Identification of Claisen cyclase domain in fungal polyketide synthase WA, a naphthopyrone synthase of Aspergillus nidulans

Chemistry and Biology

Volumn 8, Issue 2, 2001, Pages 189-197

  Fujii, Isao ^a   Watanabe, Akira ^a   Sankawa, Ushio ^a   Ebizuka, Yutaka ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

Claisen cyclase; Deletion mutant; Naphthopyrone; Polyketide synthase; Site directed mutagenesis; Thioesterase

Indexed keywords

MULTIENZYME COMPLEX;

AMINO ACID SEQUENCE; ARTICLE; ASPERGILLUS NIDULANS; CHEMISTRY; ENZYMOLOGY; GENE DELETION; GENETICS; MOLECULAR GENETICS; PROTEIN FOLDING; PROTEIN MOTIF; SITE DIRECTED MUTAGENESIS;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ASPERGILLUS NIDULANS; MOLECULAR SEQUENCE DATA; MULTIENZYME COMPLEXES; MUTAGENESIS, SITE-DIRECTED; PROTEIN FOLDING; SEQUENCE DELETION;

ASPERGILLUS; BACTERIA (MICROORGANISMS); EMERICELLA NIDULANS; ILEX COOKII;

EID: 0035068075     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1074-5521(00)90068-1     Document Type: Article

References (40)

1. Bioactive microbial products: Focus upon mechanism of action
2. Polyketide synthase gene manipulation: A structure-function approach in engineering novel antibiotics
3. Genetic contributions to understanding polyketide synthase
4. Combinatorial biosynthesis for new drug discovery
5. Engineering of modular polyketide synthases to produce novel polyketides
6. Multiple genetic modifications of the erythromycin gene cluster to produce a library of novel 'unnatural' natural products
7. Modular organization of genes required for complex polyketide biosynthesis
8. Nucleotide sequence and deduced functions of a set of co-transcribed genes of Streptomyces coelicolor A3 including the polyketide synthase for the antibiotic actinorhodin
9. Nucleotide sequence, transcription and deduced function of a gene involved in polyketide antibiotic synthesis in Streptomyces coelicolor
10. The multifunctional 6-methylsalicylic acid synthase gene of Penicillium patulum. Its gene structure relative to that of other polyketide synthases
11. Cloning of the polyketide synthase gene atX from Aspergillus terreus and its identification as the 6-methylsalicylic acid synthase gene by heterologous expression
12. Heterologous expression and product identification of Colletotrichum lagenarium polyketide synthase encoded by the PKS1 gene involved in melanin biosynthesis
13. A polyketide synthase is required for fungal virulence and production of the polyketide T-toxin
14. Lovastatin biosynthesis in Aspergillus terreus: Characterization of blocked mutants, enzyme activities and multifunctional polyketide synthase gene
15. Modulation of polyketide synthase activity by accessory proteins during lovastatin biosynthesis
16. Polyketide biosynthesis in filamentous fungi
17. The animal fatty acid synthase: One gene, one polypeptide, seven enzymes
18. Product identification of polyketide synthase encoded by Aspergillus nidulans wA gene
19. Re-identification of Aspergillus nidulans wA gene to code for a polyketide synthase of naphthopyrone
20. Enzymatic synthesis of 1,3,6,8-tetrahydroxynaphthalene solely from malonyl coenzyme A by a fungal iterative Type I polyketide synthase PKS1
21. Aspergillus fumigatus alb1 encodes naphthopyrone synthase when expressed in Aspergillus oryzae
22. Isolation and molecular characterization of the Aspergillus nidulans wA gene
23. The developmentally regulated Aspergillus nidulans wA gene encodes a polypeptide homologous to polyketide and fatty acid synthases
24. The thioesterase of the erythromycin-producing polyketide synthase: Mechanistic studies in vitro to investigate its mode of action and substrate specificity
25. Biosynthesis of 6-methylsalicylic acid
26. Structures and functional analyses of fungal polyketide synthase genes
27. 2H-labelled acetates
28. Demonstration of an alternative chain folding in the pyranonaphthalene fonsecin
29. On the question of half- or full-site reactivity of animal fatty acid synthetase
30. Manipulation of macrolide ring size by directed mutagenesis of a modular polyketide synthase
31. Structural analysis of PKS1, a polyketide synthase gene involved in melanin biosynthesis of Colletotrichum lagenarium
32. Sterigmatocystin biosynthesis in Aspergillus nidulans requires a novel type I polyketide synthase
33. Characterization of the polyketide synthase gene (pksL1) required for aflatoxin biosynthesis in Aspergillus parasiticus
34. Structure of a myristoyl-ACP-specific thioesterase from Vibrio harveyi
35. The crystal structure of palmitoyl protein thioesterase 1 and the molecular basis of infantile neuronal ceroid lipofuscinosis
36. The three-dimensional structure of 4-hydroxybenzoyl-CoA thioesterase from Pseudomonas sp. strain CBS-3
37. Crystal structure of the Escherichia coli thioesterase II, a homolog of the human Nef binding enzyme
38. Role of a buried acid group in the mechanism of action of chymotrypsin
39. Integrative transformation of Aspergillus oryzae with a plasmid containing the Aspergillus nidulans argB gene