Pyroglutamyl-peptidase I: Cloning, sequencing, and characterisation of the recombinant human enzyme

Protein Expression and Purification

Volumn 28, Issue 1, 2003, Pages 111-119

  Dando, Pam M ^a   Fortunato, Mara ^a   Strand, Gry B ^a,b   Smith, Trevor S ^a   Barrett, Alan J ^a  

^a BABRAHAM INSTITUTE   (United Kingdom)

^b UNIVERSITY OF OSLO   (Norway)

Author keywords

Cysteine peptidase; EC 3.4.19.3; Peptidase family C15; PGPEP1; Pyroglutamyl peptidase I; Sf9 insect cell

Indexed keywords

ARCHAEA; BACILLUS AMYLOLIQUEFACIENS; BACTERIA (MICROORGANISMS); INSECTA; MAMMALIA; PROKARYOTA; SPODOPTERA; SPODOPTERA FRUGIPERDA; THERMOCOCCUS; THERMOCOCCUS LITORALIS; UNIDENTIFIED BACULOVIRUS; VERTEBRATA;

EID: 0037361122     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1046-5928(02)00632-0     Document Type: Article

References (29)

1. A. Szewczuk, J. Kwiatkowska, Pyrrolidonyl peptidase in animal, plant and human tissues. Occurrence and some properties of the enzyme, Eur. J. Biochem. 15 (1970) 92-96.
2. J. Robert-Baudouy, P. Clauziat, G. Thierry, in: A.J. Barrett, N.D. Rawlings, J.F. Woessner (Eds.), Handbook of Proteolytic Enzymes, Academic Press, London, 1998, pp. 791-795.
3. A.C. Awadé, P. Cleuziat, T. Gonzalès, J. Robert-Baudouy, Pyrrolidone carboxyl peptidase (Pcp): an enzyme that removes pyroglutamic acid (pGlu) from pGlu-peptides and pGlu-proteins, Proteins: Struct. Funct. Genet. 20 (1994) 34-51.
4. T. Yoshimoto, T. Shimoda, A. Kitazono, T. Kabashima, K. Ito, D. Tsuru, Pyroglutamyl peptidase gene from Bacillus amyloliquefaciens. Cloning, sequencing, expression, and crystallization of the expressed enzyme, J. Biochem. (Tokyo) 113 (1993) 67-73.
5. Y. Odagaki, A. Hayashi, K. Okada, K. Hirotsu, T. Kabashima, K. Ito, T. Yoshimoto, D. Tsuru, M. Sato, J. Clardy, The crystal structure of pyroglutamyl peptidase I from Bacillus amyloliquefaciens reveals a new structure for a cysteine protease, Struct. Fold. Des. 7 (1999) 399-411.
6. M.R. Singleton, M.N. Isupov, J.A. Littlechild, X-ray structure of pyrrolidone carboxyl peptidase from the hyperthermophilic archaeon Thermococcus litoralis, Struct. Fold. Des. 7 (1999) 237-244.
7. A.J. Barrett, N.D. Rawlings, Evolutionary lines of cysteine peptidases, Biol. Chem. 382 (2001) 727-733.
8. H. Tanaka, M. Chinami, T. Mizushima, K. Ogasahara, M. Ota, T. Tsukihara, K. Yutani, X-ray crystalline structures of pyrrolidone carboxyl peptidase from a hyperthermophile, Pyrococcus furiosus, and its Cys-free mutant, J. Biochem. (Tokyo) 130 (2001) 107-118.
9. A. Anastasi, M.A. Brown, A.A. Kembhavi, M.J.H. Nicklin, C.A. Sayers, D.C. Sunter, A.J. Barrett, Cystatin, a protein inhibitor of cysteine proteinases. Improved purification from egg white, characterization, and detection in chicken serum, Biochem. J. 211 (1983) 129-138.
10. A.J. Barrett, N.D. Rawlings, E.A. O'Brien, J. Struct. Biol. 134 (2001) 95-102.
11. V.A. Luckow, S.C. Lee, G. F. Barry, P.O. Olins, Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli, J. Virol. 67 (1993) 4566-4579.
