Lactation failure in Src knockout mice is due to impaired secretory activation

BMC Developmental Biology

Volumn 8, Issue , 2008, Pages

  Watkin, Harriet ^a   Richert, Monica M ^a,c   Lewis, Andrew ^a   Terrell, Kristina ^a   McManaman, James P ^a,b   Anderson, Steven M ^a  

^a UNIVERSITY OF COLORADO   (United States)

^b UNIVERSITY OF COLORADO SCHOOL OF MEDICINE   (United States)

^c University of Alabama at Birmingham   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MILK PROTEIN; PROLACTIN RECEPTOR; PROTEIN TYROSINE KINASE; RNA; STAT5 PROTEIN; CASEIN; PROLACTIN; STAT5A PROTEIN, MOUSE; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BIRTH; FEMALE; GENE; GENE EXPRESSION; INVOLUTION; KNOCKOUT MOUSE; LACTATION DISORDER; MAMMARY GLAND FUNCTION; MILK PROTEIN GENE; MOUSE; NONHUMAN; ORGANOGENESIS; PREGNANCY; PROLACTIN BLOOD LEVEL; PROTEIN PHOSPHORYLATION; RNA TRANSLATION; SECRETION; ANIMAL; BLOOD; C57BL MOUSE; CELL CULTURE; FLUORESCENT ANTIBODY TECHNIQUE; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; HISTOLOGY; LACTATION; MOUSE MUTANT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; SUCKLING; UDDER; WEIGHT GAIN;

MUS;

ANIMALS; ANIMALS, SUCKLING; CASEINS; CELLS, CULTURED; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE; GENE EXPRESSION REGULATION, DEVELOPMENTAL; LACTATION; MAMMARY GLANDS, ANIMAL; MICE; MICE, INBRED C57BL; MICE, KNOCKOUT; PREGNANCY; PROLACTIN; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; SRC-FAMILY KINASES; STAT5 TRANSCRIPTION FACTOR; WEIGHT GAIN;

EID: 40749130891     PISSN: None     EISSN: 1471213X     Source Type: Journal    
DOI: 10.1186/1471-213X-8-6     Document Type: Article

References (92)

