Blebbistatin, a myosin II inhibitor, is photoinactivated by blue light

Biochemistry

Volumn 44, Issue 2, 2005, Pages 584-588

  Sakamoto, Takeshi ^a   Limouze, John ^a   Combs, Christian A ^a   Straight, Aaron F ^b   Sellers, James R ^a  

^a NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

^b STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOASSAY; CELLS; ENZYMES; FREE RADICALS; PHOTOCHEMICAL REACTIONS;

BLEBBISTATIN; BLUE LIGHT; MGATPASE ACTIVITY; VITRO MOTILITY;

PROTEINS;

BLEBBISTATIN; MYOSIN 2 INHIBITOR; MYOSIN II; PROTEIN INHIBITOR; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; BLUE LIGHT; CELL DAMAGE; CELL KINETICS; CELL MOTILITY; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL;

ACTINS; ANIMALS; DOSE-RESPONSE RELATIONSHIP, RADIATION; FREE RADICALS; HELA CELLS; HETEROCYCLIC COMPOUNDS WITH 4 OR MORE RINGS; HUMANS; LIGHT; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; MITOSIS; MUSCLE CONTRACTION; MUSCLE, SKELETAL; NONMUSCLE MYOSIN TYPE IIA; PHOTOCHEMISTRY; RABBITS; VIDEOTAPE RECORDING;

ANIMALIA;

EID: 12144265571     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0483357     Document Type: Article

References (14)

1. Berg, J. S., Powell, B. C., and Cheney, R. E. (2001) A millennial myosin census. Mol. Biol. Cell 12, 780-194.
2. Kieke, M. C., and Titus, M. A. (2003) The myosin superfamily: An overview, in Molecular Motors (Schliwa, M., Ed.) pp 3-44, Wiley-VCH, Weinheim, Germany.
3. Straight, A. F., Cheung, A., Limouze, J., Chen, I., Westwood, N. J., Sellers, J. R., and Mitchison, T. J. (2003) Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor, Science 299, 1743-1747.
4. Limouze, J., Straight, A. F., Mitchison, T. J., and Sellers, J. R. (2004) Specificity of blebbistatin, a myosin II inhibitor, J. Muscle Res. Cell Motil. 25, 337-341.
5. Spudich, J. A., and Watt, S. (1971) The regulation of rabbit skeletal muscle contraction, J. Biol. Chem. 246, 4866-4871.
6. Margossian, S. S., and Lowey, S. (1982) Preparation of myosin and its subfragments from rabbit skeletal muscle, Methods Enzymol. 85, 55-71.
7. Toyoshima, Y. Y., Kron, S. J., McNally, E. M., Niebling, K. R., Toyoshima, C., and Spudich, J. A. (1987) Myosin subfragment-1 is sufficient to move actin filaments in vitro, Nature 328, 536-539.
8. Sellers, J. R., Cuda, G., Wang, F., and Homsher, E. (1993) Myosin-Specific Adaprations of Motility Assays, in Motility Assays for Motor Proteins (Scholey, J. M., Ed.) pp 23-49, Academic Press, San Diego.
9. Sakamoro, T., Wang, F., Schmitz, S., Xu, Y. H., Xu, Q., Molloy, J. E., Veigel, C., and Sellers, J. R. (2003) Neck length and processivity of myosin V, J. Biol. Chem. 278, 29201-29207.
10. Cogswell, C. J., and Larkin, K. G. (1989) The specimen illumination path and its effect on image quality, in The Handbook of Confocal Microscopy (Pauley, J., Ed.) pp 127-137, Plenum Press, New York.
11. Cuda, G., Pare, E., Cooke, R., and Sellers, J. R. (1997) In virro actin filament sliding velocities produced by mixtures of different types of myosin, Biophys. J. 72, 1767-1779.
12. Kovacs, M., Toth, J., Hetenyi, C., Malnasi-Csizmadia, A., and Sellers, J. R. (2004) Mechanism of blebbistatin inhibition of myosin II. J. Biol. Chem. 279, 35557-35563.
13. Kolega, J. (2004) Phototoxicity and photoinactivation of blebbistatin in UV and visible light. Biochem. Biophys. Res. Commun. 320, 1020-1025.
14. Rosenblatt, J., Cramer, L. P., Baum, B., and McGee, K. M. (2004) Myosin II-dependent cortical movement is required for centrosome separation and positioning during mitotic spindle assembly, Cell 117, 361-372.