Proliferative response of bystander cells adjacent to cells, with incorporated radioactivity

Cytometry Part A

Volumn 60, Issue 2, 2004, Pages 155-164

  Gerashchenko, Bogdan I ^a   Howell, Roger W ^a  

^a RUTGERS NEW JERSEY MEDICAL SCHOOL   (United States)

Author keywords

Bystander effects; Co culture; Flow cytometry; Proliferation; Radiolabeled cells; Tritiated thymidine; Tritium; Unlabeled cells; particles

Indexed keywords

CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER; METHYLTHYMIDINE H 3; RADIOISOTOPE; THYMIDINE DERIVATIVE; TRITIUM; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BYSTANDER EFFECT; CELL CULTURE; CELL GROWTH; CELL PROLIFERATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ELECTRON; EPITHELIUM CELL; FLOW CYTOMETRY; GAMMA RADIATION; ISOTOPE LABELING; LIVER CELL; NONHUMAN; PRIORITY JOURNAL; RADIATION DOSE; RADIOACTIVITY; RAT; X IRRADIATION;

EID: 4043069119     PISSN: 15524922     EISSN: None     Source Type: Journal    
DOI: 10.1002/cyto.a.20029     Document Type: Article

References (53)

1. NCRP. Tritium and other radionuclide labelled organic compounds incorporated in genetic material. Report No. 63. National Council on Radiation Protection and Measurements; Washington, D.C. 1979. 147 p.
2. Straume T, Carsten AL. Tritium radiobiology and relative biological effectiveness. Health Phys 1993;65:657-672.
3. Painter RB, Drew RM, Hughes WL. Inhibition of HeLa growth by intranuclear tritium. Science 1958;127:1244-1245.
4. Drew RM, Painter RB. Action of tritiated thymidine on the clonal growth of mammalian cells. Radiat Res 1959;11:535-544.
5. Wimber DE. Chromosome breakage produced by tritium-labeled thymidine in Tradescantia paludosa. Proc Natl Acad Sci USA 1959;45:839-846.
6. Hori TA, Nakai S. Unusual dose-response of chromosome aberrations induced in human lymphocytes by very low dose exposures to tritium. Mutat Res 1978;50:101-110.
7. Dewey WC, Sedita BA, Humphrey RM. Chromosomal aberrations induced by tritiated thymidine during the S and G2 phases of Chinese hamster cells. Int J Radiat Biol Relat Stud Phys Chem Med 1967;12:597-600.
8. Cleaver JE, Thomas GH, Burki HJ. Biological damage from intranuclear tritium: DNA strand breaks and their repair. Science 1972;177:996-998.
9. Ehmann UK, Williams JR, Nagle WA, Brown JA, Belli JA, Lett JT. Perturbations in cell cycle progression from radioactive DNA precursors. Nature 1975;258:633-636.
10. Pollack A, Bagwell CB, Irvin GL III. Radiation from tritiated thymidine perturbs the cell cycle progression of stimulated lymphocytes. Science 1979;203:1025-1027.
11. 3H]thymidine into human haemopoietic cell lines. Int J Radiat Biol 2000;76:295-303.
12. Bedford JS, Mitchell JB, Griggs HG, Bender MA. Cell killing by gamma rays and beta particles from tritiated water and incorporated tritiated thymidine. Radiat Res 1975;63:531-543.
13. Howell RW, Goddu SM, Bishayee A, Rao DV. Radioprotection against lethal damage caused by chronic irradiation with radionuclides in vitro. Radiat Res 1998;150:391-399.
14. Lisco H, Baserga R, Kisieleski WE. Induction of tumors in mice with tritiated thymidine, Nature 1961;192:571-572.
15. Bishayee A, Rao DV, Howell RW. Evidence for pronounced bystander effects caused by nonuniform distributions of radioactivity using a novel three-dimensional tissue culture model. Radiat Res 1999;152:88-97.
16. Bishayee A, Hill HZ, Stein D, Rao DV, Howell RW. Free-radical initiated and gap junction-mediated bystander effect due to nonuniform distribution of incorporated radioactivity in a three-dimensional tissue culture model. Radiat Res 2001;155:335-344.
17. Little JB. Radiation carcinogenesis. Carcinogenesis 2000;21:394-404.
18. Mothersill C, Seymour C. Radiation-induced bystander effects: past history and future directions. Radiat Res 2001;155:759-767.
19. Goldberg Z, Lehnert BE. Radiation-induced effects in unirradiated cells: a review and implications in cancer. Int J Oncol 2002;21:337-349.
20. Morgan WF. Non-targeted and delayed effects of exposure to ionizing radiation: I. Radiation-induced genomic instability and bystander effects in vitro. Radiat Res 2003;159:567-580.
21. Morgan WF. Non-targeted and delayed effects of exposure to ionizing radiation: II. Radiation-induced genomic instability and bystander effects in vivo, clastogenic factors and transgenerational effects. Radiat Res 2003;159:581-596.
22. Azzam EI, de Toledo SM, Little JB. Oxidative metabolism, gap junctions and the ionizing radiation-induced bystander effect. Oncogene 2003;22:7050-7057.
23. Iyer R, Lehnert BE. Factors underlying the cell growth-related by-stander responses to alpha particles. Cancer Res 2000;60:1290-1298.
24. Shao C, Furusawa Y, Aoki M, Matsumoto H, Ando K. Nitric oxide-mediated bystander effect induced by heavy-ions in human salivary gland tumour cells. Int J Radiat Biol 2002;78:837-844.
25. Gerashchenko BI, Howell RW. Flow cytometry as a strategy to study radiation-induced bystander effects in co-culture systems. Cytometry 2003;54A:1-7.
26. Gerashchenko BI, Howell RW. Cell proximity is a prerequisite for the proliferative response of bystander cells co-cultured with cells irradiated with gamma-rays. Cytometry 2003;56A:71-80.
