Cloning and expression of a fibrinolytic enzyme (subtilisin DFE) gene from Bacillus amyloliquefaciens DC-4 in Bacillus subtilis

Research in Microbiology

Volumn 155, Issue 3, 2004, Pages 167-173

  Peng, Yong ^a,b   Yang, Xiao Juan ^a   Xiao, Lu ^a   Zhang, Yi Zheng ^a  

^a SICHUAN UNIVERSITY   (China)

^b OHIO STATE UNIVERSITY   (United States)

Author keywords

Bacillus amyloliquefaciens; Bacillus subtilis; Fibrinolytic enzyme; Nattokinase; Subtilisin DFE

Indexed keywords

BACTERIAL ENZYME; DOUCHI FIBRINOLYTIC ENZYME; PLASMIN; SERINE PROTEINASE; SUBTILISIN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BACILLUS AMYLOLIQUEFACIENS; BACILLUS SUBTILIS; BACTERIAL GENE; ENZYME ISOLATION; ESCHERICHIA COLI; EXPRESSION VECTOR; FERMENTED PRODUCT; GENE AMPLIFICATION; GENE EXPRESSION; GENETIC CODE; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; SHUTTLE VECTOR; SOYBEAN; SUBTILISIN BPN';

AMINO ACID SEQUENCE; BACILLUS; BASE SEQUENCE; CLONING, MOLECULAR; DNA, BACTERIAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FIBRIN; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SUBTILISINS;

BACILLUS AMYLOLIQUEFACIENS; BACILLUS SUBTILIS; ESCHERICHIA COLI; GLYCINE MAX; VECTORS;

EID: 1842418551     PISSN: 09232508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.resmic.2003.10.004     Document Type: Article

References (21)

1. Astrup T., Mullertz S. The fibrin plate method for estimating fibrinolytic activity. Arch. Biochem. Biophys. 40:1952;346-351.
2. Brunner G., Schirrmacher V. Fibrin autograghy of plasminogen activator by electrophoretic transfer into fibrin agar gels. Anal. Biochem. 168:1988;411-416.
3. Fujita M., Nomura K., Hong K., Ito Y., Asada A., Nishimuro S. Purification and characterization of a strong fibrinolytic enzyme (nattokinase) in the vegetable cheese natto, a popular soybean-fermented food in Japan. Biochem. Biophys. Res. Commun. 197:1993;1340-1347.
4. Inoue H., Nojima H., Okayama H. High efficiency transformation of Escherichia coli with plasmids. Gene. 96:1990;23-28.
5. Kim S.H., Choi N.S. Purification and characterization of subtilisin DJ-4 secreted by Bacillus sp. strain DJ-4 screened from Doen-Jang. Biosci. Biotechnol. Biochem. 64:2000;1722-1725.
6. Kim W., Choi K., Kim Y., Park H., Choi J., Lee Y., Oh H., Kwon I., Lee S. Purification and characterization of a fibrinolytic enzyme produced from Bacillus sp. strain CK 11-4 screened from Chungkook-Jang. Appl. Environ. Microbiol. 62:1996;2482-2488.
7. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
8. Liu C.J., Huang Q., Zhao L., Zhang Y.Z. Construction of a Escherichia coli-Bacillus subtilis shuttle vector pSUGV4. J. Sichuan Univ. (Natural Science Edition). 38:2001;243-246.
9. Nakamura T., Yamagata Y., Ichishima E. Nucleotide sequence of the subtilisin NAT, aprN, of Bacillus subtilis (natto). Biosci. Biotechnol. Biochem. 56:1992;1869-1871.
10. Peng Y., Huang Q., Zhang R.H., Zhang Y.Z. Purification and characterization of a fibrinolytic enzyme produced by Bacillus amyloliquefaciens DC-4 screened from douchi, a traditional Chinese soybean food. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 134:2003;45-52.
11. Peng Y., Zhang Y.Z. Isolation and characterization of fibrinolytic enzyme-producing strain DC-4 from Chinese douchi and primary analysis of the enzyme property. High Technol. Lett. 12:(2):2002;30-33.
12. Sadaie Y., Kada T. Formation of competent Bacillus subtilis cells. J. Bacteriol. 153:1983;813-821.
13. Saito H., Miura K. Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochem. Biophys. Acta. 72:1963;619-629.
14. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual. second ed. 1989;Cold Spring Harbor Laboratory Press, New York.
15. Saris P., Taira S., Airaksinen U., Palva A., Sarvas M., Palva I., Runeberg-Nyman K. Production and secretion of pertussis toxin subunits in Bacillus subtilis. FEMS Microbiol. Lett. 56:1990;143-148.
16. Sumi H., Hamada H., Mihara H., Nakanishi K., Hiratani H. Fibrinolytic effect of the Japanese traditional food natto (nattokinase). Thrombo. Haemostasis. 62:1989;549.
17. Sumi H., Hamada H., Nakanishi K. Enhancement of the fibrinolytic activity in plasma by oral administration of nattokinase. Acta Haematol. 84:1990;139-143.
18. Sumi H., Hamada H., Tsushima H. A novel fibrinolytic enzyme (nattokinase) in the vegetable cheese Natto: A typical and popular soybean food in the Japanese diet. Experientia. 43:1987;1110-1111.
19. Sumi H., Nakajima N., Yatagai C. A unique strong fibrinolytic enzyme (Katsuwokinase) in skipjack "shiokara", a Japanese traditional fermented food. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 112:1995;543-547.
20. Vasantha N., Thompson L.D., Rhodes C., Banner C.D.B., Nagle J., Filpula D. Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein. J. Bacteriol. 159:1984;811-819.
21. Wu X.C., Lee W., Tran L., Wong S.L. Engineering a Bacillus subtilis expression-secretion system synthesis with a strain deficient in six extracellular proteases. J. Bacteriol. 173:1991;4952-4958.