Dynamic behavior of GFP-CLIP-170 reveals fast protein turnover on microtubule plus ends

Journal of Cell Biology

Volumn 180, Issue 4, 2008, Pages 729-737

  Dragestein, Katharina A ^a   Van Cappellen, Wiggert A ^a   Van Haren, Jeffrey ^a   Tsibidis, George D ^b   Akhmanova, Anna ^a   Knoch, Tobias A ^a   Grosveld, Frank ^a   Galjart, Niels ^a  

^a ERASMUS MEDICAL CENTER   (Netherlands)

^b INSTITUTE OF ELECTRONIC STRUCTURE AND LASER   (Greece)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOPLASM PROTEIN; CYTOPLASMIC LINKER PROTEIN 170; TUBULIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; MICROTUBULE; MOUSE; NONHUMAN; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN METABOLISM;

AMINO ACID MOTIFS; ANIMALS; BINDING SITES; CERCOPITHECUS AETHIOPS; COS CELLS; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; MICE; MICE, TRANSGENIC; MICROTUBULE-ASSOCIATED PROTEINS; MICROTUBULES; NEOPLASM PROTEINS; PROTEIN BINDING; PROTEIN TRANSPORT; RATS; RECOMBINANT FUSION PROTEINS; TIME FACTORS;

MAMMALIA;

EID: 40849088165     PISSN: 00219525     EISSN: 00219525     Source Type: Journal    
DOI: 10.1083/jcb.200707203     Document Type: Article

References (30)

