Two-step method for constructing unmarked insertions, deletions and allele substitutions in the yeast genome

FEMS Microbiology Letters

Volumn 248, Issue 1, 2005, Pages 31-36

  Gray, Misa ^a   Piccirillo, Sarah ^a   Honigberg, Saul M ^a  

^a UNIVERSITY OF MISSOURI KANSAS CITY   (United States)

Author keywords

Deletion; Insertion; PCR; Saccharomyces cerevisiae; Site specific genomic mutagenesis; Tandem affinity purification

Indexed keywords

OLIGONUCLEOTIDE; PROTEIN; RECOMBINANT RECEPTOR; TANDEM AFFINITY PROTEIN; UNCLASSIFIED DRUG;

ACCURACY; ALLELISM; ARTICLE; DNA SYNTHESIS; GENE CONSTRUCT; GENE DELETION; GENE INSERTION; GENE INSERTION SEQUENCE; GENE SEQUENCE; GENOME; MOLECULAR CLONING; NONHUMAN; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PRODUCTIVITY; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS;

ALLELES; GENE DELETION; GENES, FUNGAL; GENETIC ENGINEERING; GENOME, FUNGAL; MUTAGENESIS; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE; SACCHAROMYCETALES;

EID: 20444507868     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.femsle.2005.05.018     Document Type: Article

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