RGS-insensitive G-protein mutations to study the role of endogenous RGS proteins

Methods in Enzymology

Volumn 389, Issue , 2004, Pages 229-243

  Fu, Ying ^a   Zhong, Huailing ^a   Nanamori, Masakatsu ^a   Mortensen, Richard M ^b   Huang, Xinyan ^a   Lan, Kengli ^a   Neubig, Richard R ^b  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF MICHIGAN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE RECEPTOR STIMULATING AGENT; ADENYLATE CYCLASE; DNA; ENKEPHALIN[2 DEXTRO ALANINE 4 METHYLPHENYLALANINE 5 GLYCINE]; GLYCINE; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE TRIPHOSPHATASE ACTIVATING PROTEIN; ISOPRENALINE; MESSENGER RNA; MITOGEN ACTIVATED PROTEIN KINASE; MORPHINE; MU OPIATE RECEPTOR; NEOMYCIN; NORADRENALIN; RETINOIC ACID; RGS PROTEIN; RNA; SERINE;

ALPHA CHAIN; AMINO ACID SEQUENCE; BETA CHAIN; BRADYCARDIA; CALCIUM CURRENT; CELL LINE; CELL MUTANT; CONCENTRATION RESPONSE; CULTURE MEDIUM; ELECTROPORATION; EMBRYO CELL; ENZYME ACTIVITY; EXON; GENE DELETION; GENE MUTATION; GENETIC TRANSFECTION; GLIOMA CELL; HEART BEAT; HEART MUSCLE CELL; NERVE CELL; NERVE CELL DIFFERENTIATION; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; REVIEW; RNA EXTRACTION; SIGNAL TRANSDUCTION; SOUTHERN BLOTTING; STATISTICAL SIGNIFICANCE; WILD TYPE; YEAST;

EID: 4344670031     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(04)89014-1     Document Type: Article

References (35)

