Telomerase assay using biotinylated-primer extension and magnetic separation of the products

BioTechniques

Volumn 25, Issue 6, 1998, Pages 1046-1051

  Sun, Daekyu ^a   Hurley, Laurence H ^a   Von Hoff, Daniel D ^b  

^a CTRC Institute for Drug Development   (United States)

^b UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RIBONUCLEOPROTEIN; TELOMERASE;

ARTICLE; BIOTINYLATION; CANCER CELL; CANCER CELL DESTRUCTION; CONTROLLED STUDY; DRUG TARGETING; ENZYME ASSAY; HELA CELL; HUMAN; HUMAN CELL;

EID: 0032414170     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98256cr03     Document Type: Article

References (17)

1. Blackburn, E.H. 1991. Structure and function of telomeres. Nature 350:569-573.
2. Blackburn, E.H. 1992. Telomerases. Annu. Rev. Biochem. 61:113-129.
3. Blackburn, E.H. and C.W. Greidcr (Eds). 1995. Telomeres, CHS Laboratory Press, Cold Spring Harbor, NY.
4. Counter, C.M., A.A. Avilion, C.E. LeFeuvre, N.G. Stewart, C.W. Greider, C.B. Harley and S. Bacchetti. 1992. Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity. EMBO J. 11:1921-1929.
5. Counter, C.M., H.W. Hirte, S. Bacchetti and C.B. Harley. 1994. Telomerase activity in human ovarian carcinoma. Proc. Natl. Acad. Sci. USA 91:2900-2904.
6. de Lange, T. 1994. Activation of telomerase in human tumor. Proc. Natl. Acad. Sci. USA 91:2882-2885.
7. Greider, C.W. and E.H. Blackburn. 1985. Identification of a specific telomere terminal transferase activity in Tetrahymena extracts. Cell 43:405-413.
8. Greider, C.W. and E.H. Blackburn. 1987. A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis. Nature 337:331-337.
9. Greider, C.W. and E.H. Blackburn. 1987. The telomere terminal transferase of Tetrahymena is a ribonucleoprotein enzyme with two kinds of primer specificity. Cell 51:887-898.
10. Harley, C.B., A.B. Futcher and C.W. Greider. 1990. Telomeres shorten during aging of human fibroblasts. Nature 345:458-460.
11. Hiyama, E., T. Yokojama, N. Tatsumoto, K. Hiyama, Y. Imamura, Y. Murakami, T. Kodama, M.A. Piatyszek, J.W. Shay and Y. Matsuura. 1995. Telomerase activity in gastric cancer. Cancer Res. 55:3258-3262.
12. Kim, N.W., M.A. Piatyszek, K.R. Prowse, C.B. Harley, M.D. West, P.L.C. Ho, G.M. Coviello, W.E. Wright, S.L. Weinrich and J.W. Shay. 1994. Specific association of human telomerase activity with immortal cells and cancer. Science 266:2011-2015.
13. Levy, M.Z., R.C. Allsopp, B. Futcher, C.W. Greider and C.B. Harley. 1992. Telomere end-replication problem and cell aging. J. Mol. Biol. 225:951-960.
14. Morin, G.B. 1989. The human telomere terminal transferase enzyme is a ribonucleoprotein that synthesizes TTAGGG repeats. Cell 59:521-529.
15. Prowse, K.R., A.A. Avilion and C.W. Greider. 1993. Identification of a nonprocessive telomerase activity from mouse cells. Proc. Natl. Acad. Sci. USA 90:1493-1497.
16. Shay, J.W., H. Werbin and W.E. Wright. 1994. Telomere shortening may contribute to aging and cancer: a perspective. Mol. Cell. Diff. 2:1-21.
17. Watson, J.D. 1972. Origin of concatemeric T7 DNA. Nature New Biol. 239:197-201.