Hexadecylphosphocholine interferes with the intracellular transport of cholesterol in HepG2 cells

FEBS Journal

Volumn 275, Issue 8, 2008, Pages 1675-1686

  Carrasco, María P ^a   Jiménez López, José M ^a   Segovia, Josefa L ^a   Marco, Carmen ^a  

^a UNIVERSITY OF GRANADA   (Spain)

Author keywords

Cholesterol homeostasis; HepG2 cells; Hexadecylphosphocholine; HMG CoA reductase; LDLr

Indexed keywords

ACETIC ACID; CHOLESTEROL; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE; LOW DENSITY LIPOPROTEIN RECEPTOR; MESSENGER RNA; MEVALONIC ACID; MILTEFOSINE; PHOSPHATIDYLCHOLINE;

ARTICLE; CELL MEMBRANE; CELL STRAIN HEPG2; CHOLESTEROL ESTERIFICATION; CHOLESTEROL SYNTHESIS; CHOLESTEROL TRANSPORT; CONTROLLED STUDY; DRUG EFFECT; ENDOPLASMIC RETICULUM; GENE EXPRESSION; HUMAN; HUMAN CELL; ISOTOPE LABELING; MEMBRANE TRANSPORT; PRIORITY JOURNAL; PROTEIN DETERMINATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

ACETATES; BIOLOGICAL TRANSPORT; CELL LINE, TUMOR; CELL MEMBRANE; CHOLESTEROL; ENDOPLASMIC RETICULUM; ENZYME ACTIVATION; ESTERS; GENE EXPRESSION REGULATION; HUMANS; LINOLEIC ACID; MEVALONIC ACID; OXO-ACID-LYASES; PHOSPHORYLCHOLINE; TRIGLYCERIDES;

EID: 41549110288     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2008.06322.x     Document Type: Article

References (37)

