Ku86 exists as both a full-length and a protease-sensitive natural variant in multiple myeloma cells

Cancer Cell International

Volumn 8, Issue , 2008, Pages

  Gullo, Charles A ^a,b   Ge, Feng ^b   Cow, Geraline ^a,b   Teoh, Gerrard ^b,c  

^a SINGAPORE GENERAL HOSPITAL   (Singapore)

^b Singapore Health Services   (Singapore)

^c SGH   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOHYDRATE; NUCLEAR MATRIX PROTEIN; PROTEIN KU86; SERINE PROTEINASE;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL ISOLATION; CONTROLLED STUDY; ENZYME MODIFICATION; GENETIC VARIABILITY; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IN VITRO STUDY; LYMPHOCYTE; MULTIPLE MYELOMA; MYELOMA CELL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PROCESSING; WHOLE CELL;

EID: 43949086060     PISSN: None     EISSN: 14752867     Source Type: Journal    
DOI: 10.1186/1475-2867-8-4     Document Type: Article

References (34)

1. Nussenzweig A Chen C da CS V Sanchez M Sokol K Nussenzweig MC Li GC Requirement for Ku80 in growth and immunoglobulin V(D)J recombination Nature 1996, 382:551-555. 10.1038/382551a0 8700231
2. Lieber MR Grawunder U Wu X Yaneva M Tying loose ends: Roles of Ku and DNA-dependent protein kinase in the repair of double-strand breaks Curr Opin Genet Dev 1997, 7:99-104. 10.1016/S0959-437X(97)80116-5 9024627
3. Muller C Calsou P Frit P Cayrol C Carter T Salles B UV sensitivity and impaired nucleotide excision repair in DNA-dependent protein kinase mutant cells Nucleic Acids Res 1998, 26:1382-1389. 147427 9490781 10.1093/nar/26.6.1382
4. Salles B Calsou P Frit P Muller C The DNA repair complex DNA-PK, a pharmacological target in cancer chemotherapy and radiotherapy Pathol Biol (Paris) 2006, 54:185-193. 16563661
5. Tai YT Teoh G Lin B Davies FE Chauhan D Treon SP Raje N Hideshima T Shima Y Podar K Anderson KC Ku86 variant expression and function in multiple myeloma cells is associated with increased sensitivity to DNA damage J Immunol 2000, 165:6347-6355. 11086072
6. Tai YT Podar K Kraeft SK Wang F Young G Lin B Gupta D Chen LB Anderson KC Translocation of Ku86/Ku70 to the multiple myeloma cell membrane: functional implications Exp Hematol 2002, 30:212-220. 10.1016/ S0301-472X(01)00786-X 11882358
7. Gullo C Au M Feng G Teoh G The biology of Ku and its potential oncogenic role in cancer Biochim Biophys Acta 2006, 1756:223-234.
8. Tuteja R Tuteja N Ku autoantigen: A multifunctional DNA-binding protein Crit Rev Biochem Mol Biol 2000, 35:1-33. 10.1080/10409230091169177 10755664
9. Bakalkin G Yakovleva T Hurd YL Nussenzweig A Li GC Terenius L Autoantigen Ku in the brain. Developmentally regulated expression and subcellular localization Neuroreport 1998, 9:2147-2151. 10.1097/ 00001756-199806220-00044 9674610
10. Dalziel RG Mendelson SC Quinn JP The nuclear autoimmune antigen Ku is also present on the cell surface Autoimmunity 1992, 13:265-267. 10.3109/ 08916939209112334 1472635
11. Prabhakar BS Allaway GP Srinivasappa J Notkins AL Cell surface expression of the 70-kD component of Ku, a DNA-binding nuclear autoantigen J Clin Invest 1990, 86:1301-1305. 296862 2212014 10.1172/ JCI114838
12. Teoh G Urashima M Greenfield EA Nguyen KA Lee JF Chauhan D Ogata A Treon SP Anderson KC The 86-kD subunit of Ku autoantigen mediates homotypic and heterotypic adhesion of multiple myeloma cells J Clin Invest 1998, 101:1379-1388. 508693 9502780
13. Koike M Awaji T Kataoka M Tsujimoto G Kartasova T Koike A Shiomi T Differential subcellular localization of DNA-dependent protein kinase components Ku and DNA-PKcs during mitosis J Cell Sci 1999, 112 (Pt 22):4031-4039. 10547363
14. Koike M Ikuta T Miyasaka T Shiomi T Ku80 can translocate to the nucleus independent of the translocation of Ku70 using its own nuclear localization signal Oncogene 1999, 18:7495-7505. 10.1038/sj.onc.1203247 10602508
15. Tovari J Szende B Bocsi J Falaschi A Simoncsits A Pongor S Erchegyi J Stetak A Keri G A somatostatin analogue induces translocation of Ku 86 autoantigen from the cytosol to the nucleus in colon tumour cells Cell Signal 1998, 10:277-282. 10.1016/S0898-6568(97)00128-9 9617485
