Small, basic antifungal proteins secreted from filamentous ascomycetes: A comparative study regarding, expression, structure, function and potential application

Applied Microbiology and Biotechnology

Volumn 65, Issue 2, 2004, Pages 133-142

  Marx, F ^a  

^a INNSBRUCK MEDICAL UNIVERSITY   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIFUNGAL AGENT; CYSTEINE; FUNGAL PROTEIN; PROTEIN AFP; PROTEIN ANAFP; PROTEIN GAMA; PROTEIN LFAFP; PROTEIN NAF; PROTEIN PAF; UNCLASSIFIED DRUG;

ANTIMICROBIAL ACTIVITY;

ALKALINITY; AMINO ACID SEQUENCE; ANTIFUNGAL ACTIVITY; ASCOMYCETES; BACTERIUM; CLASSIFICATION; COMPARATIVE STUDY; DRUG BINDING SITE; DRUG MECHANISM; DRUG TARGETING; FUNGAL GENE; FUNGUS; MOLECULAR SIZE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN DNA BINDING; PROTEIN FUNCTION; PROTEIN SECRETION; PROTEIN STRUCTURE; SHORT SURVEY; SPECIES DIFFERENCE; TRANSCRIPTION REGULATION; YEAST;

ASCOMYCOTA; EUKARYOTA; FUNGI; INVERTEBRATA; PROKARYOTA; VERTEBRATA;

EID: 4043071183     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-004-1600-z     Document Type: Short Survey

References (120)

1. Abad LR, D'Urzo MP, Narasimhan ML, Reuveni M, Zhu JK, Niu X, Singh NK, Haegawa PM, Bressan RA (1996) Antifungal activity of tobacco osmotin has specificity and involves plasma membrane permeabilization. Plant Sci 118:11-23
2. Abrami L, Liu S, Cosson P, Leppla SH, Goot FG van der (2003) Anthrax toxin triggers endocytosis of its receptor via a lipid raft-mediated clathrin-dependent process. J Cell Biol 160:321-328
3. Ahmed A, Sesti F, Ilan N, Shih TM, Sturley SL, Goldstein SA (1999) A molecular target for viral killer toxin: TOK1 potassium channel. Cell 99:283-291
4. Baker B, Zambryski P, Staskawicz B, Dinesh-Kumar SP (1997) Signaling in plant-microbe interactions. Science 276:726-733
5. Ballance DJ (1986) Sequences important for gene expression in filamentous fungi. Yeast 2:229-236
6. Bormann C, Baier D, Horr I, Raps C, Berger J, Jung G, Schwarz H (1999) Characterization of a novel, antifungal, chitin-binding protein from Streptomyces tendae Tu901 that interferes with growth polarity. J Bacteriol 181:7421-7429
7. Brackhage AA, Adrianopoulos A, Kato M, Steidl S, Davis MA, Tsukagoshi N, Hynes MJ (1999) HAP-like CCAAT-binding complexes in filamentous fungi: implications for biotechnology. Fungal Genet Biol 27:243-252
8. Bradley KA, Young JAT (2003) Anthrax toxin receptor proteins. Biochem Pharmacol 65:309-314
9. Breinig F, Tipper DJ, Schmitt MJ (2002) Kre1p, the plasma membrane receptor for the yeast K1 viral toxin. Cell 108:395-405
10. Broekaert WF, Terras FR, Cammue BP, Osborn RW (1995) Plant defensins: novel antimicrobial peptides as components of the host defense system. Plant Physiol 108:1353-1358
11. Campos-Olivas R, Bruix M, Santoro J, Lacadena J, Matrinez del Pozo A, Gavilanes JG, Rico M (1995) NMR solution structure of the antifungal protein from Aspergillus giganteus: evidence for cysteine pairing isomerism. Biochemistry 34:3009-3021
12. Cociancich S, Ghazi A, Hetru C, Hoffmann JA, Letellier L (1993) Insect defensin, an inducible antibacterial peptide, forms voltage-dependent channels in Micrococcus luteus. J Biol Chem 268:19239-19245
13. Cubero B, Scazzocchio C (1994) Two different, adjacent and divergent zinc finger binding sites are necessary for CREA-mediated carbon catabolite repression in the proline gene cluster of Aspergillus nidulans. EMBO J 13:407-415
14. Denning DW (1991) Epidemiology and pathogenesis of systemic fungal infections in the immunocompromised host. J Antimicrob Chemother 28:1-16
15. De la Pena P, Barros F, Gascon S, Lazo PS, SR (1981) Effect of yeast killer toxin on sensitive cells of Saccharomyces cerevisiae. J Biol Chem 256:10420-10425
