Expression of LEKTI domains 6-9′ in the baculovirus expression system: Recombinant LEKTI domains 6-9′ inhibit trypsin and subtilisin A

Protein Expression and Purification

Volumn 35, Issue 1, 2004, Pages 93-101

  Jayakumar, Arumugam ^a   Kang, Ya'an ^a   Mitsudo, Kenji ^b   Henderson, Ying ^a   Frederick, Mitchell J ^a   Wang, Mary ^a   El Naggar, Adel K ^a   Marx, Ute C ^c   Briggs, Katrina ^a   Clayman, Gary L ^a  

^a UNIVERSITY OF TEXAS   (United States)

^b NAGOYA UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^c UNIVERSITY OF BAYREUTH   (Germany)

Author keywords

Baculovirus; LEKTI; LEKTI domains; Noncompetitive inhibition; Serine proteinases; SPINK5

Indexed keywords

UNIDENTIFIED BACULOVIRUS; VECTORS;

EID: 4444223718     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2003.12.004     Document Type: Article

References (30)

1. H.J. Magert, L. Standker, P. Kreutzmann, H.D. Zucht, M. Reinecke, C.P. Sommerhoff, H. Fritz, W.G. Forssmann, LEKTI, a novel 15-domain type of human serine proteinase inhibitor, J. Biol. Chem. 274 (1999) 21499-21502.
2. H.J. Magert, P. Kreutzmann, L. Standker, M. Walden, K. Drogemuller, W.G. Forssmann, LEKTI: a multidomain serine proteinase inhibitor with pathophysiological relevance, Int. J. Biochem. Cell. Biol. 34 (2002) 573-576.
3. H.J. Magert, P. Kreutzmann, K. Drogemuller, L. Standker, K. Adermann, M. Walden, H. John, H.C. Korting, W.G. Forssmann, The 15-domain serine proteinase inhibitor LEKTI: biochemical properties, genomic organization, and pathophysiological role, Eur. J. Med. Res. 7 (2002) 49-56.
4. A. Ahmed, P. Kandola, G. Ziada, N. Parenteau, Purification and partial amino acid sequence of proteins from human epidermal keratinocyte conditioned medium, J. Protein Chem. 20 (2001) 273-278.
5. T. Lauber, A. Schulz, K. Schweimer, K. Adermann, U.C. Marx, Homologous proteins with different folds: the three-dimensional structures of domains 1 and 6 of the multiple Kazal-type inhibitor LEKTI, J. Mol. Biol. 328 (2003) 205-219.
6. S. Chavanas, C. Bodemer, A. Rochat, D. Hamel-Teillac, M. Ali, A.D. Irvine, J.L. Bonafe, J. Wilkinson, A. Taieb, Y. Barrandon, J.I. Harper, Y. de Prost, A. Hovnanian, Mutations in SPINK5, encoding a serine protease inhibitor, cause Netherton syndrome, Nat. Genet. 25 (2000) 141-142.
7. F.B. Muller, I. Hausser, D. Berg, C. Casper, R. Maiwald, A. Jung, H. Jung, B.P. Korge, Genetic analysis of a severe case of Netherton syndrome and application for prenatal testing, Br. J. Dermatol. 146 (2002) 495-499.
8. E. Bitoun, C. Bodemer, J. Amiel, Y. de Prost, C. Stoll, P. Calvas, A. Hovnanian, Prenatal diagnosis of a lethal form of Netherton syndrome by SPINK5 mutation analysis, Prenat. Diagn. 22 (2002) 121-126.
9. E. Bitoun, S. Chavanas, A.D. Irvine, L. Lonie, C. Bodemer, M. Paradisi, D. Hamel-Teillac, S. Ansai, Y. Mitsuhashi, A. Taieb, Y. de Prost, G. Zambruno, J.I. Harper, A. Hovnanian, Netherton syndrome: disease expression and spectrum of SPINK5 mutations in 21 families, J. Invest. Dermatol. 118 (2002) 352-361.
10. N. Komatsu, M. Takata, N. Otsuki, R. Ohka, O. Amano, K. Takehara, K. Saijoh, Elevated stratum corneum hydrolytic activity in Netherton syndrome suggests an inhibitory regulation of desquamation by SPINK5-derived peptides, J. Invest. Dermatol. 118 (2002) 436-443.
11. C. Stoll, Y. Alembik, D. Tchomakov, J. Messer, E. Heid, N. Boehm, P. Calvas, A. Hovnanian, Severe hypernatremic dehydration in an infant with Netherton syndrome, Genet. Counsel. 12 (2001) 237-243.
12. E. Sprecher, S. Chavanas, J.J. DiGiovanna, S. Amin, K. Nielsen, J.S. Prendiville, R. Silverman, N.B. Esterly, M.K. Spraker, E. Guelig, M.L. de Luna, M.L. Williams, B. Buehler, E.C. Siegfried, L. Van Maldergem, E. Pfendner, S.J. Bale, J. Uitto, A. Hovnanian, G. Richard, The spectrum of pathogenic mutations in SPINK5 in 19 families with Netherton syndrome: implications for mutation detection and first case of prenatal diagnosis, J. Invest. Dermatol. 117 (2001) 179-187.
13. H.E. Gonzalez, M. Gujrati, M. Frederick, Y. Henderson, A. Jayakumar, P.W. Spring, K. Mitsudo, H.W. Kim, G.L. Clayman, Identification of 9 genes differentially expressed in head and neck squamous cell carcinoma, Arch. Otolaryngol. Head Neck Surg. 129 (2003) 754-759.
