Structural genomics on membrane proteins: The MePNet approach

Current Opinion in Drug Discovery and Development

Volumn 7, Issue 3, 2004, Pages 342-346

  Lundstrom, Kenneth ^a  

^a BioXtal   (Switzerland)

Author keywords

Crystallization; G protein coupled receptors; Membrane proteins; Overexpression; Purification; Structural genomics

Indexed keywords

G PROTEIN COUPLED RECEPTOR; MEMBRANE PROTEIN;

CRYSTAL STRUCTURE; CRYSTALLIZATION; DATA BASE; DRUG TARGETING; NONHUMAN; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; REVIEW; STRUCTURAL GENOMICS; ANIMAL; CHEMISTRY; DRUG DESIGN; GENE VECTOR; GENETICS; GENOMICS; HUMAN; METABOLISM; METHODOLOGY; PROTEIN CONFORMATION; PROTEIN DATABASE;

ANIMALS; DATABASES, PROTEIN; DRUG DESIGN; GENETIC VECTORS; GENOMICS; HUMANS; MEMBRANE PROTEINS; PROTEIN CONFORMATION; RECEPTORS, G-PROTEIN-COUPLED;

EID: 4043142190     PISSN: 13676733     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (35)

1. Michel H: Membrane proteins of known structure. Max Plank Institute of Biophysics, Frankfurt, Germany. http://www.mpibp-frankfurt.mpg.de/michel/public/ memprotstruct.html.
2. Abramson J, Smirnova I, Kasho V, Verner G, Kaback HR, Iwata S: Structure and mechanism of the lactose permease of Escherichia coli. Science (2003) 301(5633):610-615.
3. Unwin N: Structure and action of the nicotinic acetylcholine receptor explored by electron microscopy. FEBS Lett (2003) 555(1):91-95.
4. Palczewski K, Kumasaka T, Hori T, Behnke CA, Motoshima H, Fox BA, Le Trong I, Teller DC, Okada T, Stenkamp RE, Yamamoto M et al: Crystal structure of rhodopsin: A G protein-coupled receptor. Science (2000) 289(5480):739-745.
5. Drew D, Fröderberg L, Baars L, de Gier J-W: Assembly and overexpression of membrane proteins in Escherichia coli. Biochim Biophys Acta (2003) 1610(1):3-10.
6. Kunji ER, Slotboom D-J, Poolman B: Lactococcus lactis as host for overproduction of functional membrane proteins. Biochim Biophys Acta (2003) 1610(1):97-108.
7. Turner GJ, Reusch R, Winter-Vann AM, Martinez L, Betlach MC: Heterologous gene expression in a membrane-protein-specific system. Protein Expr Purif (1999) 17(2):312-323.
8. Weiss HM, Grisshammer R: Purification and characterization of the human adenosine A2a receptor functionally expressed in Escherichia coli. Eur J Biochem (2002) 269(1):82-92. Successful purification and characterization of the human adenosine A2a receptor overexpressed in E coli membranes.
9. Luca S, White JF, Sohal AK, Filippov DV, van Boom JH, Grisshammer R, Baldus M: The conformation of neurotensin bound to its G protein-coupled receptor. Proc Natl Acad Sci USA (2003) 100(19):10706-10711.
10. Lopez de Maturana R, Willshaw A, Kuntzsch A, Rudolph R, Donelly D: The isolated N-terminal domain of the glucagon-like peptide-1 (GLP-1) receptor binds exendin peptides with much higher affinity than GLP-1. J Biol Chem (2003) 278(12):10195-10200. A description of the successful refolding of the active N-terminal domain of a mammalian GPCR expressed in E coli inclusion bodies.
11. David NE, Gee M, Andersen B, Naider F, Thomer J, Stevens RC: Expression and purification of the Saccharomyces cerevisiae - Factor receptor (Ste2p), a 7-transmembrane-segment G protein-coupled receptor. J Biol Chem (1997) 272(24):15553-15561.
12. 1A dopamine receptor: Heterologous expression in Saccharomyces cerevisiae and purification of the functional receptor. Protein Expr Purif (1998) 13(1):111-119.
13. 2S dopamine receptor in the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe: A comparative study. FEBS Lett (1994) 344(1):41-46.
14. 2-adrenergic receptor produced in the methylotrophic yeast Pichia pastoris. Biochem J (1998) 330(Pt 3):1137-1147. A description of the high-level expression of two human GPCRs in the methylotrophic yeast P pastoris.
15. Mazina KE, Strader CD, Fong TM: Expression and solubilization of a recombinant human neurokinin-1 receptor in insect cells. J Recept Res (1994) 14(1):63-73.