16. Muller C Dusseau C Calsou P Salles B Human normal peripheral blood B-lymphocytes are deficient in DNA-dependent protein kinase activity due to the expression of a variant form of the Ku86 protein Oncogene 1998, 16:1553-1560. 10.1038/sj.onc.1201676 9569022
17. Han Z Johnston C Reeves WH Carter T Wyche JH Hendrickson EA Characterization of a Ku86 variant protein that results in altered DNA binding and diminished DNA-dependent protein kinase activity J Biol Chem 1996, 271:14098-14104. 10.1074/jbc.271.24.14098 8662896
18. Jeng YW Chao HC Chiu CF Chou WG Senescent human fibroblasts have elevated Ku86 proteolytic cleavage activity Mutat Res 1999, 435:225-232. 10606813
19. Coffey G Campbell C An alternate form of Ku80 is required for DNA end-binding activity in mammalian mitochondria Nucleic Acids Res 2000, 28:3793-3800. 110772 11000272 10.1093/nar/28.19.3793
20. Lanuszewska J Widlak P The truncation of Ku86 in human lymphocytes Cancer Lett 2004, 205:197-205. 10.1016/j.canlet.2003.10.016 15036652
21. Sallmyr A Henriksson G Fukushima S Bredberg A Ku protein in human T and B lymphocytes: Full length functional form and signs of degradation Biochim Biophys Acta 2001, 1538:305-312. 10.1016/S0167-4889(01)00081-7 11336801
22. Sallmyr A Du L Bredberg A An inducible Ku86-degrading serine protease in human cells Biochim Biophys Acta 2002, 1593:57-68. 10.1016/ S0167-4889(02)00331-2 12431784
23. Hwang WY Gullo CA Shen J Poh CK Tham SC Cow G Au M Chan EW Teoh G Decoupling of normal CD40/interleukin-4 immunoglobulin heavy chain switch signal leads to genomic instability in SGH-MM5 and RPMI 8226 multiple myeloma cell lines Leukemia 2006, 20. 715-723. 10.1038/ sj.leu.2404099 16453006
24. Cai QQ Plet A Imbert J Lafage-Pochitaloff M Cerdan C Blanchard JM Chromosomal location and expression of the genes coding for Ku p70 and p80 in human cell lines and normal tissues Cytogenet Cell Genet 1994, 65:221-227. 8258294
25. Muller C Salles B Regulation of DNA-dependent protein kinase activity in leukemic cells Oncogene 1997, 15:2343-2348. 10.1038/sj.onc.1201402 9393878
26. Belessi C Stamatopolous K Kosmas C Glycosylation of V region genes in follicular lymphoma as a result of the somatic hypermutation mechanism Blood 2002, 100:2269-2270. 10.1182/blood-2002-05-1403 12229883
27. Farooq M Takahashi N Arrol H Drayson M Jefferis R Glycosylation of polyclonal and paraprotein IgG in multiple myeloma Glycoconj J 1997, 14:489-492. 10.1023/A:1018555619519 9249147
28. Nakamura F Kaimori M Takaya H Fujita K Suzuki N Sakurabayashi I Yoshioka N [Markedly elevated serum fructosamine in a non-diabetic patient with IgA-kappa type multiple myeloma] Rinsho Byori 1996, 44:85-89. 8691646
29. Gentzsch M Cui L Mengos A Chang XB Chen JH Riordan JR The PDZ-binding chloride channel ClC-3B localizes to the Golgi and associates with cystic fibrosis transmembrane conductance regulator-interacting PDZ proteins J Biol Chem 2003, 278:6440-6449. 10.1074/jbc.M211050200 12471024
30. Parham P Functions for MHC class I carbohydrates inside and outside the cell Trends Biochem Sci 1996, 21:427-433. 10.1016/S0968-0004(96)10053-0 8987398
31. van Leeuwen JE Kearse KP Deglucosylation of N-linked glycans is an important step in the dissociation of calreticulin-class I-TAP complexes Proc Natl Acad Sci U S A 1996, 93:13997-14001. 19483 8943049 10.1073/ pnas.93.24.13997
32. Mitsiades N Mitsiades CS Poulaki V Chauhan D Fanourakis G Gu X Bailey C Joseph M Libermann TA Treon SP Munshi NC Richardson PG Hideshima T Anderson KC Molecular sequelae of proteasome inhibition in human multiple myeloma cells Proc Natl Acad Sci U S A 2002, 99:14374-14379. 137891 12391322 10.1073/pnas.202445099
33. Quinn JP Simpson J Farina AR The Ku complex is modulated in response to viral infection and other cellular changes Biochim Biophys Acta 1992, 1131:181-187. 1319210
34. Wu X Lieber MR Protein-protein and protein-DNA interaction regions within the DNA end-binding protein Ku70-Ku86 Mol Cell Biol 1996, 16:5186-5193. 231519 8756676