27. Tsao MS, Smith JD, Nelson KG, Grisham JW. A diploid epithelial cell line from normal adult rat liver with phenotypic properties of 'oval' cells. Exp Cell Res 1984;154:38-52.
28. Haughland RP. Handbook of fluorescent probes and research chemicals. 6th edition. Eugene, OR: Molecular Probes; 1996. p 377-398.
29. Goddu SM, Howell RW, Bouchet LG, Bolch WE, Rao DV. MIRD Cellular S values: self-absorbed dose per unit cumulated activity for selected radionuclides and monoenergetic electron and alpha particle emitters incorporated into different cell compartments. Reston, VA: Society of Nuclear Medicine; 1997. 183 p.
30. Howell RW, Rao DV, Hou D-Y, Narra VR, Sastry KSR. The question of relative biological effectiveness and quality factor for Auger emitters incorporated into proliferating mammalian cells. Radiat Res 1991; 128:282-292.
31. Browne E, Firestone RB. Table of radioactive isotopes. New York: Wiley; 1986.
32. Nagasawa H, Kassis AI, Berman RM, Sahu SK, Nickoloff JA, Okinaka RT, Adelstein SJ, Little JB. Comparison of mutation induction by external and internal radiation sources in synchronized Chinese hamster ovary (CHO) cells. In: Howell RW, Narra VR, Sastry KSR, Rao DV, editors. Biophysical aspects of auger processes. Woodbury, NY: American Institute of Physics; 1992. p 194-209.
33. Marko NF, Dieffenbach PB, Yan G, Ceryak S, Howell RW, McCaffrey TA, Hu VW. Does metabolic radiolabeling stimulate the stress response? Differential cellular responses to internal beta versus external gamma radiation. FASEB J 2003;17:1470-1486.
34. Azzam EI, de Toledo SM, Gooding T, Little JB. Intercellular communication is involved in the bystander regulation of gene expression in human cells exposed to very low fluences of alpha particles. Radiat Res 1998;150:497-504.
35. Azzam EI, de Toledo SM, Little JB. Direct evidence for the participation of gap-junction mediated intercellular communication in the transmission of damage signals from alpha-particle irradiated to non-irradiated cells. Proc Natl Acad Sci USA 2001;98:473-478.
36. Azzam EI, de Toledo SM, Little JB. Expression of CONNEXIN43 is highly sensitive to ionizing radiation and other environmental stresses. Cancer Res 2003;63:7128-7135.
37. Azzam EI, de Toledo SM, Little JB. Stress signaling from irradiated to non-irradiated cells. Curr Canc Drug Targ 2003;4:53-64.
38. Nagasawa H, Little JB. Induction of sister chromatid exchanges by extremely low doses of alpha-particles. Cancer Res 1992;52:6394-6396.
39. Nagasawa H, Little JB. Unexpected sensitivity to the induction of mutations by very low doses of alpha-particle irradiation: Evidence for a bystander effect. Radiat Res 1999;152:552-557.
40. Sigg M, Crompton NEA, Burkhart W. Enhanced neoplastic transformation in an inhomogeneous radiation field: An effect of the presence of heavily damaged cells. Radiat Res 1997;148:543-547.
41. Sawant SG, Randers-Pehrson G, Geard CR, Brenner DJ, Hall EJ. The bystander effect in radiation oncogenesis: I. Transformation in C3H 10T1/2 cells in vitro can be initiated in the unirradiated neighbors of irradiated cells. Radiat Res 2001;155:397-401.
42. 125I. Micron 2002;33:127-132.
43. Zhou H, Randers-Pehrson G, Waldren CA, Vannais D, Hall EJ, Hei TK. Induction of a bystander mutagenic effect of alpha particles in mammalian cells. Proc Natl Acad Sci USA 2000;97:2099-2104.
44. Hickman AW, Jaramillo RJ, Lechner JF, Johnson NF. Alpha-particle-induced p53 protein expression in a rat lung epithelial cell strain. Cancer Res 1994;54:5797-5800.
45. Lehnert BE, Goodwin EH. Extracellular factor(s) following exposure to alpha particles can cause sister chromatid exchanges in normal human cells. Cancer Res 1997;57:2164-2171.
46. Narayanan PK, Goodwin EH, Lehnert BE. Alpha particles initiate biological production of superoxide anions and hydrogen peroxide in human cells. Cancer Res 1997;57:3963-3971.
47. Mothersill C, Seymour CB. Cell-cell contact during gamma irradiation is not required to induce a bystander effect in normal human keratinocytes: evidence for release during irradiation of a signal controlling survival into the medium. Radiat Res 1998;149:252-262.
48. Narayanan PK, LaRue KEA, Goodwin EH, Lehnert BE. Alpha particles induce the production of interleukin-8 by human cells. Radiat Res 1999;152:57-63.
49. 35S-Methionine induces DNA fragmentation and p53-dependent ROS production. FASEB J 2001;15:1562-1568.
50. Burdon RH, Gill V, Rice-Evans C. Cell proliferation and oxidative stress. Free Radic Res Commun 1989;7:149-159.
51. Murrell GA, Francis MJ, Bromley L. Modulation of fibroblast proliferation by oxygen free radicals. Biochem J 1990;265:659-665.
52. Burdon RH, Gill V. Cellularly generated active oxygen species and HeLa cell proliferation. Free Radic Res Commun 1993;19:203-213.
53. Azzam El, de Toledo SM, Spitz DR, Little JB. Oxidative metabolism modulates signal transduction and micronucleus formation in by-stander cells from alpha-particle-irradiated normal human fibroblast cultures. Cancer Res 2002;62:5436-5442.