Direct spectroscopic study of reconstituted transcription complexes reveals that intrinsic termination is driven primarily by thermodynamic destabilization of the nucleic acid framework

Journal of Biological Chemistry

Volumn 283, Issue 6, 2008, Pages 3537-3549

  Datta, Kausiki ^a   Von Hippel, Peter H ^a  

^a UNIVERSITY OF OREGON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING SITES; BIOMOLECULES; DICHROISM; DNA; ELECTRIC FIELD EFFECTS; FUNCTIONAL POLYMERS; GENES; OPTICAL PROPERTIES; ORGANIC ACIDS; POLYMERS; RNA; SCAFFOLDS; SPECTROSCOPIC ANALYSIS; TRANSCRIPTION;

ACTIVE SITES; AMINOPURINE; BASE PAIRS; COMPLEX DISSOCIATIONS; DNA TRANSCRIPTIONS; ELONGATION COMPLEXES; FLUORESCENCE SPECTRUMS; NUCLEIC ACID SCAFFOLDS; RNA POLYMERASES; SPECTROSCOPIC STUDIES; TEMPLATE BASE; TRANSCRIPTION COMPLEXES; UV CIRCULAR DICHROISMS;

NUCLEIC ACIDS;

2 AMINOPURINE; CYSTEINE; GLYCINE; RNA POLYMERASE; SINGLE STRANDED RNA; T7 RNA POLYMERASE; UNCLASSIFIED DRUG; BACTERIAL DNA; BACTERIAL RNA; BACTERIOPHAGE T7 RNA POLYMERASE; DNA DIRECTED RNA POLYMERASE; NUCLEIC ACID; VIRUS PROTEIN;

ARTICLE; BASE PAIRING; CIRCULAR DICHROISM; DNA RNA HYBRIDIZATION; DNA TRANSCRIPTION; ENZYME ACTIVE SITE; FLUORESCENCE SPECTROSCOPY; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RNA BINDING; RNA STRUCTURE; THERMODYNAMICS; BINDING SITE; BIOLOGICAL MODEL; CHEMISTRY; CONFORMATION; ESCHERICHIA COLI; GENETIC TRANSCRIPTION; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; REGULATOR GENE; SPECTROPHOTOMETRY;

BASE SEQUENCE; BINDING SITES; CIRCULAR DICHROISM; DNA, BACTERIAL; DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; NUCLEIC ACIDS; RNA, BACTERIAL; SPECTROPHOTOMETRY; TERMINATOR REGIONS (GENETICS); THERMODYNAMICS; TRANSCRIPTION, GENETIC; VIRAL PROTEINS;

EID: 41249091442     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M707998200     Document Type: Article

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