16. De Samblanx GW, Fernandez del Carmen A, Sijtsma L, Schaaper WM, Posthuma GA, Fant F, Meloen RH, Broekaert WF, Amerongen A van (1997) Mutational analysis of a plant defensin from radish (Raphanus sativus L.) reveals two adjacent sites important for antifungal activity. J Biol Chem 272:1171-1179
17. Dimarcq JL, Bulet P, Hetru C, Hoffmann J (1998) Cysteine-rich antimicrobial peptides in invertebrates. Biopolymers 47:465-477
18. Dupont B, Crewe Brown HH, Westermann K, Martins MD, Rex JH, Lortholary J, Kauffmann CA (2000) Mycoses in AIDS. Med Mycol 38:259-267
19. Eisfeld K, Riffer F, Mentges J, Schmitt MJ (2002) Endocytotic uptake and retrograde transport of a virally encoded killer toxin in yeast. Mol Microbiol 37:926-940
20. Endo Y, Oka T, Yokota S, Tsurugi K, Natori Y (1993) The biosynthesis of a cytotoxic protein, alpha-sarcin, in a mold of Aspergillus giganteus. II. Maturation of precursor form of alpha-sarcin in vivo. Tokushima J Exp Med 40:7-12
21. Epand RM, Vogel JH (1999) Diversity of antimicrobial peptides and their mechanisms of action. Biochim Biophys Acta 1462:11-28
22. Epand RF, Umezawa N, Porter EA, Gellman SH, Epand RM (2003) Interactions of the antimicrobial beta-peptide beta-17 with phospholipid vesicles differ from membrane interactions of magainins. Eur J Biochem 270:1240-1248
23. Falnes PO, Sandvig K (2000) Penetration of toxins into cells. Curr Opin Cell Biol 12:407-413
24. Fernandez-Luna JL, Lopez-Otin C, Soriano F, Mendez E (1985) Complete amino acid sequence of the Aspergillus cytotoxin mitogillin. Biochemistry 24:861-867
25. Fischer-Parton S, Parton RM, Hickey PC, Dijksterhuis J, Atkinson HA, Read ND (2000) Confocal microscopy of FM4-64 as a tool for analysing endocytosis and vesicle trafficking in living fungal hyphae. J Microsc 198:246-259
26. Fritig B, Heitz T, Legrand M (1998) Antimicrobial proteins in induced plant defense. Curr Opin Immunol 10:16-22
27. Ganz T (1994) Biosynthesis of defensins and other antimicrobial peptides. Ciba Found Symp 186:62-71
28. Garcia-Olmedo F, Molina A, Alamillo JM, Rodriguez-Palenzuela P (1998) Plant defense peptides. Biopolymers 47:479-491
29. Gasset M, Mancheno JM, Lacadena J, Turnay J, Olmo N, Lizarbe MA, Martinez del Pozo A, Onaderra M, Gavilanes JG (1994) Alpha-sarcin, a ribosome-inactivating protein that translocates across the membrane of phospholipid vesicles. Curr Top Pept Protein Res 1:99-104
30. Geisen R (2000) P. nalgiovense carries a gene which is homologous to the paf gene of P. chrysogenum which codes for an antifungal peptide. Int J Food Microbiol 62:95-101
31. Graessle S, Haas H, Friedlin E, Kürnsteiner H, Stöffler G, Redl B (1997) Regulated system for heterologous gene expression in Penicillium chrysogenum. Appl Environ Microbiol 63:753-756
32. Gupte M, Kulkarni P, Ganguli BN (2002) Antifungal antibiotics. Appl Microbiol Biotechnol 58:46-57
33. Hao JJ, Ye JQ, Yang Q, Gong ZZ, Liu WY, Wang ED (2000) A silent antifungal protein (AFP)-like gene lacking two introns in the mould Trichoderma viride. Biochim Biophys Acta 1475:119-124
34. Hawksworth DL (2001) The magnitude of fungal diversity: the 1.5 million species estimate revisited. Mycol Res 12:1422-1432
35. Hejgaard J, Jacobsen S, Svendsen I (1991) Two antifungal thaumatin-like proteins from barley grain. FEBS Lett 291:127-131
36. Helmerhorst EJ, Breeuwer P, Hof W van't, Walgreen-Wterings E, Oomen LCJM, Veerman ECI, Amerongen AVN, Abee T (1999) The cellular target of histatin 5 on Candida albicans is the energized mitochondrion. J Biol Chem 274:7286-7291
37. Hibberd PL, Rubin RH (1994) Clinical aspects of fungal infection in organ transplant recipients. Clin Infect Dis 19:S33-S40
