Overexpression of calcineurin B subunit (CnB) enhances the oncogenic potential of HEK293 cells

Cancer Science

Volumn 99, Issue 6, 2008, Pages 1100-1108

  Wang, Yan Li ^a   Wang, Ying ^a   Tong, Li ^a   Wei, Qun ^a  

^a BEIJING NORMAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN CAPPING PROTEIN; CALCINEURIN; CALCINEURIN B; DJ 1 PROTEIN; F ACTIN CAPPING PROTEIN SUBUNIT ALPHA 1; GREEN FLUORESCENT PROTEIN; HEAT SHOCK PROTEIN; HEAT SHOCK PROTEIN 27; HEAT SHOCK PROTEIN BETA 1; HEAT SHOCK PROTEIN BETA 6; INDUCIBLE NITRIC OXIDE SYNTHASE; INOSITOL HEXAPHOSPHATE KINASE 1; MEMBRANE PROTEIN; MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN; PHOSPHOTRANSFERASE; PROTEIN; RETICULON 4 RECEPTOR LIKE 1; UBIQUITIN; UBIQUITIN LIKE DOMAIN CONTAINING PROTEIN 2;

AGAR MEDIUM; AMINO ACID SEQUENCE; ARTICLE; CARCINOGENESIS; CELL GROWTH; CELL PROLIFERATION; CELL TRANSFORMATION; CONTROLLED STUDY; EMBRYO; ENZYME ACTIVITY; GENE OVEREXPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; TIME OF FLIGHT MASS SPECTROMETRY; TWO DIMENSIONAL GEL ELECTROPHORESIS;

BLOTTING, WESTERN; CALCINEURIN; CELL MOVEMENT; CELL PROLIFERATION; CELL TRANSFORMATION, NEOPLASTIC; CELLS, CULTURED; COLONY-FORMING UNITS ASSAY; CULTURE MEDIA, SERUM-FREE; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HEAT-SHOCK PROTEINS; HUMANS; IMMUNOPRECIPITATION; KIDNEY; LIVER; ONCOGENE PROTEINS; PROTEIN SUBUNITS; RECOMBINANT FUSION PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 43549125834     PISSN: 13479032     EISSN: 13497006     Source Type: Journal    
DOI: 10.1111/j.1349-7006.2008.00799.x     Document Type: Article

References (43)