1. Berman D.M., Kozasa T., Gilman A.G. The GTPase-activating protein RGS4 stabilizes the transition state for nucleotide hydrolysis. J. Biol. Chem. 271:(44):1996;27209-27212
2. Boheler K.R., Czyz J., Tweedie D., Yang H.T., Anisimov S.V., Wobus A.M. Differentiation of pluripotent embryonic stem cells into cardiomyocytes. Circ. Res. 91:(3):2002;189-201
3. Chan R.K., Otte C.A. Isolation and genetic analysis of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and alpha factor pheromones. Mol. Cell Biol. 2:(1):1982;11-20
4. Chen H., Lambert N.A. Endogenous regulators of G protein signaling proteins regulate presynaptic inhibition at rat hippocampal synapses. Proc. Natl. Acad. Sci. USA. 97:(23):2000;12810-12815
5. Clark M.J., Harrison C., Zhong H., Neubig R.R., Traynor J.R. Endogenous RGS protein action modulates mu-opioid signaling through Gα: Effects on adenylyl cyclase, extracellular signal-regulated kinases, and intracellular calcium pathways. J. Biol. Chem. 278:(11):2003;9418-9425
6. DiBello P.R., Garrison T.R., Apanovitch D.M., Hoffman G., Shuey D.J., Mason K., Cockett M.I., Dohlman H.G. Selective uncoupling of RGS action by a single point mutation in the G protein alpha-subunit. J. Biol. Chem. 273:(10):1998;5780-5784
7. Dohlman H.G., Apaniesk D., Chen Y., Song J., Nusskern D. Inhibition of G-protein signaling by dominant gain-of-function mutations in Sst2p, a pheromone desensitization factor in Saccharomyces cerevisiae. Mol. Cell Biol. 15:(7):1995;3635-3643
8. Gu H., Zou Y.R., Rajewsky K. Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting. Cell. 73:1993;1155-1164
9. Hepler J.R., Berman D.M., Gilman A.G., Kozasa T. RGS4 and GAIP are GTPase-activating proteins for Gq alpha and block activation of phospholipase C beta by gamma-thio-GTP-Gq alpha. Proc. Natl. Acad. Sci. USA. 94:(2):1997;428-432
10. Hescheler J., Fleischmann B.K., Lentini S., Maltsev V.A., Rohwedel J., Wobus A.M., Addicks K. Embryonic stem cells: A model to study structural and functional properties in cardiomyogenesis. Cardiovasc. Res. 36:(2):1997;149-162
11. Heximer S.P., Knutsen R.H., Sun X., Kaltenbronn K.M., Rhee M.H., Peng N., Oliveira-dos-Santos A., Penninger J.M., Muslin A.J., Steinberg T.H., Wyss J.M., Mecham R.P., Blumer K.J. Hypertension and prolonged vasoconstrictor signaling in RGS2-deficient mice. J. Clin. Invest. 111:(4):2003;445-452
12. Hollinger S., Hepler J.R. Cellular regulation of RGS proteins: Modulators and integrators of G protein signaling. Pharmacol Rev. 54:(3):2002;527-559
13. Hunt T.W., Fields T.A., Casey P.J., Peralta E.G. RGS10 is a selective activator of G alpha i GTPase activity. Nature. 383:(6596):1996;175-177
14. Jeong S.W., Ikeda S.R. Endogenous regulator of G-protein signaling proteins modify N-type calcium channel modulation in rat sympathetic neurons. J. Neurosci. 20:(12):2000;4489-4496
15. Lan K.L. "Mechanism and Specificity of G Protein Regulation by RGS Proteins." 1999;. Dissertation, University of Michigan.
16. i1 blocks interaction with regulators of G protein signaling. J. Biol. Chem. 273:1998;12794-12797
17. Laugwitz K.L., Offermanns S., Spicher K., Schultz G. Mu and delta opioid receptors differentially couple to G protein subtypes in membranes of human neuroblastoma SH-SY5Y cells. Neuron. 10:(2):1993;233-242
18. 1A receptors. Biochemistry. 40:2001;10532-10541
19. Martemyanov K.A., Hopp J.A., Arshavsky V.Y. Specificity of G protein-RGS protein recognition is regulated by affinity adapters. Neuron. 38:(6):2003;857-862
20. Metzger J.M., Lin W.I., Samuelson L.C. Transition in cardiac contractile sensitivity to calcium during the in vitro differentiation of mouse embryonic stem cells. J. Cell Biol. 126:(3):1994;701-711
21. Milligan G. Techniques used in the identification and analysis of function of pertussis toxin-sensitive guanine nucleotide binding proteins. Biochem. J. 255:(1):1988;1-13
22. Mortensen R.M., Seidman J.G. Inactivation of G-protein genes: Double knockout in cell lines. Methods Enzymol. 237:1994;356-366
23. Natochin M., Artemyev N.O. A single mutation Asp229-Ser confers upon Gsα the ability to interact with regulators of G protein signaling. Biochemistry. 37:1998;13776-13780
24. Neubig R.R., Siderovski D.P. Regulators of G-protein signaling as new central nervous system drug targets. Nature Rev. Drug. Discov. 1:(3):2002;187-197
25. Oliveira-dos-Santos A.J., Matsumoto G., Snow B.E., Bai D., Houston F.P., Whishaw I.Q., Mariathasan S., Sasaki T., Wakeham A., Ohashi P.S., Roder J.C., Barnes C.A., Siderovski D.P., Penninger J.M. Regulation of T cell activation, anxiety, and male aggression by RGS2. Proc. Natl. Acad. Sci. USA. 97:(22):2000;12272-12277
26. Rahman Z., Schwarz J., Gold S.J., Zachariou V., Wein M.N., Choi K.H., Kovoor A., Chen C.K., DiLeone R.J., Schwarz S.C., Selley D.E., Sim-Selley L.J., Barrot M., Luedtke R.R., Self D., Neve R.L., Lester H.A., Simon M.I., Nestler E.J. RGS9 modulates dopamine signaling in the basal ganglia. Neuron. 38:(6):2003;941-952
27. Ross E.M., Wilkie T.M. GTPase-activating proteins for heterotrimeric G proteins: Regulators of G protein signaling (RGS) and RGS-like proteins. Annu. Rev. Biochem. 69:2000;795-827
28. Sachinidis A., Fleischmann B.K., Kolossov E., Wartenberg M., Sauer H., Hescheler J. Cardiac specific differentiation of mouse embryonic stem cells. Cardiovasc. Res. 58:(2):2003;278-291
29. i1α and βγ binding. J. Biol. Chem. 73:(14):1998;7934-7940
30. Sternweis P.C., Robishaw J.D. Isolation of two proteins with high affinity for guanine nucleotides from membranes of bovine brain. J. Biol. Chem. 259:(22):1984;13806-13813
31. Strittmatter S.M., Valenzuela D., Kennedy T.E., Neer E.J., Fishman M.C. Go is a major growth cone protein subject to regulation by GAP-43. Nature. 344:(6269):1990;836-841
32. Tang M., Wang G., Lu P., Karas R.H., Aronovitz M., Heximer S.P., Kaltenbronn K.M., Blumer K.J., Siderovski D.P., Zhu Y., Mendelsohn M.E. Regulator of G-protein signaling-2 mediates vascular smooth muscle relaxation and blood pressure. Nature Med. 9:(12):2003;1506-1512
33. Tesmer J.J., Berman D.M., Gilman A.G., Sprang S.R. Structure of RGS4 bound to AlF4-activated G(i alpha1): Stabilization of the transition state for GTP hydrolysis. Cell. 89:(2):1997;251-261
34. Ye C., Sowell M.O., Vassilev P.M., Milstone D.S., Mortensen R.M. Galpha(i2), alpha(i3) and Galpha(o) are all required for normal muscarinic inhibition of the cardiac calcium channels in nodalatrial-like cultured cardiocytes. J. Mol. Cell Cardiol. 31:(9):1999;1771-1781
35. Zhong H., Neubig R.R. Regulator of G protein signaling proteins: Novel multifunctional drug targets. J. Pharmacol. Exp. Ther. 297:(3):2001;837-845