16. Guan XM, Kobilka TS, Kobilka BK: Enhancement of membrane insertion and function in a type IIIb membrane protein following introduction of a cleavable signal peptide. J Biol Chem (1992) 267(31):21995-21998.
17. Hermans E: Generation of model cell lines expressing recombinant G protein-coupled receptors. Methods Mol Biol (2004) 259(2):137-154.
18. Tate CG, Haase J, Baker C, Boorsma M, Magnani F, Vallis Y, Williams DC: Comparison of seven different heterologous protein expression systems for the production of the serotonin transporter. Biochim Biophys Acta (2003) 1610(1):141-153. An excellent comparison of several heterologous expression systems for a recombinant integral membrane protein.
19. Boorsma M, Nieba L, Koller D, Bachmann MF, Bailey JE, Renner WA: A temperature-regulated replicon-based DNA expression system. Nat Biotechnol (2000) 18(4):429-432.
20. Lundstrom K: Semliki Forest virus vectors for rapid high-level expression of integral membrane proteins. Biochim Biophys Acta (2003) 1610(1):90-96. A good description of the application of Semliki Forest virus vectors for the overexpression of integral membrane proteins.
21. Spirin AS, Baranov VI, Ryabova LA, Ovodov SY, Alakhov YB: A continuous cell-free translation system capable of producing polypeptides in high yield. Science (1988) 242(4882):1162-1164.
22. Michel H: Three-dimensional crystals of a membrane protein complex. The photosynthetic reaction centre from Rhodopseudomonas viridis. J Mol Biol (1982) 158(3):567-572.
23. Ostermeier C, Iwata S, Ludwig B, Michel H: Fv fragment-mediated crystallization of the membrane protein bacterial cytochrome c oxidase. Nat Struct Biol (1995) 2(10):842-846.
24. Landau EM, Rosenbusch JP: Lipidic cubic phases: A novel concept for the crystallization of membrane proteins. Proc Natl Acad Sci USA (1996) 93(25):14532-14535.
25. Kyogoku Y, Fujiyoshi Y, Shimada I, Nakamura H, Tsukihara T, Akutsu H, Odahara T, Okada T, Nomura N: Structural genomics of membrane proteins. Acc Chem Res (2003) 36(3):199-206. A review of structural genomics projects.
26. Sato C, Ueno Y, Asai K, Takahashi K, Sato M, Engel A, Fujiyoshi Y: The voltage-sensitive sodium channel is a bell-shaped molecule with several cavities. Nature (2001) 409(6823):1047-1051.
27. Fujiyoshi Y: The structural study of membrane proteins by electron crystallography. Adv Biophys (1998) 35:25-80.
28. Arora A, Abildgaard F, Bushweller JH, Tamm LK: Structure of outer membrane protein A transmembrane domain by NMR spectroscopy. Nat Struct Biol (2001) 8(4):334-338.
29. Nishida N, Sumikawa H, Sakakura M, Shimba N, Takahashi H, Terasawa H, Suzuki EI, Shimada I: Collagen-binding mode of vWF-A3 domain determined by a transferred cross-saturation experiment. Nat Struct Biol (2003) 10(1):53-58.
30. Goulding CW, Apostol M, Anderson DH, Gill HS, Smith CV, Kuo MR, Yang JK, Waldo GS, Sun SW, Chauhan R, Kale A et al: The TB structural genomics consortium: Providing a structural foundation for drug discovery. Curr Drug Targets Infect Disord (2002) 2(2):121-141.
31. Yokoyama S, Hirota H, Kigawa T, Yabuki T, Shirouzu M, Terada T, Ito Y, Matsuo Y, Kuroda Y, Nishimura Y, Kyogoku Y et al: Structural genomics projects in Japan. Nat Struct Biol (2000) 7(Suppl):943-945.
32. Madin K, Sawasaki T, Ogasawara T, Endo Y: A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: Plants apparently contain a suicide system directed at ribosomes. Proc Natl Acad Sci USA (2000) 97(2)559-564.
33. Doi T, Hiroaki Y, Arimono I, Fujiyoshi Y, Okamoto T, Satoh M, Furuichi Y: Characterization of human endothelin B receptor and mutant receptors expressed in insect cells. Eur J Biochem (1997) 248(1):139-148.
34. Adams MW, Dailey HA, DeLucas LJ, Luo M, Prestegard JH, Rose JP, Wang B-C: The Southeast Collaboratory for Structural Genomics: A high-throughput gene to structure factory. Acc Chem Res (2003) 36(3):191-198.
35. Robb FT, Maeder DL, Brown JR, DiRuggiero J, Stump MD, Yeh RK, Weiss RB, Dunn DM: Genomic sequence of hyperthermophile, Pyrococcus furiosus: Implications for physiology and enzymology. Methods Enzymol (2001) 330:134-157.