1. Wieder T, Orfanos CE Geilen CC (1998) Induction of ceramide-mediated apoptosis by the anticancer phospholipid analog, hexadecylphosphocholine. J Biol Chem 273, 11025 11031.
2. Engelmann J, Henke J, Willker W, Kutscher B, Nössner G, Engel J Leibfritz D (1996) Early stage monitoring of miltefosine induced apoptosis in KB cells by multinuclear NMR spectroscopy. Anticancer Res 16, 1429 1439.
3. Rakotomanga M, Blanc S, Gaudin K, Chaminade P Loiseau PM (2007) Miltefosine affects lipid metabolism in Leishmania donovani promastigotes. Antimicrob Agents Chemother 51, 1425 1430.
4. Soto J Soto P (2006) Oral miltefosine to treat leishmaniasis. Biomedica 26, 207 217.
5. Berkovic D, Wernicke JH Fleer EA (2003) Effects of etherlipid analogs on cell membrane functions. J Exp Ther Oncol 3, 185 193.
6. Jiménez-López JM, Carrasco MP, Segovia JL Marco C (2002) Hexadecylphosphocholine inhibits phosphatidylcholine biosynthesis and the proliferation of HepG2 cells. Eur J Biochem 269, 4649 4655.
7. Jiménez-López JM, Carrasco MP, Segovia JL Marco C (2004) Hexadecylphosphocholine inhibits phosphatidylcholine synthesis via both the methylation of phosphatidylethanolamine and CDP-choline pathways in HepG2 cells. Int J Biochem Cell Biol 36, 153 161.
8. Jiménez-López JM, Carrasco MP, Marco C Segovia JL (2006) Hexadecylphosphocholine disrupts cholesterol homeostasis and induces the accumulation of free cholesterol in HepG2 tumour cells. Biochem Pharmacol 71, 1114 1121.
9. Babalola JO, Wendeler M, Breiden B, Arenz C, Schwarzmann G, Locatelli-Hoops S Sandhoff K (2007) Development of an assay for the intermembrane transfer of cholesterol by Niemann-Pick C2 protein. Biol Chem 388, 617 626.
10. Soccio RE Breslow JL (2004) Intracellular cholesterol transport. Arterioscler Thromb Vasc Biol 24, 1150 1160.
11. Robinet P, Fradagrada A, Monier M, Marchetti M, Cogny A, Moatti N, Paul J, Vedie B Lamaze C (2006) Dynamin is involved in endolysosomal cholesterol delivery to the endoplasmic reticulum: role in cholesterol homeostasis. Transport 7, 811 823.
12. Lange Y, Ye J Chin J (1997) The fate of cholesterol exiting lysosomes. J Biol Chem 272, 17018 17022.
13. Liscum L Munn NJ (1999) Intracellular cholesterol transport. Biochim Biophys Acta 1438, 19 37.
14. Prinz W (2002) Cholesterol trafficking in the secretory and endocytic systems. Semin Cell Dev Biol 13, 197 203.
15. Field FJ, Born E, Murthy S Mathur SN (1998) Transport of cholesterol from the endoplasmic reticulum to the plasma membrane is constitutive in CaCo-2 cells and differs from the transport of plasma membrane cholesterol to the endoplasmic reticulum. J Lipid Res 39, 333 343.
16. Lange Y, Ye J Steck TL (1998) Circulation of cholesterol between lysosomes and the plasma membrane. J Biol Chem 273, 18915 18922.
17. Mohammadi A, Perry RJ, Storey MK, Cook HW, Byers DM Ridgway ND (2001) Golgi localization and phosphorylation of oxysterol binding protein in Niemann-Pick C and U18666A-treated cells. J Lipid Res 42, 1062 1071.
18. Blanchette-Mackie EJ (2000) Intracellular cholesterol trafficking: role of the NPC1 protein. Biochim Biophys Acta 1486, 171 183.
19. Lange Y, Ye J, Rigney M Steck TL (2002) Dynamics of lysosomal cholesterol in Niemann-Pick type C and normal human fibroblasts. J Lipid Res 43, 198 204.
20. Wojtanik KM Liscum L (2003) The transport of low density lipoprotein-derived cholesterol to the plasma membrane is defective in NPC1 cells. J Biol Chem 278, 14850 14856.
21. Lange Y (1994) Cholesterol movement from plasma membrane to rough endoplasmic reticulum. Inhibition by progesterone. J Biol Chem 269, 3411 3414.
22. Chang T, Chang CCY, Ohgami N Yamauchi Y (2006) Cholesterol sensing, trafficking, and esterification. Annu Rev Cell Dev Biol 22, 129 157.
23. Goldstein JL, DeBose-Boyd RA Brown MS (2006) Protein sensors for membrane sterols. Cell 124, 35 46.
24. Sugii S, Reid PC, Ohgami N, Du H Chang T (2003) Distinct endosomal compartments in early trafficking of low density lipoprotein-derived cholesterol. J Biol Chem 278, 27180 27189.
25. Lange Y, Strebel F Steck TL (1993) Role of the plasma membrane in cholesterol esterification in rat hepatoma cells. J Biol Chem 268, 13838 13843.
26. Cheng D, Chang CC, Qu X Chang TY (1995) Activation of acyl-coenzyme A:cholesterol acyltransferase by cholesterol or by oxysterol in a cell-free system. J Biol Chem 270, 685 695.
27. Schneiter R (2007) Intracellular sterol transport in eukaryotes, a connection to mitochondrial function? Biochimie 89, 255 259.
28. Lange Y Steck TL (1997) Quantitation of the pool of cholesterol associated with acyl-CoA:cholesterol acyltransferase in human fibroblasts. J Biol Chem 272, 13103 13108.
29. Härmälä AS, Pörn MI, Mattjus P Slotte JP (1994) Cholesterol transport from plasma membranes to intracellular membranes is inhibited by 3-beta-[2-(diethylamino)ethoxy]androst-5-en-17-one. Biochim Biophys Acta 1211, 317 325.
30. Maxfield FR Wüstner D (2002) Intracellular cholesterol transport. J Clin Invest 110, 891 898.
31. Yamauchi Y, Reid PC, Sperry JB, Furukawa K, Takeya M, Chang CC Chang TY (2007) Plasma membrane rafts complete cholesterol synthesis by participating in retrograde movement of precursor sterols. J Biol Chem 282, 34994 35004.
32. Bligh EG Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37, 911 917.
33. Sugii S, Lin S, Ohgami N, Ohashi M, Chang CCY Chang T (2006) Roles of endogenously synthesized sterols in the endocytic pathway. J Biol Chem 281, 23191 23206.
34. Goldstein JL, Dana SE, Faust JR, Beaudet AL Brown MS (1975) Role of lysosomal acid lipase in the metabolism of plasma low density lipoprotein. Observations in cultured fibroblasts from a patient with cholesteryl ester storage disease. J Biol Chem 250, 8487 8495.
35. Marco C, Alejandre MJ, González-Pacanowska D, Segovia JL García-Peregrín E (1985) Evolution of 3-hydroxy-3-methylglutaryl- CoA reductase and microsomal membrane fluidity throughout chick embryo development. Int J Biochem 17, 271 274.
36. Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29, 2002 2007.
37. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248 254.