Flow Cytometry as a Strategy to Study Radiation-Induced Bystander Effects in Co-Culture Systems

Cytometry Part A

Volumn 54, Issue 1, 2003, Pages 1-7

  Gerashchenko, Bogdan I ^a   Howell, Roger W ^a  

^a RUTGERS NEW JERSEY MEDICAL SCHOOL   (United States)

Author keywords

Bystander effects; Co culture; DiI; Flow cytometry; Gamma radiation; Proliferation

Indexed keywords

CESIUM 137; FLUORESCENT DYE;

ANIMAL CELL; ARTICLE; BYSTANDER EFFECT; CELL GROWTH; CELL LINE; CELL POPULATION; CELL PROLIFERATION; COCULTURE; CONTROLLED STUDY; EPITHELIUM CELL; FLOW CYTOMETRY; GAMMA RADIATION; IONIZING RADIATION; MONOLAYER CULTURE; NONHUMAN; PRIORITY JOURNAL; RADIATION DOSE; RADIATION RESPONSE; RAT;

EID: 0347386413     PISSN: 15524922     EISSN: None     Source Type: Journal    
DOI: 10.1002/cyto.a.10049     Document Type: Article

References (36)

1. Nagasawa H, Little JB. Induction of sister chromatid exchanges by extremely low doses of alpha-particles. Cancer Res 1992;52:6394-6396.
2. Hickman AW, Jaramillo RJ, Lechner JF, Johnson NF. Alpha-particle-induced p53 protein expression in a rat lung epithelial cell strain. Cancer Res 1994;54:5797-5800.
3. Deshpande A, Goodwin EH, Balley SM, Marrone BL, Lehnert BE. Alpha-particle-induced sister chromatid exchange in normal human lung fibroblasts - evidence for an extranuclear target. Radiat Res 1996;145:260-267.
4. Sigg M, Crompton NEA, Burkhart W. Enhanced neoplastic transformation in an inhomogeneous radiation field: an effect of the presence of heavily damaged cells. Radiat Res 1997;148:543-547.
5. Azzam EI, de Toledo SM, Gooding T, Little JB. Intercellular communication is involved in the bystander regulation of gene expression in human cells exposed to very low fluences of alpha particles. Radiat Res 1998;150:497-504.
6. Prise KM, Belyakov OV, Folkard M, Micheal BD. Studies on bystander effects in human fibroblasts using a charged particle microbeam. Int J Radiat Biol 1998;74:793-798.
7. Lorimore SA, Kadhim MA, Pocock DA, Papworth D, Stevens DL, Goodhead DT, Wright EG. Chromosomal instability in the descendants of unirradiated surviving cells after alpha-particle irradiation. Proc Natl Acad Sci U S A 1998;95:5730-5733.
8. Bishayee A, Rao DV, Howell RW. Evidence for pronounced bystander effects caused by nonuniform distributions of radioactivity using a novel three-dimensional tissue culture model. Radiat Res 1999;152:88-97.
9. Bishayee A, Hill HZ, Stein D, Rao DV, Howell RW. Free-radical initiated and gap junction-mediated bystander effect due to nonuniform distribution of incorporated radioactivity in a three-dimensional tissue culture model. Radiat Res 2001;155:335-344.
10. Zhou H, Randers-Pehrson G, Waldren CA, Vannais D, Hall EJ, Hei TK. Induction of a bystander mutagenic effect of alpha particles in mammalian cells. Proc Natl Acad Sci U S A 2000;97:2099-2104.
11. Belyakov OV, Malcolmson AM, Folkard M, Prise KM, Michael BD. Direct evidence for a bystander effect of ionizing radiation in primary human fibroblasts. Br J Cancer 2001;84:674-679.
12. Sawant SG, Randers-Pehrson G, Geard CR, Brenner DJ, Hall EJ. The bystander effect in radiation oncogenesis: I. Transformation in C3H 10T1/2 cells in vitro can be initiated in the unirradiated neighbors of irradiated cells. Radiat Res 2001;155:397-401.
13. 125I. Micron 2002;33:127-132.
14. Lehnert BE, Goodwin EH. Extracellular factor(s) following exposure to alpha particles can cause sister chromatid exchanges in normal human cells. Cancer Res 1997;57:2164-2171.
15. Mothersill C, Seymour C. Medium from irradiated human epithelial cells but not human fibroblasts reduces the clonogenic survival of unirradiated cells. Int J Radiat Biol 1997;71:421-427.
