Assays for G-protein-coupled receptor signaling using RGS-insensitive Gα subunits

Methods in Enzymology

Volumn 389, Issue , 2004, Pages 155-169

  Clark, Mary J ^a   Traynor, John R ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENYLATE CYCLASE; G PROTEIN COUPLED RECEPTOR; MITOGEN ACTIVATED PROTEIN KINASE 1; PERTUSSIS TOXIN; PROTEIN SUBUNIT; RGS PROTEIN;

ALPHA CHAIN; ANIMAL CELL; CALCIUM CELL LEVEL; CONTROLLED STUDY; ENZYME PHOSPHORYLATION; GLIOMA CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DETERMINATION; PROTEIN EXPRESSION; RAT; REVIEW;

ANIMALIA; MAMMALIA;

EID: 4344691146     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(04)89010-4     Document Type: Article

References (22)

1. 35S]thio)triphosphate binding in digitonin-permeabilized C6 rat glioma cells: Evidence for an organized association of μ-opioid receptors and G protein. J. Pharmacol. Exp. Ther. 298:2001;116-121
2. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254
3. 1α inhibits the calcium dependence of β-adrenergic stimulated cAMP accumulation in C6 glioma cells. J. Biol. Chem. 268:1993;8980-8989
4. o: Effects on adenylyl cyclase, extracellular signal-regulated kinases and intracellular calcium pathways. J. Biol. Chem. 278:2003;9418-9425
5. Doupnik C.A., Jaén C., Zhang Q. Measuring the modulating effects of RGS proteins on GIRK channel gating. Methods Enzymol. 389:(9):2004;131-154
6. Gutstein H.B., Rubie E.A., Mansour A., Akil H., Woodgett J.R. Opioid effects on mitogen-activated protein kinase signaling cascades. Anesthesiology. 87:1997;1118-1126
7. 35S]GTPγS binding assay: Approaches and applications in pharmacology. Life Sci. 74:2003;489-508
8. Hollinger S., Hepler J.R. Cellular regulation of RGS proteins: Modulators and integrators of G protein signaling. Pharmacol. Rev. 54:2002;527-559
9. Kenakin T. Drug efficacy at G protein-coupled receptors. Annu. Rev. Pharmacol. Toxicol. 42:2002;349-379
10. il blocks interaction with regulator of G protein signaling proteins. J. Biol. Chem. 21:1998;12794-12797
11. Law P.Y., Wong Y.H., Loh H.H. Molecular mechanisms and regulation of opioid receptor signaling. Annu. Rev. Pharmacol. Toxicol. 40:2000;389-430
12. Lee K.O., Akil H., Woods J.H., Traynor J.R. Differential binding properties of oripavines at cloned μ- and δ-opioid receptors. Eur. J. Pharmacol. 378:1999;323-330
13. Milligan G. Techniques used in the identification and analysis of function of pertussis toxin-sensitive guanine nucleotide binding proteins. Biochem. J. 255:1998;1-13
14. Neubig R.R., Siderovski D.P. Regulators of G-protein signaling as new central nervous system drug targets. Nature Rev. Drug Disc. 1:2002;187-197
15. Smart D., Lambert D.G. The stimulatory effects of opioids and their possible role in the development of tolerance. Trends Pharmacol. Sci. 17:1996;264-269
16. Snow B.E., Hall R.A., Krumins A.M., Brothers G.M., Bouchard D., Brothers C.A., Chung S., Mangion J., Gilman A.G., Lefkowitz R.J., Siderovski D.P. GTPase activating specificity of RGS12 and binding specificity of an alternatively spliced PDZ (PSD-95DlgZO-1) domain. J. Biol. Chem. 10:1998;17749-17755
17. iα1: Stabilization of the transition state for GTP hydrolysis. Cell. 89:1997;251-261
18. Wang Q., Liu M., Bashar M., Siderovski D.P., Neubig R.R. Receptor-selective effects of endogenous RGS3 and RGS5 to regulate mitogen-activated protein kinase activation in rat vascular smooth muscle cells. J. Biol. Chem. 277:2002;24949-24958
19. Watts V.J. Molecular mechanisms for heterologous sensitization of adenylate cyclase. J. Pharmacol. Exp. Ther. 302:2002;1-7
20. 35S]GTPγS binding. Methods Enzymol. 344:2002;3-14
21. Zeng W., Xu X., Popov S., Mukhopadhyay S., Chidiac P., Swistok P., Danho W., Yagaloff K.A., Fisher S.L., Ross E.M., Muallem S., Wilkie T.M. The N-terminal domain of RGS4 confers receptor-selective inhibition of G protein signaling. J. Biol. Chem. 273:1998;34687-34690
22. Zhong H., Wade S.M., Woolf P.J., Linderman J.J., Traynor J.R., Neubig R.R. A spatial focusing model for G protein signals: Regulators of G protein signaling (RGS) protein mediated kinetic scaffolding. J. Biol. Chem. 278:2003;7278-7284