A mathematical model to determine molecular kinetic rate constants under non-steady state conditions using fluorescence recovery after photobleaching (FRAP)

Biophysical Chemistry

Volumn 120, Issue 1, 2006, Pages 32-35

  Lele, Tanmay P ^a   Ingber, Donald E ^a  

^a BOSTON CHILDREN S HOSPITAL   (United States)

Author keywords

Adhesion; Binding kinetics; FRAP; Photobleaching; Unsteady

Indexed keywords

CYTOSKELETON PROTEIN; NUCLEAR MATRIX PROTEIN; SCAFFOLD PROTEIN;

ARTICLE; BINDING AFFINITY; BINDING KINETICS; CELL NUCLEUS MATRIX; CELL STRUCTURE; CYTOSKELETON; ENZYME KINETICS; FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; FOCAL ADHESION; MATHEMATICAL MODEL; MOLECULAR DYNAMICS; MOLECULAR INTERACTION; MOLECULAR MODEL; PRIORITY JOURNAL; STEADY STATE; TRANSCRIPTION INITIATION;

FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; KINETICS; MODELS, THEORETICAL; PROTEINS;

EID: 32844470047     PISSN: 03014622     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bpc.2005.10.007     Document Type: Article

References (21)

1. D. Axelrod, D. Koppel, J. Schlessinger, E. Elson, and W. Webb Mobility measurement by analysis of fluorescence photobleaching recovery kinetics Biophys. J. 16 1976 1055 1069
2. C. Ballestrem, B. Hinz, B.A. Imhof, and B. Wehrle-Haller Marching at the front and dragging behind: differential alphaVbeta3-integrin turnover regulates focal adhesion behavior J. Cell Biol. 155 2001 1319 1332
3. K.A. Beningo, M. Dembo, I. Kaverina, J.V. Small, and Y.L. Wang Nascent focal adhesions are responsible for the generation of strong propulsive forces in migrating fibroblasts J. Cell Biol. 153 2001 881 888
4. A.D. Bershadsky, N.Q. Balaban, and B. Geiger Adhesion-dependent cell mechanosensitivity Annu. Rev. Cell Dev. Biol. 19 2003 677 695
5. J.C. Bulinski, D.J. Odde, B.J. Howell, T.D. Salmon, and C.M. Waterman-Storer Rapid dynamics of the microtubule binding of ensconsin in vivo J. Cell Sci. 114 2001 3885 3897
6. M. Dundr, U. Hoffmann-Rohrer, Q. Hu, I. Grummt, L. Rothblum, R. Phair, and T. Misteli A kinetic framework for a mammalian RNA polymerase in vivo Science 298 2002 1538
7. E.N. Kaufman, and R.K. Jain Quantification of transport and binding parameters using fluorescence recovery after photobleaching Biophys. J. 58 1990 873 885
8. E.N. Kaufman, and R.K. Jain Measurement of mass transport and reaction parameters in bulk solution using photobleaching Biophys. J. 60 1991 596 608
9. T. Lele, J. Pendse, S. Kumar, M. Salanga, J. Karavitis, D. Ingber. Mechanical forces alter zyxin unbinding kinetics within focal adhesions of living cells. J. Cellular Physiology (in press).
10. T.P. Lele, P. Oh, J. Nickerson, and D. Ingber An improved mathematical approach for determination of molecular kinetics in living cells with FRAP Mech. Chem. Biosyst. 1 2004 181 190
11. N.M. McKenna, J.B. Meigs, and Y.L. Wang Exchangeability of alpha-actinin in living cardiac fibroblasts and muscle cells J. Cell Biol. 101 1985 2223 2232
12. R. Phair, and T. Misteli High mobility of proteins in the mammalian cell nucleus Nature 404 2000 604 609
13. R. Phair, and T. Misteli Kinetic modelling approaches to in vivo imaging Nat. Rev., Mol. Cell Biol. 2 2001 898 907
14. B.L. Sprague, R.L. Pego, D.A. Stavreva, and J.G. McNally Analysis of binding reactions by fluorescence recovery after photobleaching Biophys. J. 86 2004 3473 3495
15. M.S. Steinberg, and P.M. McNutt Cadherins and their connections: adhesion junctions have broader functions Curr. Opin. Cell Biol. 11 1999 554 560
16. D.L. Stenoien, M. Mielke, and M.A. Mancini Intranuclear ataxin1 inclusions contain both fast- and slow-exchanging components Nat. Cell Biol. 4 2002 806 810
17. D.L. Stenoien, A.C. Nye, M.G. Mancini, K. Patel, M. Dutertre, B.W. O'Malley, C.L. Smith, A.S. Belmont, and M.A. Mancini Ligand-mediated assembly and real-time cellular dynamics of estrogen receptor alpha-coactivator complexes in living cells Mol. Cell Biol. 21 2001 4404 4412
18. D.L. Stenoien, K. Patel, M.G. Mancini, M. Dutertre, C.L. Smith, B.W. O'Malley, and M.A. Mancini FRAP reveals that mobility of oestrogen receptor-alpha is ligand- and proteasome-dependent Nat. Cell Biol. 3 2001 15 23
19. J.P. Th'ng, R. Sung, M. Ye, and M.J. Hendzel H1 family histones in the nucleus. Control of binding and localization by the C-terminal domain J. Biol. Chem. 280 2005 27809 27814
20. G. von Wichert, B. Haimovich, G.S. Feng, and M.P. Sheetz Force-dependent integrin-cytoskeleton linkage formation requires downregulation of focal complex dynamics by Shp2 EMBO J. 22 2003 5023 5035
21. R. Zaidel-Bar, C. Ballestrem, Z. Kam, and B. Geiger Early molecular events in the assembly of matrix adhesions at the leading edge of migrating cells J. Cell Sci. 116 2003 4605 4613