In vivo site-directed mutagenesis using oligonucleotides

Nature Biotechnology

Volumn 19, Issue 8, 2001, Pages 773-776

  Storici, F ^a   Lewis, L K ^a   Resnick, M A ^a  

^a NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

OLIGONUCLEOTIDE;

ARTICLE; DNA SEQUENCE; GENE CASSETTE; GENE DELETION; GENE MUTATION; IN VIVO STUDY; MOLECULAR CLONING; PRIORITY JOURNAL; PROTEIN MODIFICATION; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS;

BASE SEQUENCE; ESCHERICHIA COLI; GENETIC VECTORS; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; MUTAGENESIS, SITE-DIRECTED; MUTATION; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; TRANSFORMATION, GENETIC;

EID: 0034903337     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/90837     Document Type: Article

References (22)

1. Yeast as an honorary mammal
2. Replacement of chromosome segments with altered DNA sequences constructed in vitro
3. Site-directed, recombination-mediated mutagenesis of a complex gene locus
4. A method for performing precise alterations in the yeast genome using a recycable selectable marker
5. Cloning-free PCR-based allele replacement methods
6. Transformation of yeast with synthetic oligonucleotides
7. Targeted deletions created in yeast vectors by recombinational excision
8. Direct isolation of human BRCA2 gene by transformation-associated recombination in yeast
9. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae
10. Tying up loose ends: Nonhomologous end-joining in Saccharomyces cerevisiae
11. Recombination factors of Saccharomyces cerevisiae
12. Parameters affecting the frequencies of transformation and co-transformation with synthetic oligonucleotides in yeast
13. Production of transgenic mice with yeast artificial chromosomes
14. A simple and efficient method for making site-directed mutants, deletions, and fusions of large DNA such as P1 and BAC clones
15. Directed mutagenesis of YAC-cloned DNA using a rapid, PCR-based screening protocol
16. Point mutation of bacterial artificial chromosomes by ET recombination
17. Efficient gene targeting in the moss Physcomitrella patens
18. Efficient modification of human chromosomal alleles using recombination-proficient chicken/human microcell hybrids
19. Designer deletion strains derived from Saccharomyces cerevisiae S288C: A useful set of strains and plasmids for PCR-mediated gene disruption and other applications
20. Requirement for end-joining and checkpoint functions, but not RAD52-mediated recombination, after EcoRI endonuclease cleavage of Saccharomyces cerevisiae DNA
21. Disruption of seven hypothetical aryl alcohol dehydrogenase genes from Saccharomyces cerevisiae and construction of a multiple knock-out strain
22. A mutational analysis of the yeast proliferating cell nuclear antigen indicates distinct roles in DNA replication and DNA repair