1. The cues that regulate ductal branching and morphogenesis. MD Sternlicht, Breast Cancer Res 2006 8 201 212 16524451 10.1186/bcr1368
2. Insights from the study of animals lacking functional estrogen receptor. KS Korach, Science 1994 266 1524 1527 10.1126/science.7985022 7985022
3. Estrogen receptor gene disruption: molecular characterization and experimental and clinical phenotypes. KS Korach JF Couse SW Curtis TF Washburn J Lindzey, Recent Prog Horm Res 1996 51 159 186 8701078
4. Prolactin Regulation of Mammary Gland Development. SR Oakes RL Rogers MJ Naylor CJ Ormandy, J Mammary Gland Biol Neoplasia 2008 18219564 18219564
5. Mammary gland development is mediated by both stromal and epithelial progesterone receptors. RC Humphreys JP Lydon BW O'Malley J Rosen, Mol Endo 1997 11 801 811 10.1210/me.11.6.801
6. Use of PRKO mice to study the role of progesterone in mammary gland development. RC Humphreys JP Lydon BW O'Malley J Rosen, J Mam Gland Bio Neo 1997 2 343 354 10.1023/A:1026343212187
7. Functional development of the mammary gland: Use of expression profiling and trajectory clustering to reveal changes in gene expression during pregnancy, lactation, and involution. MC Rudolph JL McManaman L Hunter T Phang MC Neville, J Mam Gland Bio Neo 2003 8 287 307 10.1023/B:JOMG.0000010030.73983.57
8. Metabolic regulation in the lactating mammary gland: A lipid synthesizing machine. MC Rudolph JL McManaman T Phang T Russel DM Kominsky N Serkova SM Anderson MC Neville, Physiol Genomics 2006 28 323 336 10.1152/physiolgenomics. 00020.2006 17105756
9. Key stages in mammary gland development - The alveolar switch: coordinating the proliferative cues and cell fate decisions that drive the formation of lobuloalveoli from ductal epithelium. S Oakes H Hilton C Ormandy, Breast Cancer Research 2006 8 207 16677418 10.1186/bcr1411
10. Secretory activation in the mammary gland: it's not just about milk protein synthesis. SM Anderson MC Rudolph JL McManaman MC Neville, Breast Cancer Res 2007 9 204 217 17338830 10.1186/bcr1653
11. Progesterone withdrawal stimulates mammary gland tubulin polymerization in pregnant rats. RF Loizzi, Endocrinology 1985 116 2543 2547 2986954
12. Lactogenesis induced by ovariectomy in pregnany rats and its regulation by oestrogen and progesterone. RP Deis C Delouis, J Steroid Biochem 1983 18 687 690 10.1016/0022-4731(83)90246-7 6408313
13. Progesterone-mediated inhbition of casein mRNA and polysomal casein synthesis in the rat mammary gland during pregnancy. JM Rosen DL O'Neal JE McHugh JP Comstock, Biochemistry 1978 17 290 297 10.1021/bi00595a016 619992
14. Hormonal regulation of tight junction closure in the mouse mammary epithelium during the transition from pregnancy to lactation. D-A Nguyen AF Parlow MC Neville, Endocrinology 2001 170 347 356 10.1677/joe.0.1700347
15. Expression of constitutively activated Akt in the mammary gland leads to excess lipid synthesis during pregnancy and lactation. KL Schwertfeger JL McManaman CA Palmer MC Neville SM Anderson, J Lipid Res 2003 44 1100 1112 10.1194/jlr.M300045-JLR200 12700340
16. Socs2 and Elf5 Mediate Prolactin-Induced Mammary Gland Development. J Harris PM Stanford K Sutherland SR Oakes MJ Naylor FG Robertson KD Blazek M Kazlauskas HN Hilton S Wittlin, Mol Endo 2006 20 1177 1187 10.1210/me.2005-0473
17. The prolactin/growth hormone receptor family. PA Kelly J Djiane M-C Postel-Vinay M Edery, Endocrine Rev 1991 12 235 251
18. Prolactin-mediated transcriptional and post-transcriptional control of casein gene expression. WA Guyette RJ Matusik JM Rosen, Cell 1979 17 1013 1023 10.1016/0092-8674(79)90340-4 487427
19. Mammary plasminogen activator: Correlation with involution, hormonal modulation and comparison, between normal and neoplastic. L Ossowski D Biegel EC Reich, Cell 1979 16 929 940 10.1016/0092-8674(79)90108-9 455456