14. K. Mitsudo, A. Jayakumar, Y. Henderson, M.J. Frederick, Y. Kang, M. Wang, A.K. El Naggar, G.L. Clayman, Inhibition of serine proteinases plasmin, trypsin, subtilisin A, cathepsin G, and elastase by LEKTI: a kinetic analysis, Biochemistry 42 (2003) 3874-3881.
15. A. Jayakumar, W.Y. Huang, B. Raetz, S.S. Chirala, S.J. Wakil, Cloning and expression of the multifunctional human fatty acid synthase and its subdomains in Escherichia coli, Proc. Natl. Acad. Sci. USA 93 (1996) 14509-14514.
16. A. Jayakumar, W. Epstein, E.M. Barnes Jr., Characterization of ammonium (methylammonium)/potassium antiport in Escherichia coli, J. Biol. Chem. 260 (1985) 7528-7532.
17. G.E. Smith, M.D. Summers, M.J. Fraser, Production of human beta interferon in insect cells infected with a baculovirus expression vector, Mol. Cell. Biol. 3 (1983) 2156-2165.
18. J. Cooley, B. Mathieu, E. Remold-O'Donnell, R.J. Mandle, Production of recombinant human monocyte/neutrophil elastase inhibitor (rM/NEI), Protein Exp. Purif. 14 (1998) 38-44.
19. G.A. Hastings, T.A. Coleman, C.C. Haudenschild, S. Stefansson, E.P. Smith, R. Barthlow, S. Cherry, M. Sandkvist, D.A. Lawrence, Neuroserpin, a brain-associated inhibitor of tissue plasminogen activator is localized primarily in neurons. Implications for the regulation of motor learning and neuronal survival, J. Biol. Chem. 272 (1997) 33062-33067.
20. V.A. Luckow, M.D. Summers, High level expression of nonfused foreign genes with Autographa californica nuclear polyhedrosis virus expression vectors, Virology 170 (1989) 31-39.
21. V.A. Luckow, M.D. Summers, Signals important for high-level expression of foreign genes in Autographa californica nuclear polyhedrosis virus expression vectors, Virology 167 (1988) 56-71.
22. A.M. Khatib, G. Siegfried, M. Chretien, P. Metrakos, N.G. Seidah, Proprotein convertases in tumor progression and malignancy: novel targets in cancer therapy, Am. J. Pathol. 160 (2002) 1921-1935.
23. A.M. Khatib, G. Siegfried, A. Prat, J. Luis, M. Chretien, P. Metrakos, N.G. Seidah, Inhibition of proprotein convertases is associated with loss of growth and tumorigenicity of HT-29 human colon carcinoma cells: importance of insulin-like growth factor-1 (IGF-1) receptor processing in IGF-1-mediated functions, J. Biol. Chem. 276 (2001) 30686-30693.
24. N. Komatsu, M. Takata, N. Otsuki, T. Toyama, R. Ohka, K. Takehara, K. Saijoh, Expression and localization of tissue kallikrein mRNAs in human epidermis and appendages, J. Invest. Dermatol. 121 (2003) 542-549.
25. F. Logeat, C. Bessia, C. Brou, O. LeBail, S. Jarriault, N.G. Seidah, A. Israel, The Notch1 receptor is cleaved constitutively by a furin-like convertase, Proc. Natl. Acad. Sci. USA 95 (1998) 8108-8112.
26. D.J. Pearton, W. Nirunsuksiri, A. Rehemtulla, S.P. Lewis, R.B. Presland, B.A. Dale, Proprotein convertase expression and localization in epidermis: evidence for multiple roles and substrates, Exp. Dermatol. 10 (2001) 193-203.
27. S.J. Duguay, W.M. Milewski, B.D. Young, K. Nakayama, D.F. Steiner, Processing of wild-type and mutant proinsulin-like growth factor-IA by subtilisin-related proprotein convertases, J. Biol. Chem. 272 (1997) 6663-6670.
28. A.J. Walley, S. Chavanas, M.F. Moffatt, R.M. Esnouf, B. Ubhi, R. Lawrence, K. Wong, G.R. Abecasis, E.Y. Jones, J.I. Harper, A. Hovnanian, W.O. Cookson, Gene polymorphism in Netherton and common atopic disease, Nat. Genet. 29 (2001) 175-178.
29. E. Bitoun, A. Micheloni, L. Lamant, C. Bonnart, A. Tartaglia-Polcini, C. Cobbold, T. Al Saati, F. Mariotti, J. Mazereeuw-Hautier, F. Boralevi, D. Hohl, J. Harper, C. Bodemer, M.D. Alessio, A. Hovnanian, LEKTI proteolytic processing in human primary keratinocytes tissue distribution and defective expression in Netherton syndrome, Hum. Mol. Genet. 2 (2003) 2417-2430.
30. M. Raghunath, L. Tontsidou, V. Oji, F. Schürmeyer-Horst, A. Jayakumar, G. Beljan, H. Ständer, J. Smolle, G.L. Clayman, H. Traupe, SPINK5 mutations: missing lekti and aberrant expression of Elafin, transglutaminases and human β-defensin 2 in Netherton Syndrome, J. Invest. Dermatol. (in press).