1. Akhmanova, A., and C.C. Hoogenraad. 2005. Microtubule plus-end-tracking proteins: mechanisms and functions. Curr. Opin. Cell Biol. 17:47-54.
2. Akhmanova, A., A.-L. Mausset-Bonnefont, W. Van Cappellen, N. Keijzer, C.C. Hoogenraad, T. Stepanova, K. Drabek, J. van der Wees, M. Mommaas, J. Onderwater, et al. 2005. The microtubule plus end tracking protein CLIP-170 associates with the spermatid manchette and is essential for spermatogenesis. Genes Dev. 19:2501-2515.
3. Arnal, I., and R.H. Wade. 1995. How does taxol stabilize microtubules? Curr. Biol. 5:900-908.
4. Arnal, I., C. Heichette, G.S. Diamantopoulos, and D. Chretien. 2004. CLIP-170/tubulin-curved oligomers coassemble at microtubule ends and promote rescues. Curr. Biol. 14:2086-2095.
5. Charpilienne, A., M. Nejmeddine, M. Berois, N. Parez, E. Neumann, E. Hewat, G. Trugnan, and J. Cohen. 2001. Individual rotavirus-like particles containing 120 molecules of fluorescent protein are visible in living cells. J. Biol. Chem. 276:29361-29367.
6. Chen, Y., J.D. Muller, P.T. So, and E. Gratton. 1999. The photon counting histogram in fluorescence fluctuation spectroscopy. Biophys. J. 77:553-567.
7. Drabek, K., M. van Ham, T. Stepanova, K. Draegestein, R. van Horssen, C.L. Sayas, A. Akhmanova, T. Ten Hagen, R. Smits, R. Fodde, et al. 2006. Role of CLASP2 in microtubule stabilization and the regulation of persistent motility. Curr. Biol. 16:2259-2264.
8. Elson, E.L. 2004. Quick tour of fluorescence correlation spectroscopy from its inception. J. Biomed. Opt. 9:857-864.
9. Folker, E.S., B.M. Baker, and H.V. Goodson. 2 005. I nteractions between CLIP-170, tubulin, and microtubules: implications for the mechanism of CLIP-170 plus-end tracking behavior. Mol. Biol. Cell. 16:5373-5384.
10. Honnappa, S., O. Okhrimenko, R. Jaussi, H. Jawhari, I. Jelesarov, F.K. Winkler, and M.O. Steinmetz. 2006. Key interaction modes of dynamic +TIP networks. Mol. Cell. 23:663-671.
11. Hoogenraad, C.C., A. Akhmanova, F. Grosveld, C.I. De Zeeuw, and N. Galjart. 2000. Functional analysis of CLIP-115 and its binding to microtubules. J. Cell Sci. 113:2285-2297.
12. Jordan, M.A., R.J. Toso, D. Thrower, and L. Wilson. 1993. Mechanism of mitotic block and inhibition of cell proliferation by taxol at low concentrations. Proc. Natl. Acad. Sci. USA. 90:9552-9556.
13. Kim, S.A., and P. Schwille. 2003. Intracellular applications of fluorescence correlation spectroscopy: prospects for neuroscience. Curr. Opin. Neurobiol. 13:583-590.
14. Komarova, Y.A., A.S. Akhmanova, S. Kojima, N. Galjart, and G.G. Borisy. 2002. Cytoplasmic linker proteins promote microtubule rescue in vivo. J. Cell Biol. 159:589-599.
15. Komarova, Y., G. Lansbergen, N. Galjart, F. Grosveld, G.G. Borisy, and A. Akhmanova. 2005. EB1 and EB3 control CLIP dissociation from the ends of growing microtubules. Mol. Biol. Cell. 16:5334-5345.
16. Lansbergen, G., and A. Akhmanova. 2006. Microtubule plus end: a hub of cellular activities. Traffic. 7:499-507.
17. Lansbergen, G., Y. Komarova, M. Modesti, C. Wyman, C.C. Hoogenraad, H.V. Goodson, R.P. Lemaitre, D.N. Drechsel, E. van Munster, T.W. Gadella Jr., et al. 2004. Conformational changes in CLIP-170 regulate its binding to microtubules and dynactin localization. J. Cell Biol. 166:1003-1014.
18. Lansbergen, G., I. Grigoriev, Y. Mimori-Kiyosue, T. Ohtsuka, S. Higa, I. Kitajima, J. Demmers, N. Galjart, A.B. Houtsmuller, F. Grosveld, and A. Akhmanova. 2006. CLASPs attach microtubule plus ends to the cell cortex through a complex with LL5beta. Dev. Cell. 11:21-32.
19. Lele, T.P., and D.E. Ingber. 2 006. A mathematical model to determine molecular kinetic rate constants under non-steady state conditions using fluorescence recovery after photobleaching (FRAP). Biophys. Chem. 120:32-35.
20. Mitchison, T., and M. Kirschner. 1984. Dynamic instability of microtubule growth. Nature. 312:237-242.
21. Muller, J.D. 2004. Cumulant analysis in fluorescence fluctuation spectroscopy. Biophys. J. 86:3981-3992.
22. Perez, F., G.S. Diamantopoulos, R. Stalder, and T.E. Kreis. 1999. CLIP-170 highlights growing microtubule ends in vivo. Cell. 96:517-527.
23. Peris, L., M. Thery, J. Faure, Y. Saoudi, L. Lafanechere, J.K. Chilton, P. Gordon-Weeks, N. Galjart, M. Bornens, L. Wordeman, et al. 2006. Tubulin tyrosination is a major factor affecting the recruitment of CAP-Gly proteins at microtubule plus ends. J. Cell Biol. 174:839-849.
24. Pierre, P., J. Scheel, J.E. Rickard, and T.E. Kreis. 1992. CLIP-170 links endocytic vesicles to microtubules. Cell. 70:887-900.
25. Schuyler, S.C., and D. Pellman. 2001. Microtubule "plus-end-tracking proteins" : the end is just the beginning. Cell. 105:421-424.
26. Seiffert, S., and W. Oppermann. 2005. Systematic evaluation of FRAP experiments performed in a confocal laser scanning microscope. J. Microsc. 220:20-30.
27. Slep, K.C., and R.D. Vale. 2007. Structural basis of microtubule plus end tracking by XMAP215, CLIP-170, and EB1. Mol. Cell. 27:976-991.
28. Stepanova, T., J. Slemmer, C.C. Hoogenraad, G. Lansbergen, B. Dortland, C.I. De Zeeuw, F. Grosveld, G. van Cappellen, A. Akhmanova, and N. Galjart. 2003. Visualization of microtubule growth in cultured neurons via the use of EB3-GFP (end-binding protein 3-green fluorescent protein). J. Neurosci. 23:2655-2664.
29. Wachsmuth, M., T. Weidemann, G. Muller, U.W. Hoffmann-Rohrer, T.A. Knoch, W. Waldeck, and J. Langowski. 2003. Analyzing intracellular binding and diffusion with continuous fluorescence photobleaching. Biophys. J. 84:3353-3363.
30. Wittmann, T., and C.M. Waterman-Storer. 2005. Spatial regulation of CLASP affinity for microtubules by Rac1 and GSK3β in migrating epithelial cells. J. Cell Biol. 169:929-939.