Identification and characterization of the Bacillus thuringiensis phaZ gene, encoding new intracellular poly-3-hydroxybutyrate depolymerase

Journal of Bacteriology

Volumn 188, Issue 21, 2006, Pages 7592-7599

  Tseng, Chi Ling ^a   Chen, Hui Ju ^a   Shaw, Gwo Chyuan ^a  

^a NATIONAL YANG MING UNIVERSITY   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

3 HYDROXYBUTYRIC ACID; 4 NITROPHENYLPALMITATE; BACTERIAL ENZYME; MONOMER; PALMITIC ACID DERIVATIVE; POLY 3 HYDROXYBUTYRATE DEPOLYMERASE; POLY(3 HYDROXYBUTYRIC ACID); PROTEIN PHAZ; SIGNAL PEPTIDE; TRIBUTYRIN; TRIOLEIN; TRYPSIN; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS THURINGIENSIS; BACTERIAL GENE; BACTERIAL GENOME; BACTERIAL GROWTH; BACTERIUM CULTURE; CULTURE MEDIUM; GENE IDENTIFICATION; GENE MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHAZ GENE; PRIORITY JOURNAL; PSEUDOMONAS PUTIDA; SUPERNATANT; ULTRASOUND; UNINDEXED SEQUENCE; WILD TYPE;

BACILLUS THURINGIENSIS; BASE SEQUENCE; BINDING SITES; CARBOXYLIC ESTER HYDROLASES; COMPLEX MIXTURES; GENE DELETION; HYDROXYBUTYRATES; MOLECULAR SEQUENCE DATA; PALMITATES; POLYESTERS; PROMOTER REGIONS (GENETICS); PROTEIN SORTING SIGNALS; SUBSTRATE SPECIFICITY; TRANSCRIPTION INITIATION SITE; TRIGLYCERIDES; TRIOLEIN;

BACILLUS THURINGIENSIS; BACILLUS THURINGIENSIS SEROVAR ISRAELENSIS; PSEUDOMONAS PUTIDA;

EID: 33750464109     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.00729-06     Document Type: Article

References (40)