38. Hoover DM, Wu Z, Tucker K, Lu W, Lubkowski J (2003) Antimicrobial characterization of human beta-defensin 3 derivatives. Antimicrob Agents Chemother 47:2804-2809
39. Hughes P, Dennis E, Whitecross M, Llewellyn D, Gage P (2000) The cytotoxic plant protein, beta-purothionin, forms ion channels in lipid membranes. J Biol Chem 275:823-827
40. Inouye M (1991) Intramolecular chaperone: the role of the propeptide in protein folding. Enzymes 45:314-321
41. Jacobs MJ (1995) The regulation of expression of a gene encoding an antifungal protein of Aspergillus giganteus and its heterologous expression in yeast and plant. PhD thesis, Berlin University of Technology, Berlin
42. Jalving R, Vondervoort PJ van de, Visser J, Schaap PJ (2000) Characterization of the kexin-like maturase of Aspergillus niger. Appl Environ Microbiol 66:363-368
43. Kaiserer L, Oberparleiter C, Weiler-Goerz R, Burgstaller W, Leiter E, Marx F (2003) Characterization of the Penicillium chrysogenum antifungal protein PAF. Arch Microbiol 180:204-210
44. Kamysz W, Okroj M, Lukasiak J (2003) Novel properties of antimicrobial peptides. Acta Biochim Pol 50:461-469
45. Kinal H, Park CM, Berry JO, Koltin Y, Bruenn JA (1995) Processing and secretion of a virally encoded antifungal toxin in transgenic tobacco plants: evidence for a Kex2p pathway in plants. Plant Cell 7:677-688
46. Kobayashi N, McEntee K (1993) Identification of cis and trans components of a novel heat-shock stress regulatory pathway in Saccharomyces cerevisiae. Mol Cell Biol 13:248-256
47. Kozlov YV, Kabishev AA, Fedchenko VI (1987) Cloning and sequencing of Shiga toxin structural genes. Proc Natl Acad Sci USSR 295:740-744
48. Kulmburg P, Mathieu M, Dowzer D, Kelly J, Felenbok B (1993) Specific binding sites in the alcR and alcA promoters of the ethanol regulon for the CREA repressor mediating carbon catabolite repression in Aspergillus nidulans. Mol Microbiol 7:847-857
49. Lacadena J, Martinez del Pozo A, Gasset M, Patino B, Campos-Olivas R, Vazquez C, Martinez-Ruiz A, Mancheno JM, Onaderra M, Gavilanes JG (1995) Characterization of the antifungal protein secreted by the mould Aspergillus giganteus. Arch Biochem Biophys 324:273-281
50. Lamb FI, Roberts LM, Lord JM (1985) Nucleotide sequence of cloned cRNA coding for preproricin. Eur J Biochem 148:265-270
51. Lamy B, Davies J (1991) Isolation and nucleotide sequence of the Aspergillus restrictus gene coding for the ribonucleolytic toxin restrictocin and its expression in Aspergillus nidulans: the leader sequence protects producing strains from suicide. Nucleic Acids Res 19:1001-1006
52. Lamy B, Davies J, Schindler D (1992) The Aspergillus ribonucleolytic toxins (ribotoxins). Targeted Diagn Ther 7:237-258
53. Lee GD, Shin SY, Maeng CY, Jin ZZ, Kim KL, Hahm KS (1999) Isolation and characterization of a novel antifungal peptide from Aspergillus niger. Biochem Biophys Res Commun 263:646-651
54. Lee DG, Park Y, Kim HN, Kim HK, Kim PI, Choi BH, Hahm KS (2002) Antifungal mechanism of an antimicrobial peptide, HP (2-20), derived from N-terminus of Helicobacter pylori ribosomal protein L1 against Candida albicans. Biochem Biophys Res Commun 291:1006-1013
55. Lehrer R, Ganz T (1999) Antimicrobial peptides in mammalian and insect host defence. Curr Opin Immunol 11:23-27
56. Leppla SH (1982) Anthrax toxin edema factor: a bacterial adenylate cyclase that increases cyclic AMP concentrations of eukaryotic cells. Proc Natl Acad Sci USA 79:3162-3166
57. Levitz SM (1992) Overview of host defenses in fungal infections. Clin Infect Dis 14:S37-S42
58. Marr KA, Patterson T, Denning D (2002) Aspergillosis. Pathogenesis, clinical manifestations, and therapy. Infect Dis Clin North Am 16:875-894
