A conserved hydrogen-bond network in the catalytic centre of animal glutaminyl cyclases is critical for catalysis

Biochemical Journal

Volumn 411, Issue 1, 2008, Pages 181-190

  Huang, Kai Fa ^a,b   Wang, Yu Ruei ^a   Chang, En Cheng ^a   Chou, Tsung Lin ^c   Wang, Andrew H J ^a,b,c  

^a INSTITUTE OF BIOLOGICAL CHEMISTRY   (Taiwan)

^b ACADEMIA SINICA   (Taiwan)

^c NATIONAL TAIWAN UNIVERSITY   (Taiwan)

Author keywords

Glutaminyl cyclase (glutaminyl peptide cyclotransferase, QC); Hydrogen bond network; Proton transfer; Pyroglutamate (pGlu); Site directed mutagenesis; Synchrotron; X ray crystallography

Indexed keywords

GLUTAMINYL CYCLASE (GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE, QC); HYDROGEN-BOND NETWORKS; PYROGLUTAMATE (PGLU); SITE-DIRECTED MUTAGENESIS;

CATALYSIS; HYDROGEN BONDS; MUTAGENESIS; PATHOLOGY; PEPTIDES; PROTON TRANSFER; SYNCHROTRON RADIATION; X RAY CRYSTALLOGRAPHY;

ENZYMES;

ASPARTIC ACID; CARBOXYL GROUP; ENZYME; GLUTAMIC ACID; GLUTAMINYL CYCLASE; HISTONE; PEPTIDASE; SERINE; UNCLASSIFIED DRUG; WATER;

ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME KINETICS; ENZYME STRUCTURE; HUMAN; HYDROGEN BOND; HYDROPHILICITY; MUTATION; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN STABILITY; PROTON TRANSPORT; STEADY STATE;

AMINOACYLTRANSFERASES; ANIMALS; CATALYSIS; CATALYTIC DOMAIN; CLONING, MOLECULAR; CRYSTALLOGRAPHY, X-RAY; HUMANS; HYDROGEN BONDING; KINETICS; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION;

ANIMALIA;

EID: 41649084118     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20071073     Document Type: Article

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