16. Mothersill C, Seymour CB. Cell-cell contact during gamma irradiation is not required to induce a bystander effect in normal kidney keratinocytes: evidence for release during irradiation of a signal controlling survival into the medium. Radiat Res 1998;149:252-262.
17. Azzam EI, de Toledo SM, Little JB. Direct evidence for the participation of gap-junction mediated intercellular communication in the transmission of damage signals from alpha-particle irradiated to non-irradiated cells. Proc Natl Acad Sci U S A 2001;98:473-478.
18. Narayanan PK, Goodwin EH, Lehnert BE. Alpha particles initiate biological production of superoxide anions and hydrogen peroxide in human cells. Cancer Res 1997;57:3963-3971.
19. Narayanan PK, LaRue KEA, Goodwin EH, Lehnert BE. Alpha particles induce the production of interleukin-8 by human cells. Radiat Res 1999;152:57-63.
20. Iyer R, Lehnert BE. Factors underlying the cell growth-related by-stander responses to alpha particles. Cancer Res 2000;60:1290-1298.
21. Haughland RP. Handbook of fluorescent probes and research chemicals, 6th edition. Eugene, OR: Molecular Probes; 1996. 344-350.
22. Tsao MS, Smith JD, Nelson KG, Grisham JW. A diploid epithelial cell line from normal adult rat liver with phenotypic properties of 'oval' cells. Exp Cell Res 1984;154:38-52.
23. Wilson MR, Close TW, Trosko JE. Cell population dynamics (apoptosis, mitosis, and cell-cell communication) during disruption of homeostasis. Exp Cell Res 2000;254:257-268.
24. Little JB. Radiation carcinogenesis. Carcinogenesis 2000;21:394-404.
25. Hill HZ, Hill GJ, Miller CF, Kwong F, Purdy J. Radiation and melanoma: response of B16 mouse tumor cells and clonal lines to in vitro irradiation. Radiat Res 1979;80:259-276.
26. Wells J, Berry RJ, Laing AH. The effect of irradiated feeder cells on the X-ray survival curve shape of freshly explanted human tumor cells and a standard human tumor cell line. Radiat Res 1980;81:150-156.
27. Rodriguez A, Alpen EL. Feeder cells and cell survival in spheroids and monolayers. Int J Radiat Biol Relat Stud Phys Chem Med 1982;41:111-117.
28. Azzam EI, Raaphorst GP, Mitchel RE. Radiation-induced adaptive response for protection against micronucleus formation and neoplastic transformation in C3H 10T1/2 mouse embryo cells. Radiat Res 1994;138:S28-S31.
29. Azzam EI, de Toledo SM, Raaphorst GP, Mitchel RE. Radiation-induced radioresistance in a normal human skin fibroblast cell line. In: Sugahara T, Sagan LA, Aoyama T, editors. Low dose irradiation and biological defense mechanisms. Amsterdam: Elsevier; 1992. p 291-294.
30. Planel H, Soleilhavoup JP, Tixador R, Richoilley G, Conter A, Croute F, Caratero C, Gaubin Y. Influence on cell proliferation of background radiation or exposure to very low, chronic gamma radiation. Health Phys 1987;52:571-578.
31. Wang GJ, Cai L. Induction of cell-proliferation hormesis and cell-survival adaptive response in mouse hematopoietic cells by whole-body low-dose radiation. Toxicol Sci 2000;53:369-376.
32. Kumar NM, Gilula NB. The gap junction communication channel. Cell 1996;84:381-388.
33. Loewenstein WR, Rose B. The cell-cell channel in the control of growth. Semin Cell Biol 1992;3:59-79.
34. Burdon RH, Gill V. Cellularly generated active oxygen species and HeLa cell proliferation. Free Radic Res Commun 1993;19:203-213.
35. Burdon RH, Gill V, Rice-Evans C. Cell proliferation and oxidative stress. Free Radic Res Commun 1989;7:149-159.
36. Murrell GA, Francis MJ, Bromley L. Modulation of fibroblast proliferation by oxygen free radicals. Biochem J 1990;265:659-665.