The PtdIns(4,5)P2 Ligand Itself Influences the Localization of PKCα in the Plasma Membrane of Intact Living Cells

Journal of Molecular Biology

Volumn 377, Issue 4, 2008, Pages 1038-1052

  Marín Vicente, Consuelo ^a   Nicolás, Francisco E ^a   Gómez Fernández, Juan C ^a   Corbalán García, Senena ^a  

^a UNIVERSITY OF MURCIA   (Spain)

Author keywords

C2 domain; PKC; PtdIns(4,5)P2; rapamycin triggered heterodimerization; siRNA

Indexed keywords

ADENOSINE TRIPHOSPHATE; PHOSPHATE; PHOSPHATIDYLINOSITOL(4,5) BISPHOSPHATE; PROTEIN KINASE C ALPHA; RAP PROTEIN; RNA; SMALL INTERFERING RNA; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CELL MEMBRANE; CELL STRAIN; CONTROLLED STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; PROTEIN LOCALIZATION;

EID: 40849111154     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2007.12.011     Document Type: Article

References (51)

1. McLaughlin S., and Murray D. Plasma membrane phosphoinositide organization by protein electrostatics. Nature 438 (2005) 605-611
2. Di Paolo G., and De Camilli P. Phosphoinositides in cell regulation and membrane dynamics. Nature 443 (2006) 651-657
3. Ohno S., and Nishizuka Y. Protein kinase C isotypes and their specific functions: prologue. J. Biochem. (Tokyo) 132 (2002) 509-511
4. Corbalan-Garcia S., and Gomez-Fernandez J.C. Protein kinase C regulatory domains: the art of decoding many different signals in membranes. Biochim. Biophys. Acta 1761 (2006) 633-654
5. Cho W., and Stahelin R.V. Membrane binding and subcellular targeting of C2 domains. Biochim. Biophys. Acta 1761 (2006) 838-849
6. 2+. Structure 6 (1998) 1395-1405
7. Verdaguer N., Corbalan-Garcia S., Ochoa W., Fita I., and Gomez-Fernandez J. Ca(2 +) bridges the C2 membrane-binding domain of protein kinase Calpha directly to phosphatidylserine. EMBO J. 18 (1999) 6329-6338
8. Conesa-Zamora P., Gomez-Fernandez J., and Corbalan-Garcia S. The C2 domain of protein kinase calpha is directly involved in the diacylglycerol-dependent binding of the C1 domain to the membrane. Biochim. Biophys. Acta 1487 (2000) 246-254
9. Ochoa W., Corbalan-Garcia S., Eritja R., Rodriguez-Alfaro J., Gomez-Fernandez J., Fita I., and Verdaguer N. Additional binding sites for anionic phospholipids and calcium ions in the crystal structures of complexes of the C2 domain of protein kinase calpha. J. Mol. Biol. 320 (2002) 277-291
10. Corbalan-Garcia S., Garcia-Garcia J., Rodriguez-Alfaro J., and Gomez-Fernandez J. A new phosphatidylinositol 4,5-bisphosphate-binding site located in the C2 domain of protein kinase Calpha. J. Biol. Chem. 278 (2003) 4972-4980
11. 2+ and PIP2 recognition by its C2 domain. Mol. Biol. Cell 17 (2006 (January)) 56-66
12. 2 sensing domain: a new insight into an old pathway. J. Mol. Biol. 362 (2006) 901-914
13. 2+ affinity. Biochemistry 46 (2007) 4322-4336
14. 2-sensing domains with different affinities for membrane binding. J. Mol. Biol. 371 (2007) 608-621
15. Nishizuka Y. Protein kinase C and lipid signaling for sustained cellular responses. FASEB J. 9 7 (1995, April) 484-496
16. 2+ influx and phosphatidylinositol 4,5-bisphosphate in differentiated PC12 cells. Mol. Biol. Cell 16 (2005) 2848-2861
17. Banaszynski L., and Wandless T. Conditional control of protein function. Chem. Biol. 13 (2006) 11-21
18. Ishihara H., Shibasaki Y., Kizuki N., Wada T., Yazaki Y., Asano T., and Oka Y. Type I phosphatidylinositol-4-phosphate 5-kinases. Cloning of the third isoform and deletion/substitution analysis of members of this novel lipid kinase family. J. Biol. Chem. 273 (1998) 3625-3634
19. Raucher D., Stauffer T., Chen W., Shen K., Guo S., York J.D., et al. Phosphatidylinositol 4,5-bisphosphate functions as a second messenger that regulates cytoskeleton-plasma membrane adhesion. Cell 100 (2000) 221-228
20. Inoue T., Heo W.D., Grimley J.S., Wandless T.J., and Meyer T. An inducible translocation strategy to rapidly activate and inhibit small GTPase signaling pathways. Nat. Methods 2 (2005) 415-418
21. 2 lipids target proteins with polybasic clusters to the plasma membrane. Science 314 (2006) 1458-1461
22. 2 gate KCNQ ion channels. Science 314 (2006) 1454-1457
23. Varnai P., Thyagarajan B., Rohacs T., and Balla T. Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate levels influence multiple regulatory functions of the lipid in intact living cells. J. Cell Biol. 175 (2006) 377-382
24. van Zeijl L., Ponsioen B., Giepmans B.N.G., Ariaens A., Postma F.R., Varnai P., et al. Regulation of connexin43 gap junctional communication by phosphatidylinositol 4,5-bisphosphate. J. Cell Biol. 177 (2007) 881-891