20. Key stages in mammary gland development - Involution: apoptosis and tissue remodelling that convert the mammary gland from milk factory to a quiescent organ. C Watson, Breast Cancer Research 2006 8 203 16677411 10.1186/bcr1401
21. The src oncogene. A Golden JS Brugge, The Oncogene Handbook New York, NY: Elsevier, Reddy EP, Skalka AM, Curran T, 1 1988 149 174
22. Dynamic coupling between the SH2 and SH3 domains of c-src and hck underlies their inactivation by C-terminal tyrosine phosphorylation. MA Young S Gonflori G Superti-Furga B Roux J Kuriyan, Cell 2001 105 115 126 10.1016/S0092-8674(01)00301-4 11301007
23. Vaiation on an Src-like theme. SC Harrison, Cell 2003 112 737 740 10.1016/S0092-8674(03)00196-X 12654240
24. Activation of src family kinases by colony-stimulating factor-1, and their association with its receptor. SA Courtneidge R Dhand D Pilat GM Twamley MD Waterfield MF Roussel, EMBO J 1993 12 943 950 7681396
25. A novel inhibitor of cyclin-cdk activity detected in transforming grwoth factor B-arrested epithelial cells. JM Slingerland L Hengst C-H Pan D Alexander MR Stampfer SI Reed, Mol Cell Biol 1994 14 3683 3694 8196612
26. The src family tyrosine kinases are required for platelet-derived growth factor-mediated signal transduction in NIH 3T3 cells. GM Twamley-Stein R Pepperkok W Ansorge SA Courtneidge, Proc Natl Acad Sci USA 1993 90 7696 7700 8356071 10.1073/pnas.90.16.7696
27. Progesterone receptor contains a proline-rich motif that directly interacts wtih SH3 domains and activates c-Src family tyrosine kinases. V Boonyaratanakornkit MP Scott V Ribon L Sherman SM Anderson JL Maller WT Miller DP Edwards, Molecular Cell 2001 8 269 280 10.1016/S1097-2765(01)00304-5 11545730
28. Two domains of the progesterone receptor interact with the estrogen receptor and are required for progesterone activation of the c-Src/Erk pathway in mammalian cells. C Ballaré M Uhring T Bechtold E Sancho M Di Domenico A Migliaccio F Auricchio M Beato, Mol Cell Biol 2003 23 1994 2008 12612073 10.1128/MCB.23.6.1994-2008.2003
29. Characterization of the Interactions of Estrogen Receptor and MNAR in the Activation of cSrc. F Barletta CW Wong C McNally BS Komm B Katzenellenbogen BJ Cheskis, Mol Endo 2004 18 1096 1108 10.1210/me.2003-0335
30. Cellular functions regulated by Src family kinases. SM Thomas JS Brugge, Annu Rev Cell Dev Biol 1997 13 513 609 10.1146/annurev.cellbio.13.1.513 9442882
31. c-Src-null mice exhibit defects in normal mammary gland development and ERα signaling. H Kim M Laing W Muller, Oncogene 2005 5629 5636 10.1038/sj.onc.1208718 16007215
32. Lactation and its hormonal control. MC Neville, Knobil and Neils Physiology of Reproduction New York: Elsevier, Neill JD, Third 2006 2993 3053
33. Protein kinase expression during murine mammary development. LA Chodosh HP Gardner JV Rajan DB Stairs ST Marquis PA Leder, Developmental Biology 2000 219 259 276 10.1006/dbio.2000.9614 10694421
34. Claudin 7 expression and localization in the normal murine mammary gland and murine mammary tumors. B Blackman T Russell S Nordeen D Medina M Neville, Breast Cancer Res 2005 7 R248 R255 15743505 10.1186/bcr988
35. Defective mammary gland morphogenesis in mice lacking the progesterone receptor B isoform. B Mulac-Jericevic JP Lydon FJ DeMayo OM Conneely, Proceedings of the National Academy of Sciences 2003 100 9744 9749 10.1073/pnas.1732707100
36. The aqueous phase of milk: ion and water transport. M Peaker, Symposia of the Zoological Society of London 1977 41 113 134
37. An atlas of mouse mammary gland development. MM Richert KL Schwertfeger JW Ryder SM Anderson, J Mam Gland Bio Neo 2000 5 227 241 10.1023/A:1026499523505
38. Regulation of milk lipid formation and secretion in the mouse mammary gland. JL McManaman CA Palmer S Anderson K Schwertfeger MC Neville, Adv Exp Med Biol 2004 554 263 279 15384582
39. Mammary gland development. CJ Ormandy ND Horseman MJ Naylor J Harris F Robertson N Binart PA Kelly, Prolactin Boston: Klumer Academic Publishers, Horseman ND, 1 2001 219 232
