Cloning of an intracellular D(-)-3-hydroxybutyrate-oligomer hydrolase gene from Ralstonia eutropha H16 and identification of the active site serine residue by site-directed mutagenesis

Journal of Bioscience and Bioengineering

Volumn 94, Issue 2, 2002, Pages 106-112

  Haruhisa, Saegusa ^a   Mari, Shiraki ^b   Terumi, Saito ^b  

^a KANAGAWA UNIVERSITY   (Japan)

^b Faculty of Science   (Japan)

Author keywords

Intracellular 3 hydroxybutyrate oligomer hydrolase; Ralstonia eutropha; Serine esterase

Indexed keywords

CLONING; DNA; ENZYMES; ESCHERICHIA COLI; MOLECULAR WEIGHT; MUTAGENESIS; OLIGOMERS; POLYPEPTIDES;

SUPERNATANT FRACTION;

GENES;

3 HYDROXYBUTYRATE HYDROLASE; 3 HYDROXYBUTYRIC ACID; DNA; OLIGOMER; PLASMID VECTOR; SERINE PROTEINASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CONSENSUS SEQUENCE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME LOCALIZATION; ESCHERICHIA COLI; HYBRIDIZATION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PROTEIN EXPRESSION; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; WAUTERSIA EUTROPHA;

ALCALIGENES; ESCHERICHIA COLI; NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS SP.; RALSTONIA; RALSTONIA EUTROPHA; RALSTONIA PICKETTII; WAUTERSIA EUTROPHA;

EID: 0036379752     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(02)80128-1     Document Type: Article

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