12. A.A. Kembhavi, D.J. Buttle, C.G. Knight, A.J. Barrett, The two cysteine endopeptidases of legume seeds: purification and characterization by use of specific fluorometric assays, Arch. Biochem. Biophys. 303 (1993) 208-213.
13. N.D. Rawlings, A.J. Barrett, FLUSYS: a software package for the collection and analysis of kinetic and scanning data from Perkin-Elmer fluorimeters, Comput. Appl. Biosci. 6 (1990) 118-119.
14. H.T. Johansen, C.G. Knight, A.J. Barrett, Colorimetric and fluorimetric microplate assays for legumain and a staining reaction for detection of the enzyme after electrophoresis, Anal. Biochem. 273 (1999) 278-283.
15. M.M. Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72 (1976) 248-254.
16. J.F. Morrison, Kinetics of the reversible inhibition of enzyme catalysed reactions by tight-binding inhibitors, Biochim. Biophys. Acta 185 (1969) 269-286.
17. P.M. Dando, M. Fortunato, L. Smith, C.G. Knight, J.E. McKendrick, A.J. Barrett, Pig kidney legumain: an asparaginyl endopeptidase with restricted specificity, Biochem. J. 339 (1999) 743-749.
18. P.M. Cummins, B. O'Connor, Pyroglutamyl peptidase: an overview of the three known enzymatic forms, Biochim. Biophys. Acta 1429 (1998) 1-17.
19. D. Tsuru, K. Sakabe, T. Yoshimoto, K. Fujiwara, Pyroglutamyl peptidase from chicken liver: purification and some properties, J. Pharmacobiodyn. 5 (1982) 859-868.
20. D. Tsuru, K. Fujiwara, K. Kado, Purification and characterization of L-pyrrolidonecarboxylate peptidase from Bacillus amyloiliquefaciens, J. Biochem. (Tokyo) 84 (1978) 467-476.
21. U.G. McKeon, B. O'Connor, in: A.J. Barrett, N.D. Rawlings, J.F. Woessner (Eds.), Handbook of Proteolytic Enzymes, Academic Press, London, 1998, pp. 796-797.
22. P.M. Cummins, B. O'Connor, Bovine brain pyroglutamyl aminopeptidase (type-1). Purification and characterisation of a neuropeptide-inactivating peptidase, Int. J. Biochem. Cell Biol. 28 (1996) 883-893.
23. A.W. Mudge, R.E. Fellows, Bovine pituitary pyrrolidonecarboxylyl peptidase, Endocrinology 93 (1973) 1428-1434.
24. R.F. Doolittle, R.W. Armentrout, Pyrrolidonyl peptidase. An enzyme for selective removal of pyrrolidonecarboxylic acid residues from polypeptides, Biochemistry 7 (1968) 516-521.
25. D. Mantle, B. Lauffart, A. Gibson, Purification and characterization of leucyl aminopeptidase and pyroglutamyl aminopeptidase from human skeletal muscle, Clin. Chim. Acta 197 (1991) 35-45.
26. R.W. Armentrout, R.F. Doolittle, Pyrrolidonecarboxylyl peptidase: stabilization and purification, Arch. Biochem. Biophys. 132 (1969) 80-90.
27. G.S. Salvesen, H. Nagase, in: J.S. Bond, R.J. Beynon (Eds.), Proteolytic Enzymes: A Practical Approach, Oxford University Press, Oxford, 2001, pp. 105-130.
28. D.G. Higgins, J.D. Thompson, T.J. Gibson, Using CLUSTAL for multiple sequence alignments, Methods Enzymol. 266 (1996) 383-402.
29. S.F. Altschul, W. Gish, W. Miller, E.W. Myers, D.J. Lipman, Basic local alignment search tool, J. Mol. Biol. 215 (1990) 403-410.