40. The role pf proloactin and growth hormone in mammary gland development. PA Kelly A Bachelot C Kedzia L Hennighausen CJ Ormandy JJ Kopchick N Binart, Mol Cell Endo 2002 197 127 131 10.1016/S0303-7207(02)00286-1
41. From the molecular biology of prolactin and its receptor to the lessons learned from knockout mice models. V Goffin N Binart P Clement-Lacroix B Bouchard C Bole-Feysot M Edery BK Lucas P Touraine A Pezet R Maaskant, Genetic Analysis 1999 15 189 201 10596761
42. Transcriptional and spatiotemporal regulation of prolactin receptor mRNA and cooperativity with progesterone receptor function during ductal branch growth in the mammary gland. RC Hovey JF Trott E Ginsburg A Goldhar MM Sasaki SJ Fountain K Sundararajan BK Vonderhaar, Developmental Dynamics 2001 222 192 205 10.1002/dvdy.1179 11668597
43. Prolactin induces phosphorylation of Tyr694 of STAT5 (MGF), a prerequisite for DNA binding and induction of transcription. F Gouilleux H Wakao V Mundt B Groner, EMBO J 1994 13 4361 4369 7925280
44. Cloning and expression of Stat5 and an additional homologue (Stat5b) involved in prolactin signal transduction in mouse mammary tissue. X Liu GW Robinson F Gouilleux B Groner L Hennighausen, Proc Natl Acad Sci USA 1995 92 8831 8835 7568026 10.1073/pnas.92.19.8831
45. Mammary gland factor (MGF) is a novel member of the cytokine regulated transcription factor gene family and confers the prolactin response. H Wakao F Gouilleux B Groner, EMBO J 1994 13 2182 2191 7514531
46. Activation of Stat5a and Stat5b by tyrosine phosphorylation is tightly linked to mammary gland differentiation. X Liu GW Robinson L Hennighausen, Mol Endo 1996 10 1496 1506 10.1210/me.10.12.1496
47. Apoptotic cell death and tissue remodeling during mouse mammry gland involution. R Strange F Li S Saurer A Burkhardt RR Friis, Development 1992 115 49 58 1638991
48. Cell death by apoptosis during involution of the lactating breast in mice and rats. NI Walker RE Bennett JFR Kerr, Am Anat 1989 185 19 32 10.1002/aja.1001850104
49. Inability of overexpression des(1-3) human insulin-like growth factor I (IGF-I) to inhibit forced mammary gland involution is associated wtih decreased expression of IGF signaling molecules. DL Hadsell T Alexeenko Y Klemintidis D Torres AV Lee, Endocrinology 2001 142 1479 1488 10.1210/en.142.4.1479 11250928
50. Suppression of epithelial apoptosis and delayed mammary gland involution in mice with a conditional knockout of Stat3. RS Chapman PC Lourenco E Tonner DJ Flint S Selbert K Takeda S Akira AR Clarke CJ Watson, Genes and Dev 1999 13 2604 2616 10.1101/gad.13.19.2604
51. Developmental regulation of Bcl-2 family protein expression in the involuting mammary gland. AD Metcalfe A Gilmore T Klinowska J Oliver AJ Valentijn R Brown A Ross G MacGregor JA Hickman CH Streuli, J Cell Sci 1999 112 1771 1783 10318769
52. Programmed cell death and the mammary gland- the involvement of the Bcl-2 family members in the control of epithelial apoptosis. AD Metcalfe JA Hickman CH Streuli, Biochemical Society Transactions 1998 24 347S
53. Gain of Bcl-2 is more potent than Bax loss in regulating mammary epithelial cell survival in vivo. K Schoor M Li U Bar-Peled A Lewis A Heredia B Lewis CM Knudson SJ Korsmeyer R Jager H Weiher, Cancer Res 1999 59 2541 2545 10363969
54. Coordinated expression of extracellular matrix-degrading proteinases and their inhibitors regulates mammary epithalial function during involution. RS Talhouk MJ Bissell Z Werb, J Cell Biol 1992 118 1271 1282 10.1083/jcb.118.5.1271 1512297
55. Deletion of Stat3 blocks mammary gland involution and extends functional competence of the secretory epithelium in the absence of lactogenic stimuli. RC Humphreys B Bierie Z Zhao R Raz D Levy L Hennighausen, Endocrinology 2002 143 3641 3650 10.1210/en.2002-220224 12193580
56. Knockouts of Src-family kinses: Stiff bones, wimpy T cells, and bad memory. CA Lowell P Soriano, Genes and Dev 1996 10 1845 1857 10.1101/gad.10.15.1845
