Softening of the actin cytoskeleton by inhibition of myosin II

Pflugers Archiv European Journal of Physiology

Volumn 456, Issue 1, 2008, Pages 95-100

  Martens, Jan Christian ^a   Radmacher, Manfred ^a  

^a UNIVERSITY OF BREMEN   (Germany)

Author keywords

Actin; Cytoskeleton; Fibroblast; Mechanical properties; Myosin

Indexed keywords

4 (1 AMINOETHYL) N (4 PYRIDYL)CYCLOHEXANECARBOXAMIDE; ACTIN; BLEBBISTATIN; MYOSIN II;

ACTIN FILAMENT; ANIMAL CELL; ARTICLE; ATOMIC FORCE MICROSCOPY; CONTROLLED STUDY; CYTOSKELETON; FIBROBLAST; IMAGE QUALITY; MICROSCOPE IMAGE; NONHUMAN; PRIORITY JOURNAL; PROTEIN FUNCTION; RAT; YOUNG MODULUS;

ACTINS; AMIDES; ANIMALS; BIOMECHANICS; CELL LINE; CELL SHAPE; CYTOSKELETON; ELASTICITY; ENZYME INHIBITORS; FIBROBLASTS; HETEROCYCLIC COMPOUNDS WITH 4 OR MORE RINGS; MICROSCOPY, ATOMIC FORCE; MYOSIN TYPE II; PYRIDINES; RATS; RHO-ASSOCIATED KINASES;

EID: 43149101492     PISSN: 00316768     EISSN: 14322013     Source Type: Journal    
DOI: 10.1007/s00424-007-0419-8     Document Type: Article

References (29)

1. Domke J, Radmacher M (1998) Measuring the elastic properties of thin polymer films with the AFM. Langmuir 14(12):3320-3325
2. Duszyk M, Schwab B III, Zahalak GI, Qian H, Elson EL (1989) Cell poking: quantitative analysis of indentation of thick viscoelastic layers. Biophys J 55:683-690
3. Elson EL (1988) Cellular mechanics as an indicator of cytoskeletal structure and function. Annu Rev Biophys Biophys Chem 17:397-430
4. Fung YC (1993) Biomechanics-mechanical properties of living tissues. Springer, Berlin Heidelberg New York
5. Guo W-h, Frey MT, Burnham NA, Wang Y-l (2006) Substrate rigidity regulates the formation and maintenance of tissues. Biophys J 90:2213-2220
6. Hertz H (1882) Über die Berührung fester elastischer Körper. J Reine Angew Math 92:156-171
7. Kasas S, Wang X, Hirling H, Marsault R, Huni B, Yersin A, Regazzi R, Greeningloh G, Riederer B, Forrò L, Dietler G, Catsicas S (2005) Superficial and deep changes of cellular mechanical properties following cytoskeleton disassembly. Cell Motil Cytoskeleton 62:124-132
8. Limouze J, Straight A, Mitchison T, Sellers J (2004) Specificity of blebbistatin, an inhibitor of myosin II. J Muscle Res Cell Motil 25(4):337-341
9. Mahaffy RE, Park S, Gerde E, Käs J, Shih CK (2004) Quantitative analysis of the viscoelastic properties of thin regions of fibroblasts using atomic force microscopy. Biophys J 86:1777-1793
10. Masayoshi U, Toshimasa I, Hiroyuki S, Takashi O, Toshio K, Tamami M, Hiroki T, Keiji Y, Jun I, Midori M, Shuh N (1997) Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension. Nature 389:990-994
11. Matzke R, Jacobson K, Radmacher M (2001) Direct, high resolution measurement of furrow stiffening during the division of adherent cells. Nat Cell Biol 3:607-610
12. McElfresh M, Baesu E, Balhorn R, Belak J, Allen MJ, Rudd RE (2002) Combining constitutive materials modeling with atomic force microscopy to understand the mechanical properties of living cells. Proc Natl Acad Sci U S A 99:6493-6497
13. Nakayama M, Amano M, Katsumi A, Kaneko T, Kawabata S, Takefuji M, Kaibuchi K (2005) Rho-kinase and myosin II activities are required for cell type and environment specific migration. Genes Cells 10(2):107-117
14. Ogilvy JA (1993) A parametric elastic model for indentation testing of thin films. J Phys D Appl Phys 26:2123-2131
15. Prass M, Jacobson K, Mogilner A, Radmacher M (2006) Direct measurement of the lamellipodial protrusive force in a migrating cell. J Cell Biol 174(6):767-772
16. Radmacher M (2007) Studying the mechanics of cellular processes by atomic force microscopy. In: Wang Y, Discher DE (eds) Cell mechanics. Academic, New York, pp 347-372
17. Radmacher M (2007) Studying the mechanics of cellular processes by atomic force microscopy. Methods Cell Biol 83:347-372
18. Radmacher M, Cleveland JP, Fritz M, Hansma HG, Hansma PK (1994) Mapping interaction forces with the atomic force microscope. Biophys J 66(6):2159-2165
19. Radmacher M, Fritz M, Kacher CM, Cleveland JP, Hansma PK (1996) Measuring the elastic properties of human platelets with the atomic force microscope. Biophys J 70(1):556-567
20. Rico F, Roca-Cusachs P, Gavara N, Farrè R, Rotger M, Navajas D (2005) Probing mechanical properties of living cells by atomic force microscopy with blunted pyramidal cantilever tips. Phys Rev E 72:021914
21. Rotsch C, Jacobson K, Radmacher M (1999) Dimensional and mechanical dynamics of active and stable edges in motile fibroblasts investigated by atomic force microscopy. Proc Natl Acad Sci U S A 96:921-926
22. Rotsch C, Radmacher M (2000) Drug-induced changes of cytoskeletal structure and mechanics in fibroblasts-an atomic force microscopy study. Biophys J 78:520-535
23. Saitoh M, Ishikawa T, Matsushima S, Naka M, Hidaka H (1987) Selective inhibition of catalytic activity of smooth muscle myosin light chain kinase. JBC 262(16):7796-7801
24. Sakamoto T, Limouze J, Combs CA, Straight AF, Sellers JR (2005) Blebbistatin, a myosin II inhibitor, is photoinactivated by blue light. Biochemistry 44(2):584-588
25. Schäfer A, Radmacher M (2005) Influence of myosin II activity on stiffness of fibroblast cells. Acta Biomater 1:273-280
26. Sneddon IN (1965) The relation between load and penetration in the axisymmetric Boussinesq problem for a punch of arbitrary profile. Int J Eng Sci 3:47-57
27. Straight A, Cheung A, Limouze J, Chen I, Westwood N, Sellers J, Mitchison T (2003) Dissecting temporal and spatial control of cytokinesis with a myosin II Inhibitor. Science 299(5613):1743-1747
28. Totsukawa G, Wu Y, Sasaki Y, Hartshorne DJ, Yamakita Y, Yamashiro S, Matsumura F (2004) Distinct roles of MLCK and ROCK in the regulation of membrane protrusins and focal adhesion dynamics during cell migration of fibroblasts. J Cell Biol 164(3):427-439
29. Totsukawa G, Yamakita Y, Yamashiro S, Hartshorne DJ, Sasaki Y, Matsumura F (2000) Distinct roles of ROCK (rho-kinase) and MLCK in spatial regulation of MLC phosphorylation for assembly of stress and focal adhesion in 3T3 fibroblasts. J Cell Biol 150:797-806