1. Klee CB, Crouch TH, Krinks MH. Calcineurin. a calcium- and calmodulin-binding protein of the nervous system. Proc Natl Acad Sci USA 1979; 76: 6270-3.
2. Klee CB, Ren H, Wang X. Regulation of the calmodulin stimulated protein phosphatase, calcineurin. J Biol Chem 1998; 273: 13367-70.
3. Wei Q, Zhang GF, Wang JY et al. Studies on immunological property and physiological function of calcineurin and its subunits. Chin J Chem 1993; 9: 240-2.
4. Wei Q, Lian ML, Jing FZ et al. Studies of calcineurin B subunit from genetic engineering for use in medicine. Drug Dev Res 2002; 56: 40-3.
5. Jin FZ, Lian ML, Wang X, Wei Q. Studies of the anticancer effect of calcineurin B. Immunopharmacol Immunotoxicol 2005; 27: 199-210.
6. Kahl CR, Means AR. Regulation of cell cycle progression by calcium/ calmodulin-dependent pathways. Endocr Rev 2003; 24: 719-36.
7. Xia L, Robert AS, Frank R, Diane LB. Inhibition of calcineurin phosphatase activity by a calcineurin B homologous protein. J Biol Chem 1999; 274: 36125-31.
8. Li W, Handschumacher RE. Identification of two calcineurin B-binding proteins: Tubulin and heat shock protein 60. Biochim Biophys Acta 2002; 1599: 72-81.
9. Saeki M, Irie Y, Ni L et al. Calcineurin potentiates the activation of procaspase-3 by accelerating its proteolytic maturation. J Biol Chem 2007; 282: 11786-94.
10. Jordan M, Wurm F. Transfection of adherent and suspended cells by calcium phosphate. Methods 2004; 33: 136-43.
11. Nakashima H, Hamamura K, Houjou T et al. Overexpression of caveolin-1 in a human melanoma cell line results in dispersion of ganglioside GD3 from lipid rafts and alteration of leading edges, leading to attenuation of malignant properties. Cancer Sci 2007; 98: 512-20.
12. Stoyan AR, Paul K, Chris B. The latent nuclear antigen of Kaposi sarcoma-associated herpesvirus targets the retinoblastoma-E2F pathway and with the oncogene Hras transforms primary rat cells. Nat Med 2000; 6: 1121-7.
13. Hu Y, Sun CY, Huang J, Hong L, Zhang L, Chu ZB. Antimyeloma effects of resveratrol through inhibition of angiogenesis. Chin Med J 2007; 120: 1672-7.
14. Fruman DA, Klee CB, Bierer BE, Burakoff SJ. Calcineurin phosphatase activity in T lymphocytes is inhibited by FK 506 and cyclosporin A. Proc Natl Acad Sci 1992; 89: 3686-90.
15. Qin YL, Yu DY, Wei Q. Function and structure of recombinant single chain calcineurin. Biochem Biophys Res Commun 2003; 308: 87-93.
16. Oliver R, Susanne H, Kai S et al. Calcineurin in human heart hypertrophy. Circulation 2002; 105: 2265-9.
17. Wang Z, Xie WQ, Chi F, Li CF. Identification of non-specific lipid transfer protein-1 as a calmodulin-binding protein in Arabidopsis. FEBS Lett 2005; 579: 1683-7.
18. Tian T, Hao J, Xu A et al. Determination of metastasis-associated proteins in non-small cell lung cancer by comparative proteomic analysis. Cancer Sci 2007; 98: 1265-74.
19. Michael WP. A new mathematical model for relative quantification in realtime RT-PCR. Nucleic Acids Res USA 2001;29: E45.
20. Hamburger AW, Salmon SE. Development of a bioassay for human myeloma colony-forming cells. Prog Clin Biol Res 1980; 48: 23-41.
21. Zhan X, Goldfarb M. Growth factor requirements of oncogene-transformed NIH 3T3 and BALB/c 3T3 cells cultured in defined media. Mol Cell Biol 1986; 6: 3541-4.
22. Lakshmikuttyamma A, Selvakumar P, Kanthan R, Kanthan SC, Sharma RK. Increased expression of calcineurin in human colorectal adenocarcinomas. J Cell Biochem 2005; 95: 731-9.
23. Huang CC, Wang JM, Kikkawa U et al. Calcineurin-mediated dephosphorylation of c-Jun Ser-243 is required for c-Jun protein stability and cell transformation. Oncogene 2008; in press.
24. Goto S, Matsukado Y, Mihara Y, Inoue N, Miyamoto E. An immunocytochemical demonstration of calcineurin in human nerve cell tumors. A comparison with neuron-specific enolase and glial fibrillary acidic protein. Cancer 1987; 60: 2948-57.
25. Jolly C, Morimoto RI. Role of the heat shock response and molecular chaperones in oncogenesis and cell death. J Natl Cancer Inst 2000; 92: 1564-72.
26. Ciocca DR, Oesterreich S, Chamness GCMC, Guire WL, Fugua SA. Biological and clinical implications of heat shock protein 27.000 (Hsp27): A review. J Natl Cancer Inst 1993; 85: 1558-70.
27. Clements CM, McNally RS, Conti BJ, Mak TW, Ting JP. DJ-1, a cancer- and Parkinson's disease-associated protein, stabilizes the antioxidant transcriptional master regulator Nrf2. Proc Natl Acad Sci USA 2006; 103: 15091-6.
28. Kim RH, Peters M, Jang Y et al. DJ-1, a novel regulator of the tumor suppressor PTEN. Cancer Cell 2005; 7: 263-73.
29. Le Naour F, Misek DE, Krause MC et al. Proteomics-based identification of RS/DJ-1 as a novel circulating tumor antigen in breast cancer. Clin Cancer Res 2001; 7: 3328-35.
30. Chen G, Gharib TG, Huang CC, Taylor JM, Misek DE. Discordant protein and mRNA expression in lung adenocarcinomas. Mol Cell Proteomics 2002; 1: 304-13.
31. Tian Q, Stepaniants SB, Mao M et al. Integrated genomic and proteomic analyses of gene expression in mammalian cells. Mol Cell Proteomics 2004; 3: 960-9.
32. Lucia T, Fulvio M, Cristina V. Characterization of heat shock protein 27 phosphorylation sites in renal cell carcinoma. Proteomics 2005; 5: 788-95.
33. Perroud B, Lee J, Valkova N. Pathway analysis of kidney cancer using proteomics and metabolic profiling. Mol Cancer 2006; 5: 64.
34. Boeckeler K, Tischendorf G, Mutzel R, Weissenmayer B. Aberrant stalk development and breakdown of tip dominance in Dictyostelium cell lines with RNAi-silenced expression of calcineurin B. BMC Dev Biol 2006; 2(6): 12.
35. Baek SJ, Kim KS, Nixon JB, Wilson LC, Eling TE. Cyclooxygenase inhibitors regulate the expression of a TGF-beta superfamily member that has proapoptotic and antitumorigenic activities. Mol Pharmacol 2001; 59: 901-8.
36. Numa F, Hirabayashi K, Tsunaga N et al. Elevated levels of syndecan-1 expression confer potent serum-dependent growth in human 293T cells. Cancer Res 1995; 55: 4676-80.
37. Steinbach WJ, Cramer RA Jr, Perfect BZ. Calcineurin controls growth, morphology, and pathogenicity in aspergillus fumigatus. Eukaryot Cell 2006; 5: 1091-103.
38. Sharpe GR, Fisher C. Time-dependent inhibition of growth of human keratinocytes and fibroblasts by cyclosporin A. effect on keratinocytes at therapeutic blood levels. Br J Dermatol 1990; 123: 207-13.
39. Thyberg J, Hansson GK. Cyclosporine A inhibits induction of DNA synthesis by PDGF and other peptide mitogens in cultured rat aortic smooth muscle cells and dermal fibroblasts. Growth Factors 1991; 4: 209-19.
40. Richter A, Davies DE, Alexander P. Growth inhibitory effects of FK506 and cyclosporin A independent of inhibition of calcineurin. Biochem Pharmacol 1995; 49: 367-73.
41. Tomono M, Toyoshima K, Ito M, Amano H. Calcineurin is essential for DNA synthesis in Swiss 3T3 fibroblasts. Biochem J 1996; 317: 675-80.
42. Mustafa AS, Shaban FA. ProPred analysis and experimental evaluation of promiscuous T-cell epitopes of three major secreted antigens of Mycobacterium tuberculosis. Tuberculosis (Edinb) 2006; 86: 115-24.
43. Barrett JC. Mechanisms of multistep carcinogenesis and carcinogen risk assessment. Environ Health Perspect 1993; 100: 9-20.