59. Martinac B, Zhu H, Kubalski A, Zhou XL, Culbertson M, Bussey H, Kung C (1990) Yeast K1 Killer toxin forms ion channels in sensitive yeast spheroplasts and in artificial liposomes. Proc Natl Acad Sci USA 87:6228-6232
60. Martinez-Ruiz A, Martinez del Pozo A, Lacadena J, Mancheno JM, Onaderra M, Gavilanes JG (1997) Characterization of a natural larger form of the antifungal protein (AFP) from Aspergillus giganteus. Biochim Biophys Acta 1340:81-87
61. Martinez Del Pozo A, Lacadena V, Mancheno JM, Olmo N, Onaderra M, Gavilanes JG (2002) The antifungal protein AFP of Aspergillus giganteus is an OB fold-containing protein that produces condensation of DNA. J Biol Chem 277:46179-46183
62. Marx F, Haas H, Reindl M, Stoffler G, Lottspeich F, Redl B (1995) Cloning, structural organization and regulation of expression of the Penicillium chrysogenum paf gene encoding an abundantly secreted protein with antifungal activity. Gene 167:167-171
63. Meyer V, Stahl U (2002) New insights in the regulation of the afp gene encoding the antifungal protein of Aspergillus giganteus. Curr Genet 42:36-42
64. Meyer V, Stahl U (2003) The influence of co-cultivation on expression of the antifungal protein in Aspergillus giganteus. J Basic Microbiol 43:68-74
65. Meyer V, Wedde M, Stahl U (2002) Transcriptional regulation of the antifungal protein in Aspergillus giganteus. Mol Genet Genomics 266:747-757
66. Montesano R, Roth J, Robert A, Orci L (1982) Non-coated membrane invaginations are involved in binding internalization of cholera and tetanus toxins. Nature 296:651-653
67. Murphy OM, Gould FK (1999) Prevention of nosocomial infection in solid organ transplantation. J Hosp Infect 42:177-183
68. Nakaya K, Omata K, Okahashi I, Nakamura Y, Kolekenbrock H, Ulbrich N (1990) Amino acid sequence and disulfide bridges of an antifungal protein isolated from Aspergillus giganteus. Eur J Biochem 193:31-38
69. Nissen-Meyer J, Nes IF (1997) Ribosomally synthesized antimicrobial peptides: their function, structure, biogenesis, and mechanism of action. Arch Microbiol 167:67-77
70. Oberparleiter C, Kaiserer L, Haas H, Ladurner P, Andratsch M, Marx F (2003) Active internalization of the Penicillium chrysogenum antifungal protein PAF in sensitive aspergilli. Antimicrob Agents Chemother 47:3598-3601
71. Oka T, Natori Y, Tanaka S, Tsurugi K, Endo Y (1990) Complete nucleotide sequence of cDNA for the cytotoxin alpha sarcin. Nucleic Acids Res 18:1897
72. Oldach KH, Becker D, Lorz H (2001) Heterologous expression of genes mediating enhanced fungal resistance in transgenic wheat. Mol Plant Microbe Interact 14:832-838
73. Olmo N, Trunay J, Gonzales de Buitrago G, Lopez de Silanes I, JG G, Lizarbe MA (2001) Cytotoxic mechanism of the ribotoxin α-sarcin. Eur J Biochem 268:2113-2123
74. Oren Z, Shai Y (1998) Mode of action of linear amphipathic alpha-helical antimicrobial peptides. Biopolymers 47:451-463
75. Osborn RW, De Samblanx GW, Thevissen K, Goderis I, Torrekens S, Van Leuven F, Attenborough S, Rees SB, Broekaert WF (1995) Isolation and characterisation of plant defensins from seeds of Asteraceae, Fabaceae, Hippocastanaceae and Saxifragaceae. FEBS Lett 368:257-262
76. Osusky M, Zhou G, Osuska L, Hancock RE, Kay WW, Misra S (2000) Transgenic plants expressing cationic peptide chimeras exhibit broad-spectrum resistance to phytopathogens. Nat Biotechnol 18:1162-1166
77. Papagianni M (2003) Ribosomally synthesized peptides with antimicrobial properties: biosynthesis, structure, function, and applications. Biotechnol Adv 21:465-499
78. Park CM, Berry JO, Bruenn JA (1996) High-level secretion of a virally encoded anti-fungal toxin in transgenic tobacco plants. Plant Mol Biol 30:359-366