25. Kwik J., Boyle S., Fooksman D., Margolis L., Sheetz M.P., and Edidin M. Membrane cholesterol, lateral mobility, and the phosphatidylinositol 4,5-bisphosphate-dependent organization of cell actin. Proc. Natl. Acad. Sci. U. S. A. 100 (2003, November 25) 13964-13969
26. 2-dependent microdomain assemblies capture microtubules to promote and control leading edge motility. J. Cell Biol. 169 (2005) 151-165
27. Garcia P., Gupta R., Shah S., Morris A.J., Rudge S.A., Scarlata S., et al. The pleckstrin homology domain of phospholipase C-delta 1 binds with high affinity to phosphatidylinositol 4,5-bisphosphate in bilayer membranes. Biochemistry 34 (1995) 16228-16234
28. Rebecchi M.J., and Pentyala S.N. Structure, function, and control of phosphoinositide-specific phospholipase C. Physiol. Rev. 80 (2000) 1291-1335
29. 2 concentration monitored in living cells. Curr. Biol. 8 (1998) 343-346
30. Newton A.C. Protein kinase C: structural and spatial regulation by phosphorylation, cofactors, and macromolecular interactions. Chem. Rev. 101 (2001) 2353-2364
31. Parker P.J., and Murray-Rust J. PKC at a glance. J. Cell Sci. 117 (2004) 131-132
32. Jacobson K., Mouritsen O., and Anderson R. Lipid rafts: at a crossroad between cell biology and physics. Nat. Cell Biol. 9 (2007) 7-14
33. Oancea E., and Meyer T. Protein kinase C as a molecular machine for decoding calcium and diacylglycerol signals. Cell 95 (1998) 307-318
34. Lopez-Nicolas R., Lopez-Andreo M.J., Marin-Vicente C., Gomez-Fernandez J.C., and Corbalan-Garcia S. Molecular mechanisms of protein kinase C-alpha localization and activation by arachidonic acid. The C2 domain also plays a role. J. Mol. Biol. 357 (2006) 1105-1120
35. Reither G., Schaefer M., and Lipp P. PKCalpha: a versatile key for decoding the cellular calcium toolkit. J. Cell Biol. 174 (2006) 521-533
36. Guirland C., Suzuki S., Kojima M., Lu B., and Zheng J.Q. Lipid rafts mediate chemotropic guidance of nerve growth cones. Neuron 42 (2004) 51-62
37. Golub T., Wacha S., and Caroni P. Spatial and temporal control of signaling through lipid rafts. Curr. Opin. Neurobiol. 14 (2004) 542-550
38. Wen Z., and Zheng J. Directional guidance of nerve growth cones. Curr. Opin. Neurobiol. 16 (2006) 52-58
39. Choe Y., Lee B., and Kim K. Participation of protein kinase C alpha isoform and extracellular signal-regulated kinase in neurite outgrowth of GT1 hypothalamic neurons. J. Neurochem. 83 (2002) 1412-1422
40. Schmidt J., Fleming M., and Leu B. Presynaptic protein kinase C controls maturation and branch dynamics of developing retinotectal arbors: possible role in activity-driven sharpening. J. Neurobiol. 58 (2004) 328-340
41. Gatlin J., Estrada-Bernal A., Sanford S., and Pfenninger K. Myristoylated, alanine-rich C-kinase substrate phosphorylation regulates growth cone adhesion and pathfinding. Mol. Biol. Cell 17 (2006) 5115-5130
42. Das K., Freudenrich T., and Mundy W. Assessment of PC12 cell differentiation and neurite growth: a comparison of morphological and neurochemical measures. Neurotoxicol. Teratol. 26 (2004) 397-406
43. Conrad S., Genth H., Hofmann F., Just I., and Skutella T. Neogenin-RGMa signaling at the growth cone is bone morphogenetic protein-independent and involves RhoA, ROCK, and PKC. J. Biol. Chem. 282 (2007) 16423-16433
44. 2H-NMR study and molecular dynamics simulation of the location, alignment, and mobility of pyrene in POPC bilayers. Biophys. J. 88 (2005) 1818-1827
45. Nalefski E.A., and Newton A.C. Membrane binding kinetics of protein kinase C betaII mediated by the C2 domain. Biochemistry 40 (2001) 13216-13229
46. 2+/phosphatidylserine binding region of the C2 domain in the translocation of protein kinase Calpha to the plasma membrane. J. Biol. Chem. 278 (2003) 10282-10290
47. Rodriguez-Alfaro J., Gomez-Fernandez J., and Corbalan-Garcia S. Role of the lysine-rich cluster of the C2 domain in the phosphatidylserine-dependent activation of PKCalpha. J. Mol. Biol. 335 (2004) 1117-1129
48. Spencer D.M., Wandless T.J., Schreiber S.L., and Crabtree G.R. Controlling signal transduction with synthetic ligands. Science 262 (1993) 1019-1024
49. Kim D.-H., Behlke M.A., Rose S.D., Chang M.-S., Choi S., and Rossi J.J. Synthetic dsRNA Dicer substrates enhance RNAi potency and efficacy. Nat. Biotechnol. 23 (2005) 222-226
50. Conesa-Zamora P., Lopez-Andreo M., Gomez-Fernandez J., and Corbalan-Garcia S. Identification of the phosphatidylserine binding site in the C2 domain that is important for PKC alpha activation and in vivo cell localization. Biochemistry 40 (2001) 13898-13905
51. Meyer T., and Oancea E. Studies of signal transduction events using chimeras to green fluorescent protein. Methods Enzymol. 327 (2000) 500-513