The mucin degrader Akkermansia muciniphila is an abundant resident of the human intestinal tract

Applied and Environmental Microbiology

Volumn 74, Issue 5, 2008, Pages 1646-1648

  Derrien, Muriel ^a   Collado, M Carmen ^b   Ben Amor, Kaouther ^a   Salminen, Seppo ^b   De Vos, Willem M ^a,c  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b UNIVERSITY OF TURKU   (Finland)

^c UNIVERSITY OF HELSINKI   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; CELLS; RNA;

AKKERMANSIA MUCINIPHILA; INTESTINAL TRACT; MUCIN DEGRADER;

PROBES;

MUCIN; RNA 16S;

BACTERIUM; BIODEGRADATION; DESIGN; FECAL PELLET; FLUORESCENCE; GENE; HYBRIDIZATION; IN SITU TEST; MUCILAGE; PHYSIOLOGY; PROBE; RNA;

AKKERMANSIA MUCINIPHILA; ARTICLE; BACTERIAL CELL; BACTERIUM; BIODEGRADABILITY; FECES ANALYSIS; FLUORESCENCE IN SITU HYBRIDIZATION; INTESTINE; INTESTINE FLORA; NONHUMAN; NUCLEOTIDE SEQUENCE; RNA PROBE; VALIDATION PROCESS;

BACTERIA; COMPUTATIONAL BIOLOGY; DNA PRIMERS; FECES; FLOW CYTOMETRY; GASTRIC MUCIN; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; INTESTINES; OLIGONUCLEOTIDE PROBES; PHYLOGENY; RNA, RIBOSOMAL, 16S;

AKKERMANSIA MUCINIPHILA; BACTERIA (MICROORGANISMS);

EID: 40549106737     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.01226-07     Document Type: Article

References (19)

1. Allen, A. 1983. Mucus - a protective secretion of complexity. Trends Biochem. Sci. 8:169-173.
2. Alm, E., D. Oerther, N. Larsen, D. Stahl, and L. Raskin. 1996. The oligonucleotide probe database. Appl. Environ. Microbiol. 62:3557-3559.
3. Amann, R., L. Krumholz, and D. Stahl. 1990. Fluorescent- oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. J. Bacteriol. 172:762-770.
4. Cole, J. R., B. Chai, R. J. Farris, Q. Wang, S. A. Kulam, D. M. McGarrell, G. M. Garrity, and J. M. Tiedje. 2005. The Ribosomal Database Project (RDP-II): sequences and tools for high-throughput rRNA analysis. Nucleic Acids Res. 33:D294-D296.
5. Collado, M. C., M. Derrien, E. Isolauri, W. de Vos, and S. Salminen. 2007. Intestinal integrity and Akkermansia muciniphila, a mucin-degrading member of the intestinal microbiota present in infants, adults, and the elderly. Appl. Environ. Microbiol. 73:7767-7770.
6. Derrien, M., E. E. Vaughan, C. M. Plugge, and W. M. de Vos. 2004. Akkermansia muciniphila gen. nov., sp. nov., a human intestinal mucin-degrading bacterium. Int. J. Syst. Evol. Microbiol. 54:1469-1476.
7. Eckburg, P. B., E. M. Bik, C. N. Bernstein, E. Purdom, L. Dethlefsen, M. Sargent, S. R. Gill, K. E. Nelson, and D. A. Relman. 2005. Diversity of the human intestinal microbial flora. Science 308:1635-1638.
8. Hayashi, H., M. Sakamoto, and Y. Benno. 2002. Fecal microbial diversity in a strict vegetarian as determined by molecular analysis and cultivation. Microbiol. Immunol. 46:819-831.
9. Hedlund, B. P., J. Gosink, and J. Staley. 1997. Verrucomicrobia div. nov., a new division of the Bacteria containing three new species of Prosthecobacter. Antonie van Leeuwenhoek 72:29-38.
10. Hold, G. L., S. E. Pryde, V. J. Russell, E. Furrie, and H. J. Flint. 2002. Assessment of microbial diversity in human colonic samples by 16S rDNA sequence analysis. FEMS Microbiol. Lett. 39:33-39.
11. Loy, A., M. Horn, and M. Wagner. 2003. probeBase: an online resource for rRNA-targeted oligonucleotide probes. Nucleic Acids Res. 31:514-516.
12. Mangin, I., R. Bonnet, P. Seksik, L. Rigottier-Gois, M. Sutren, Y. Bouhnik, C. Neut, M. D. Collins, J.-F. Colombel, P. Marteau, and J. Dore. 2004. Molecular inventory of faecal microflora in patients with Crohn's disease. FEMS Microbiol. Ecol. 50:25-36.
13. Nelson, K. E., S. H. Zinder, I. Hance, P. Burr, D. Odongo, D. Wasawo, A. Odenyo, and R. Bishop. 2003. Phylogenetic analysis of the microbial populations in the wild herbivore gastrointestinal tract: insights into an unexplored niche. Environ. Microbiol. 5:1212-1220.
14. Rajilić-Stojanović, M. 2007. Diversity of the human gastro-intestinal microbiota: novel perspectives from high throughput analyses. Wageningen University, Wageningen, The Netherlands.
15. Salyers, A. A. 1977. Fermentation of mucins and plant polysaccharides by anaerobic bacteria from the human colon. Appl. Environ. Microbiol. 34:529-533.
16. Salzman, N. H., H. de Jong, Y. Paterson, H. J. M. Harmsen, G. W. Welling, and N. A. Bos. 2002. Analysis of 16S libraries of mouse gastrointestinal microflora reveals a large new group of mouse intestinal bacteria. Microbiology 148:3651-3660.
17. Suau, A., R. Bonnet, M. Sutren, J.-J. Godon, G. R. Gibson, M. D. Collins, and J. Dore. 1999. Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Appl. Environ. Microbiol. 65:4799-4807.
18. Wallner, G., R. Amann, and W. Beisker. 1993. Optimizing fluorescent in situ hybridization with rRNA-targeted oligonucleotide probes for flow cytometric identification of microorganisms. Cytometry 14:136-143.
19. Zoetendal, E. G., K. Ben-Amor, H. J. M. Harmsen, F. Schut, A. D. L. Akkermans, and W. M. de Vos. 2002. Quantification of uncultured Ruminococcus obeum-like bacteria in human fecal samples by fluorescent in situ hybridization and flow cytometry using 16S rRNA-targeted probes. Appl. Environ. Microbiol. 68:4225-4232.