57. Unpublished data. MM Richert
58. Targeted disruption of the c-src proto-oncogene leads to osteopetrosis in mice. P Soriano C Montgomery R Geske A Bradley, Cell 1991 64 693 702 10.1016/0092-8674(91)90499-O 1997203
59. Null mutation of the prolactin receptor gene produces multiple reproductive defects in the mouse. CJ Ormandy A Camus J Barra D Damotte B Lucas H Buteau M Edery N Brousse C Babinet N Binart, Genes and Dev 1997 11 167 178 10.1101/gad.11.2.167
60. Isoform-specific requirement for Akt1 in the developmental regulation of cellular metabolism during lactation. RB Boxer DB Stairs KD Dugan KL Notarfrancesco CP Portocarrero BA Keister GK Belka H Cho JC Rathmell CB Thompson, Cell Metabolism 2006 4 475 490 10.1016/j.cmet.2006.10.011 17141631
61. Stat5 as a target for regulation by extracellular matrix. CH Streuli GM Edwards M Delcommenne CBA Whitelaw TG Burdon C Schindler CJ Watson, J Biol Chem 1995 270 21639 21644 10.1074/jbc.270.45.26794 7665578
62. Rac1 links integrin-mediated adhesion to the control of lactational differentiation in mammary epithelia. N Akhtar CH Streuli, The Journal of Cell Biology 2006 173 781 793 16754961 10.1083/jcb.200601059
63. A role for the cytoskeleton in prolactin-dependent mammary epithelial cell differentiation. GS Zoubiane A Valentijn ET Lowe N Akhtar S Bagley AP Gilmore CH Streuli, J Cell Sci 2004 117 271 280 10.1242/jcs.00855 14676278
64. pp125FAK, a structurally distinctive protein-tyrosine kinase associated with focal adhesions. MD Schaller CA Borgman BS Cobb RR Vines AB Reynolds JT Parsons, Proc Natl Acad Sci USA 1992 89 5192 5196 1594631 10.1073/pnas.89.11. 5192
65. Cell adhesion or integrin clustering increases phosphoryation of a focal adhesion-associated tyrosine kinase. L Kornberg HS Earp JT Parsons M Schaller RL Juliano, J Biol Chem 1992 267 23439 23442 1429685
66. Tyrosine phosphorylation of paxillin and pp125FAK accompanies adhesion to extracellular matrix: A role in cytoskeletal assembly. K Burridge CE Turner LH Romer, J Cell Biol 1992 119 893 903 10.1083/jcb.119.4.893 1385444
67. Fibronectin/integrin interaction induces tyrosine phosphorylation of a 120 kDa protein. J-L Guan JE Trevithick RO Hynes, Cell Reg 1991 2 951 964
68. Tyrosine phosphorylation of focal adhesion kinase at sites in the catalytic domain regulates kinase activity: a role for src fmaily kinase. MB Calalb TR Polte SK Hanks, Mol Cell Biol 1995 15 954 963 7529876
69. Autophosphorylation of the focal adhesion kinase, pp125FAK, directs SH-2dependent biding of pp60src. MD Schaller JD Hildebrand JD Shannon JW Fox RR Vines JT Parsons, Mol Cell Biol 1994 14 1680 1688 7509446
70. Stable association of pp60src and p59fyn with the focal adhesion-associated protein kinase, pp125 FAK. BS Cobb MD Schaller T-H Leu JT Parsons, Mol Cell Biol 1994 14 147 155 7505391
71. SH2- and SH3-mediated intereactions between focal adhesion kinase and src. JW Thomas B Ellis RJ Boerner WB KNight JM White MD Schaller, J Biol Chem 1998 273 577 583 10.1074/jbc.273.1.577 9417118
72. Tyrosine phosphorylated Par3 regulates epithelial tight junction assembly promoted by EGFR signaling. Y Wang D Du L Fang G Yang C Zhang R Zeng A Ullrich F Lottspeich Z CHen, EMBO J 2006 25 5058 5070 17053785 10.1038/sj.emboj.7601384
73. The c-Src tyrosine kinase associates with the catalytic domain of ErbB-2: implications for ErbB-2 mediated signaling and transformation. H Kim R Chan DL Dankort D Zuo M Najoukas M Park WJ Muller, Oncogene 2005 24 7599 7607 10.1038/sj.onc.1208898 16170374
74. GTP-binding proteins of the Rho/Rac family: regulation, efectors and functions in vivo. XR Bustelo V Sauzeau IM Berenjeno, BioEssays 2007 29 356 370 17373658 10.1002/bies.20558
75. Rac1 Function Is Required for Src-induced Transformation: EVIDENCE OF A ROLE FOR TIAM1 AND VAV2 IN RAC ACTIVATION BY SRC. JM Servitja MJ Marinissen A Sodhi XR Bustelo JS Gutkind, Journal of Biological Chemistry 2003 278 34339 34346 10.1074/jbc.M302960200 12810717