79. Penninckx IA, Eggermont K, Terras FR, Thomma BP, De Samblanx GW, Buchala A, Metraux JP, Manners JM, Broekaert WF (1996) Pathogen-induced systemic activation of a plant defensin gene in Arabidopsis follows a salicylic acid-independent pathway. Plant Cell 8:2309-2323
80. Penninckx IA, Thomma BP, Buchala A, Metraux JP, Broekaert WF (1998) Concomitant activation of jasmonate and ethylene response pathways is required for induction of a plant defensin gene in Arabidopsis. Plant Cell 10:2103-2113
81. Pocsi I, Sami L, Leiter E, Majoros L, Szabo B, Emri T, Pusztahelyi T (2001) Searching for new-type antifungal drugs. Acta Microbiol Immunol Hung 48:533-543
82. Punt PJ, Drint-Kuijvenhoven A, Lokman BC, Spencer JA, Jeenes D, Archer DA, Hondel CAMJJ van den (2003) The role of the Aspergillus niger farin-type protease gene in processing of fungal proproteins and fusion proteins. Evidence for alternative processing of recombinant (fasion-)proteins. J Biotechnol 106:23-32
83. Raj PA, Dentino AR (2002) Current status of defensins and their role in innate and adaptive immunity. FEMS Microbiol Lett 206:9-18
84. Riffer F, Eisfeld K, Breinig F, Schmitt MJ (2002) Mutational analysis of K28 preprotoxin processing in the yeast Saccharomyces cerevisiae. Microbiology 148:1317-1328
85. Sandvig K, Grimmer S, Lauvrak SU, Torgersen ML, Skretting G, Deurs B van, Iversen TG (2002) Pathways followed by ricin and Shiga toxin into cells. Histochem Cell Biol 117:131-141
86. Scazzocchio C (2000) The fungal GATA factors. Curr Opin Microbiol 3:126-131
87. Schaaper WM, Posthuma GA, Plaman HH, Sijtsma L, Fant F, Borremans F, Thevissen K, Broekaert W, Meloen RH, Amerongen A van (2001) Synthetic peptides derived from the beta2-beta3 loop of Raphanus sativus antifungal protein 2 that mimic the active site. J Pept Res 57:409-418
88. Schindler DG, Davies JE (1977) Specific cleavage of ribosomal RNA caused by alpha-sarcin. Nucleic Acids Res 4:1097-1110
89. Schmitt MJ, Breinig F (2002) The viral killer system in yeast: from molecular biology to application. FEMS Microbiol Lett 26:257-276
90. Schmitt MJ, Klavehn P, Wang J, Schonig I, Tipper DJ (1996) Cell cycle studies on the mode of action of yeast K28 killer toxin. Microbiology 142:2655-2662
91. Sefton AM (2002) Mechanisms of antimicrobial resistance: their clinical relevance in the new millennium. Drugs 62:557-566
92. Seidah NG, Chretien M (1997) Eukaryotic protein processing: endoproteolysis of precursor proteins. Curr Opin Microbiol 8:602-607
93. Selitrennikoff CP (2001) Antifungal proteins. Appl Environ Microbiol 67:2883-2894
94. Skretting G, Torgersen ML, Deurs B van, Sandvig K (1999) Endocytic mechanisms responsible for uptake of GPI-linked diphtheria toxin receptor. J Cell Sci 112:3899-3909
95. Steinbach WJ, Stevens DA (2003) Review of newer antifungal and immunomodulatory strategies for invasive aspergillosis. Clin Infect Dis 37:S157-S187
96. Strockbine NA, Jackson MP, Sung LM, Holmes RK, O'Brien A (1988) Cloning and sequencing of the genes for Shiga toxin from Shigella dysenteriae type 1. J Bacteriol 170:1116-1122
97. Takahashi S, Ueda M, Tanaka A (2001) Function of the prosequence for in vivo folding and secretion of active Rhizopus oryzae lipase in Saccharomyces cerevisiae. Appl Microbiol Biotechnol 55:454-462
98. Tao J, Ginsberg I, Banerjee N, Held W, Koltin Y, Bruenn JA (1990) Ustilago maydis KP6 killer toxin: structure, expression in Saccharomyces cerevisiae, and relationship to other cellular toxins. Mol Cell Biol 10:1373-1381
99. Terras FR, Schoofs HM, De Bolle MF, Leuven F van, Rees SB, Vanderleyden J, Cammue BP, Broekaert WF (1992) Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds. J Biol Chem 267:15301-15309
