Gene cloning and expression of an alkaline serine protease with dehairing function from Bacillus pumilus

Current Microbiology

Volumn 49, Issue 3, 2004, Pages 165-169

  Pan, Jiao ^a   Huang, Qing ^a   Zhang, YiZheng ^a  

^a SICHUAN UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE SERINE PROTEASE; BACTERIAL ENZYME; GENE PRODUCT; PLASMID VECTOR; SERINE PROTEINASE; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS PUMILUS; BACTERIAL GENE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE CONSTRUCT; GENE EXPRESSION; GENE IDENTIFICATION; LEATHER INDUSTRY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION;

BACILLUS PUMILUS; BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; POSIBACTERIA; PROKARYOTA; PUMILUS;

EID: 4344635429     PISSN: 03438651     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00284-004-4305-8     Document Type: Article

References (18)

1. Aoyama M, Toma C, Yasud M, Iwanaga M (2000) Sequence of the gene encoding an alkaline serine proteinase of Bacillus pumilus TYO-67. Microbiol Immunol 44:389-393
2. Debabov VG (1982) The industrial use of bacilli. In: The molecular biology of bacilli. New York: Academic Press
3. Dubnau D, Davidoff-Abelson R (1971) Fate of transforming DNA following uptake by competent Bacillus subtilis. 1. Formation and properties of the donor-recipient complex. J Mol Biol 56:209-221
4. Hiroaki I, Nojima H, Okayama H (1996) High efficiency transformation of Escherichia coli with plasmids. Gene 96:23-28
5. Jacobs M, Eliasson M, Uhlen M, Flock JI (1985) Cloning, sequencing and expression of subtilisin Carlsberg from Bacillus licheniformis. Nucleic Acids Res 13:8913-8926
6. Jang JS, Kang DO, Chun MJ, Byun SM (1992) Molecular cloning of a subtilisin J gene from Bacillus stearothermophilus and its expression in Bacillus subtilis. Biochem Biophys Res Commun 184:277-282
7. Jonczyk W, Studniarski K (1986) Enzyme dehairing of pigskins. J Soc Leather Technol Chemists 72:83-88
8. Jorgensen PL, Tangney M, Pedersen PE, Hastrup S, Diderichsen B, Jorgensen ST (2000) Cloning and sequencing of an alkaline protease gene from Bacillus lentus and amplification of the gene on the B. lentus chromosome by an improved technique. Appl Environ Microbiol 66:825-827
9. Koide Y, Nakamura A, Uozumi T, Beppu T (1986) Cloning and sequencing of the major intracellular serine protease gene of Bacillus subtilis. J Bacteriol 167:110-116
10. Nakamura T, Yamagata Y, Ichishima E (1992) Nucleotide sequence of the subtilisin NAT gene, aprN, of Bacillus subtilis (natto). Biosci Biotechnol Biochem 56:1869-1871
11. Jiao Pan, Yizheng Zhang, et al (2004) Cloning and characterization of gene promoters from Bacillus pumilus. High Technol Lett China, in press
12. Puvanakrishnan R, Dhar SC (1986) Recent advances in the enzymatic depilation of hides and skins. Leather Sci 33:177-191
13. Qing Huang, Yong Peng, Yizheng Zhang, et al (2003) Purification and characterization of an alkaline serine protease with dehairing function from Bacillus pumilus. Curr Microbiol 46:169-173
14. Sambrook J, Fristsh EF, Maniatis T (eds) (1989) Molecular cloning: A laboratory manual, 2nd edn. New York: Cold Spring Harbor Laboratory
15. Wong SL, Ye R, Nathoo S (1994) Engineering and production of streptokinase in a Bacillus subtilis expression-secretion system. Appl Environ Microbiol 60:517-523
16. Xu-Chu Wu, et al (1991) Engineering a Bacillus subtilis expression-secretion system with a strain deficient in six extracellular protease. J Bacteriol 173:4952-4958
17. Yang S, Huang H, Zhang R, et al (2001) Expression and purification of extracellular penicillin G acylase in Bacillus subtilis. Protein Expr Purif 21:60-64
18. Yizheng Zhang (2000) Patent: 1361278A China