76. Vav2 as a Rac-GDP/GTP Exchange Factor Responsible for the Nectin-induced, c-Src- and Cdc42-mediated Activation of Rac. T Kawakatsu H Ogita T Fukuhara T Fukuyama Y Minami K Shimizu Y Takai, Journal of Biological Chemistry 2005 280 4940 4947 10.1074/jbc.M408710200 15485841
77. Caveolin-1 regulates cell polarization and directional migration through Src kinase and Rho GTPases. A Grande-Garcia A Echarri J de Rooij NB Alderson CM Waterman-Storer JM Valdivielso MA del Pozo, The Journal of Cell Biology 2007 177 683 694 17517963 10.1083/jcb.200701006
78. DIP (mDia ineracting protein) is a key moleculae regulating Rho and Tac in a Src-dependent manner. W Meng M Numazaki K Takeuchi Y Uchibori Y Ando-Akatsuka M Tominaga T Tominaga, EMBO J 2004 23 760 771 14765113 10.1038/sj.emboj.7600095
79. Rac1 links integrin-mediated adhesion to the control of lactational differentiation in mammary epithelia. N Akhtar CH Streuli, The Journal of Cell Biology 2006 173 781 793 16754961 10.1083/jcb.200601059
80. Impaired tight junction sealing and precocious involution in mammary glands of PKN1 transgenic mice. A Fischer H Stuckas M Gluth TD Russell MC Rudolph NE Beeman S Bachmann S Umemura Y Ohashi MC Neville, J Cell Sci 2007 120 2272 2283 10.1242/jcs.03467 17591691
81. Milk accumulation triggers apoptosis of mammary epithelial cells. A Marti Z Feng HJ Altermatt R Jaggi, Eur J Cell Biol 1997 73 158 165 9208229
82. A dual, non-redundant, role for LIF as a regulator of development and STAT3-mediated cell death in mammary gland. EA Kritikou A Sharkey K Abell PJ Came E Anderson RW Clarkson CJ Watson, Development 2003 130 3459 3468 10.1242/dev.00578 12810593
83. Loss of interleukin 6 results in delayed mammary gland involution: A possible role for mitogen-activated protein kinase and not signal transducer and activator of transcription 3. L Zhao JJ Melenhorst L Hennighausen, Mol Endocrinol 2002 16 2902 2912 10.1210/me.2001-0330 12456808
84. Roles of Fas and Fas ligand during mammary gland remodeling. J Song E Sapi W Brown J Nilsen K Tartaro BM Kacinski J Craft F Naftolin G Mor, J Clin Invest 2000 106 1209 1220 11086022 10.1172/JCI10411
85. Cell-matrix intercations during development and apoptosis o fhte mouse mammary gland in vivo. JM Prince TC Klinowska E Marshman ET Lowe U Mayer D Aberdam D Vestweber B Gusterson CH Streuli, Developmental Dynamics 2002 223 497 516 10.1002/dvdy.10070 11921338
86. Transforming growth factor beta3 induces cell death during the first stage of mammary gland involution. AV Nguyen JW Pollard, Development 2000 127 3107 3118 10862748
87. Insulin-like growth factor binding protein-5 (IGFBP-5) induces premature cell death in the mammary glands of transgenic mice. E Tonner MC Barber GJ Allan J Beattie J Webster CBA Whitelaw DJ Flint, Development 2002 129 4547 4557 12223411
88. Mammary gland involution is delayed by activated Akt in transgenic mice. KL Schwertfeger MM Richert SM Anderson, Mol Endocrinol 2001 15 867 881 10.1210/me.15.6.867 11376107
89. Transcriptional regulation of the lung fatty acid synthase gene by glucocorticoid, thyroid hormone and transforming growth factor-β1. Z Lu Y Gu SA Rooney, BBA 2001 1532 213 222 11470242
90. Elvax 40P implants:sustained, local release of bioactive molecules influencing mammary ductal development. GB Silberstein CW Daniel, Developmental Biology 1982 93 272 278 10.1016/0012-1606(82)90259-7 7128936
91. The insulin-like growth factors (IGF) and IGF type I receptor during postnatal growth of the murine mammary gland: Sites of messenger ribonculeic acid expression and potential function. MM Richert TL Wood, Endocrinology 1999 140 454 461 10.1210/en.140.1.454 9886857
92. The mammary gland epithelial cell. S Pullan CH Streuli, Epithelial Cell Culture Cambridge: Cambridge University Press, Harris A, 1996 97 121