100. Terras FR, Torrekens S, Leuven F van, Osborn RW, Vanderleyden J, Cammue BP, Broekaert WF (1993) A new family of basic cysteine-rich plant antifungal proteins from Brassicaceae species. FEBS Lett 316:233-240
101. Theis T, Stahl U (2004) Antifungal proteins: targets, mechanisms and prospective applications. Cell Mol Life Sci 61:437-455
102. Theis T, Wedde M, Meyer V, Stahl U (2003) The antifungal protein from Aspergillus giganteus causes membrane permeabilization. Antimicrob Agents Chemother 47:588-593
103. Thevissen K, Ghazi A, De Samblanx GW, Brownlee C, Osborn RW, Broekaert WF (1996) Fungal membrane responses induced by plant defensins and thionins. J Biol Chem 271:15018-15025
104. Thevissen K, Osborn RW, Acland DP, Broekaert WF (1997) Specific, high affinity binding sites for an antifungal plant defensin on Neurospora crassa hyphae and microsomal membranes. J Biol Chem 272:32176-32181
105. Thevissen K, Terras FR, Broekaert WF (1999) Permeabilization of fungal membranes by plant defensins inhibits fungal growth. Appl Environ Microbiol 65:5451-5458
106. Thevissen K, Francois IEJA, Takemoto JY, Ferket KK, Meert EMK, Cammue BP (2003) DmAMP1, an antifungal plant defensin from dahlia (Dahlia merckii), interacts with sphingolipids from Saccharomyces cerevisiae. FEMS Microbiol Lett 226:169-173
107. Tilburn J, Sarkar S, Widdick DA, Espeso EA, Orejas M, Mungroo J, Penalva MA, Arst HN (1995) The Aspergillus PacC zinc finger transcription factor mediates regulation of both acid-and alkaline-expressed genes by ambient pH. EMBO J 14:779-790
108. Toone WM, Jones N (1998) Stress-activated signalling pathways in yeast. Genes Cells 3:485-498
109. Torralba S, Heath BI (2002) Analysis of three separate probes suggests the absence of endocytosis in Neurospora crassa hyphae. Fungal Genet Biol 37:221-232
110. Tossi A, Sandri L, Giangaspero A (2000) Amphipathic, alpha-helical antimicrobial peptides. Biopolymers 55:4-30
111. Vanholder R, Van Biesen W (2002) Incidence of infectious morbidity and mortality in dialysis patients. Blood Purif 20:477-480
112. Vila L, Lacadena V, Fontanet P, Martinez del Pozo A, San Segundo B (2001) A protein from the mold Aspergillus giganteus is a potent inhibitor of fungal plant pathogens. Mol Plant Microbe Interact 14:1327-1331
113. Vollebregt AWH, Van Solingen P, Bovernberg RAL (1994) Isolation and characterization of gene Y of Penicillium chrysogenum: close relation of the gene product to the antifungal protein of Aspergillus giganteus. Abstr Eur Conf Fungal Genet 2
114. Walsh TJ, Pizzo A (1988) Treatment of systemic fungal infections: recent progress and current problems. Eur J Clin Microbiol Infect Dis 7:460-475
115. Westerhoff HV, Juretic D, Hendler RW, Zasloff M (1989) Magainins and the disruption of membrane-linked free-energy transduction. Proc Natl Acad Sci USA 86:6597-6601
116. Wnendt S, Felske-Zech H, Henze PPC, Ulbrich N, Stahl U (1993) Characterization of the gene encoding alpha-sarcin, a ribosome-inactivating protein secreted by Aspergillus giganteus. Gene 124:239-244
117. Wnendt S, Ulbrich N, Stahl U (1994) Molecular cloning, sequence analysis and expression of the gene encoding an antifungal-protein from Aspergillus giganteus. Curr Genet 25:519-523
118. Wool IG, Glück A, Endo Y (1992) Ribotoxin recognition of ribosomal RNA and a proposal for the mechanism of translocation. Trends Biochem Sci 17:266-269
119. Xu J, Gong ZZ (2003) Intron requirement for AFP gene expression in Trichoderma viride. Microbiology 149:3093-3097
120. Yamashita RA, May GS (1998) Constitutive activation of endocytosis by mutation of myoA, the myosin I gene of Aspergillus nidulans. J Biol Chem 273:14644-14648