amplification 1989 Stockton Press New York 17 22
32. D. Sun, B. Thompson, B.E. Cathers, M. Salazar, S.M. Kerwin, J.O. Trent, T.C. Jenkins, S. Neidle, and L.H. Hurley Inhibition of human telomerase by a G-quadruplex-interactive compound J. Med. Chem. 40 1997 2113 2116
33. P.J. Perry, S.M. Gowan, A.P. Reszka, P. Polucci, T.C. Jenkins, L.R. Kelland, and S. Neidle 1,4- and 2,6-disubstituted amidoanthracene-9,10-dione derivatives as inhibitors of human telomerase J. Med. Chem. 41 1998 3253 3260
34. J.E. Mata, S.S. Joshi, B. Palen, S.J. Pirruccello, J.D. Jackson, N. Elias, T.J. Page, K.L. Medlin, and P.L. Iversen A hexameric phosphorothioate oligonucleotide telomerase inhibitor arrests growth of Burkitt's lymphoma cells in vitro and in vivo Toxicol. Appl. Pharmacol. 144 1997 189 197
35. B. Alpha-Bazin, H. Bazin, M. Préaudat, E. Trinquet, and G. Mathis Rare earth cryptates and trace technology as tools for probing molecular interactions in biology B. Valeur J.-C. Brochon New Trends in Fluorescence Spectroscopy: Applications to Chemical and Life Sciences 2001 Springer Berlin 439 480