Intestinal integrity and Akkermansia muciniphila, a mucin-degrading member of the intestinal microbiota present in infants, adults, and the elderly

Applied and Environmental Microbiology

Volumn 73, Issue 23, 2007, Pages 7767-7770

  Collado, M Carmen ^a   Derrien, Muriel ^b   Isolauri, Erika ^a   De Vos, Willem M ^b,c   Salminen, Seppo ^a  

^a UNIVERSITY OF TURKU   (Finland)

^b WAGENINGEN UNIVERSITY   (Netherlands)

^c UNIVERSITY OF HELSINKI   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENCE MICROSCOPY; MICROSCOPIC EXAMINATION;

16S RRNA; EARLY LIFE; FLUORESCENCE IN SITU HYBRIDIZATION; INTESTINAL TRACTS; MICROBIOTA; REAL-TIME PCR;

MEDICAL IMAGING;

MUCIN; RNA 16S;

BACTERIUM; DIGESTIVE SYSTEM;

ADULT; AGED; AKKERMANSIA MUCINIPHILA; ARTICLE; BACTERIAL COLONIZATION; CONTROLLED STUDY; FECES; FECES ANALYSIS; FLUORESCENCE IN SITU HYBRIDIZATION; GENE SEQUENCE; HUMAN; INFANT; INTESTINE FLORA; MOLECULAR CLONING; NONHUMAN; NORMAL HUMAN; REAL TIME POLYMERASE CHAIN REACTION; BACTERIUM; CLASSIFICATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; INTESTINE; METABOLISM; MICROBIOLOGY; NEWBORN; POLYMERASE CHAIN REACTION;

AKKERMANSIA MUCINIPHILA; BACTERIA (MICROORGANISMS);

ADULT; AGED, 80 AND OVER; BACTERIA; FECES; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; INFANT; INFANT, NEWBORN; INTESTINES; MUCINS; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S;

EID: 38449104720     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.01477-07     Document Type: Article

References (19)

1. Alm, E., D. Oerther, N. Larsen, D. Stahl, and L. Raskin. 1996. The oligonucleotide probe database. Appl. Environ. Microbiol. 62:3557-3559.
2. Amann, R. I., B. J. Binder, R. J. Olson, S. W. Chisholm, R. Devereux, and D. A. Stahl. 1990. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl. Environ. Microbiol. 56:1919-1925.
3. Campieri, M., and P. Gionchetti. 2001. Bacteria as the cause of ulcerative colitis. Gut 48:132-135.
4. Corfield, A. P., D. Carroll, N. Myerscough, and C. S. Probert. 2001. Mucins in the gastrointestinal tract in health and disease. Front. Biosci. 6:D1321-D1327.
5. Derrien, M. 2007. Mucin utilisation and host interactions of the novel intestinal microbe Akkermansia muciniphila. Ph.D. thesis (ISBN 978-90-8504-644-8). Wageningen University, Wageningen, The Netherlands.
6. Derrien, M., E. E. Vaughan, C. M. Plugge, and W. M. de Vos. 2004. Akkermansia muciniphila gen. nov., sp. nov., a human intestinal mucin-degrading bacterium. Int. J. Syst. Evol. Microbiol. 54:1469-1476.
7. Eckburg, P. B., E. M. Bik, C. N. Bernstein, E. Purdom, L. Dethlefsen, M. Sargent, S. R. Gill, K. E. Nelson, and D. A. Relman. 2005. Diversity of the human intestinal microbial flora. Science 308:1635-1638.
8. Gueimonde, M., S. Tolkko, T. Korpimaki, and S. Salminen. 2004. New real-time quantitative PCR procedure for quantification of bifidobacteria in human fecal samples. Appl. Environ. Microbiol. 70:4165-4169.
9. Hayashi, H., M. Sakamoto, and Y. Benno. 2002. Fecal microbial diversity in a strict vegetarian as determined by molecular analysis and cultivation. Microbiol. Immunol. 46:819-831.
10. Hooper, L. V., and J. I. Gordon. 2001. Commensal host-bacterial relationships in the gut. Science 292:1115-1118.
11. Liévin-Le Moal, V., and A. L. Servin. 2006. The front line of enteric host defense against unwelcome intrusion of harmful microorganisms: mucins, antimicrobial peptides, and microbiota. Clin. Microbiol. Rev. 19:315-337.
12. Midtvedt, A., B. Carlstedt-Duke, and T. Midtvedt. 1994. Establishment of a mucin-degrading intestinal microflora during the first two years of human life. J. Pediatr. Gastroenterol. Nutr. 18:321-326.
13. Rigottier-Gois, L., A.-G. Le Bourhis, G. Gramet, V. Rochet, and J. Doré. 2003. Fluorescent hybridization combined with flow cytometry and hybridization of total RNA to analyse the composition of microbial communities in human faeces using 16S rRNA probes. FEMS Microbiol. Ecol. 43:237-245.
14. Salminen, S., and M. Gueimonde. 2005. Gut microbiota in infants between 6 and 24 months of age. Nestle Nutr. Workshop Ser. Pediatr. Program 56:43-51.
15. Suau, A., R. Bonnet, M. Sutren, J.-J. Godon, G. R. Gibson, M. D. Collins, and J. Doré. 1999. Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Appl. Environ. Microbiol. 65:4799-4807.
16. Vaughan, E. E., H. G. Heilig, K. Ben-Amor, and W. M. de Vos. 2005. Diversity, vitality and activities of intestinal lactic acid bacteria and bifidobacteria assessed by molecular approaches. FEMS Microbiol. Rev. 29:477-490.
17. Wang, M., S. Ahrné, B. Jeppsson, and G. Molin. 2005. Comparison of bacterial diversity along the human intestinal tract by direct cloning and sequencing of 16S rRNA genes. FEMS Microbiol. Ecol. 54:219-231.
18. Zoetendal, E. G., A. D. Akkermans, and W. M. de Vos. 1998. Temperature gradient gel electrophoresis analysis of 16S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria. Appl. Environ. Microbiol. 64:3854-3859.
19. Zoetendal, E. G., E. E. Vaughan, and W. M. de Vos. 2006. A microbial world within us. Mol. Microbiol. 59:1639-1650.