1. Abe, T., T. Kobayashi, and T. Saito. 2005. Properties of a novel intracellular poly(3-hydroxybutyrate) depolymerase with high specific activity (PhaZd) in Wautersia eutropha H16. J. Bacteriol. 187:6982-6990.
2. Bone, E. J., and D. J. Ellar. 1989. Transformation of Bacillus thuringiensis by electroporation. FEMS Microbiol. Lett. 58:171-177.
3. Brandl, H., R. A. Gross, R. W. Lenz, and R. C. Fuller. 1988. Pseudomonas oleovorans as source of poly-3-hydroxyalkanoates for potential applications as biodegradable polyesters. Appl. Environ. Microbiol. 54:1977-1982.
4. Chen, G. Q., K. H. Konig, and R. M. Lafferty. 1991. Occurrence of poly-D(-)-3-hydroxyalkanoates in the genus Bacillus. FEMS Microbiol. Lett. 68:173-176.
5. Chiou, C. Y., H. H. Wang, and G. C. Shaw. 2002. Identification and characterization of the non-PTS fru locus of Bacillus megaterium ATCC 14581. Mol. Genet. Genomics 268:240-248.
6. Delafield, F. P., M. Doudoroff, N. J. Palleroni, C. J. Lusty, and R. Contopoulos. 1965. Decomposition of poly-beta-hydroxybutyrate by pseudomonads. J. Bacteriol. 90:1455-1466.
7. Fedhila, S., T. Msadek, P. Nel, and D. Lereclus. 2002. Distinct clpP genes control specific adaptive responses in Bacillus thuringiensis. J. Bacteriol. 184:5554-5562.
8. Gao, D., A. Maehara, T. Yamane, and S. Ueda. 2001. Identification of the intracellular polyhydroxyalkanoate depolymerase gene of Paracoccus denitrificans and some properties of the gene product. FEMS Microbiol. Lett. 196:159-164.
9. Handrick, R., S. Reinhardt, M. L. Focarete, M. Scandola, G. Adamus, M. Kowalczuk, and D. Jendrossek. 2001. A new type of thermoalkalophilic hydrolase of Paucimonas lemoignei with high specificity for amorphous polyesters of short chain-length hydroxyalkanoic acids. J. Biol. Chem. 276:36215-36224.
10. Handrick, R., S. Reinhardt, P. Kimmig, and D. Jendrossek. 2004. The "intracellular" poly(3-hydroxybutyrate) (PHB) depolymerase of Rhodospirillum rubrum is a periplasm-located protein with specificity for native PHB and with structural similarity to extracellular PHB depolymerases. J. Bacteriol. 186:7243-7253.
11. Handrick, R., S. Reinhardt, D. Schultheiss, T. Reichart, D. Schuler, V. Jendrossek, and D. Jendrossek. 2004. Unraveling the function of the Rhodospirillum rubrum activator of polyhydroxybutyrate (PHB) degradation: the activator is a PHB-granule-bound protein (phasin). J. Bacteriol. 186:2466-2475.
12. Hartnett, G. B., and L. N. Ornston. 1994. Acquisition of apparent DNA slippage structures during extensive evolutionary divergence of pcaD and catD genes encoding identical catalytic activities in Acinetobacter calcoaceticus. Gene 142:23-29.
13. He, J., S. Chen, and Z. Yu. 2002. Determination of poly-beta- hydroxybutyric acid in Bacillus thuringiensis by capillary zone electrophoresis with indirect ultraviolet absorbance detection. J. Chromatogr. A 973:197-202.
14. Higuchi, R., B. Krummel, and R. K. Saiki. 1988. A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions. Nucleic Acids Res. 16:7351-7367.
15. Jaeger, K. E., S. Ransac, B. W. Dijkstra, C. Colson, M. van Heuvel, and O. Misset. 1994. Bacterial lipases. FEMS Microbiol. Rev. 15:29-63.
16. Jendrossek, D. 2002. Extracellular polyhydroxyalkanoate depolymerases: the key enzymes of PHA degradation, p. 41-83. In Y. Doi and A. Steinbuchel (ed.), Biopolymers, vol. 3b: polyesters II. Wiley-VCH, Weinheim, Germany.
17. Jendrossek, D., and R. Handrick. 2002. Microbial degradation of polyhydroxyalkanoates. Annu. Rev. Microbiol. 56:403-432.
18. Kadouri, D., E. Jurkevitch, and Y. Okon. 2003. Poly-beta-hydroxybutyrate depolymerase (PhaZ) in Azospirillum brasilense and characterization of a phaZ mutant. Arch. Microbiol. 180:309-318.
19. Kobayashi, T., K. Nishikori, and T. Saito. 2004. Properties of an intracellular poly(3-hydroxybutyrate) depolymerase (PhaZ1) from Rhodobacter sphaeroides. Curr. Microbiol. 49:199-202.
20. Kobayashi, T., M. Shiraki, T. Abe, A. Sugiyama, and T. Saito. 2003. Purification and properties of an intracellular 3-hydroxybutyrate-oligomer hydrolase (PhaZ2) in Ralstonia eutropha H16 and its identification as a novel intracellular poly(3-hydroxybutyrate) depolymerase. J. Bacteriol. 185:3485-3490.
21. Kobayashi, T., K. Uchino, T. Abe, Y. Yamazaki, and T. Saito. 2005. Novel intracellular 3-hydroxybutyrate-oligomer hydrolase in Wautersia eutropha H16. J. Bacteriol. 187:5129-5135.
22. Lee, T. R., J. S. Lin, S. S. Wang, and G. C. Shaw. 2004. PhaQ, a new class of poly-beta-hydroxybutyrate (PHB)-responsive repressor, regulates phaQ and phaP (phasin) expression in Bacillus megaterium through interaction with PHB. J. Bacteriol. 186:3015-3021.
23. Lorite, M. J., J. Sanjuan, L. Velasco, J. Olivares, and E. J. Bedmar. 1998. Characterization of Bradyrhizobium japonicum peaBDC genes involved in 4-hydroxybenzoate degradation. Biochim. Biophys. Acta 1397:257-261.
24. McCool, G. J., and M. C. Cannon. 1999. Polyhydroxyalkanoate inclusion body-associated proteins and coding region in Bacillus megaterium. J. Bacteriol. 181:585-592.
25. McCool, G. J., and M. C. Cannon. 2001. PhaC and PhaR are required for polyhydroxyalkanoic acid synthase activity in Bacillus megaterium. J. Bacteriol. 183:4235-4243.
26. McCool, G. J., T. Fernandez, N. Li, and M. C. Cannon. 1996. Polyhydroxyalkanoate inclusion-body growth and proliferation in Bacillus megaterium. FEMS Microbiol. Lett. 138:41-48.
27. Merrick, J. M., R. Steger, and D. Dombroski. 1999. Hydrolysis of native poly(hydroxybutyrate) granules (PHB), crystalline PHB, and artificial amorphous PHB granules by intracellular and extracellular depolymerases. Int. J. Biol. Macromol. 25:129-134.
28. Nelson, K. E., C. Weinel, I. T. Paulsen, R. J. Dodson, H. Hubert, et al. 2002. Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440. Environ. Microbiol. 4:799-808.
29. Nielsen, H., J. Engelbrecht, S. Brunak, and G. von Heijne. 1997. A neural network method for identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Int. J. Neural Syst. 8:581-599.
30. Pötter, M., H. Müller, F. Reinecke, R. Wieczorek, F. Fricke, B. Bowien, B. Friedrich, and A. Steinbüchel. 2004. The complex structure of polyhydroxybutyrate (PHB) granules: four orthologous and paralogous phasins occur in Ralstonia eutropha. Microbiology 150:2301-2311.
31. Ray, C., R. E. Hay, H. L. Carter, and C. P. Moran. 1985. Mutations that affect utilization of a promoter in stationary-phase Bacillus subtilis. J. Bacteriol. 163:610-614.
32. Rehm, B. H. 2003. Polyester synthases: natural catalysts for plastics. Biochem. J. 376:15-33.
33. Saegusa, H., M. Shiraki, C. Kanai, and T. Saito. 2001. Cloning of an intracellular poly[D(-)-3-hydroxybutyrate] depolymerase gene from Ralstonia eutrepha H16 and characterization of the gene product. J. Bacteriol. 183:94-100.
34. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
35. Shine, J., and L. Dalgarno. 1974. The 3′-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc. Natl. Acad. Sci. USA 71:1342-1346.
36. Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
37. Tokiwa, Y., and B. P. Calabia. 2004. Degradation of microbial polyesters. Biotechnol. Lett. 26:1181-1189.
38. Wakisaka, Y., E. Masaki, and Y. Nishimoto. 1982. Formation of crystalline δ-endotoxin or poly-β-hydroxybutyric acid granules by asporogenous mutants of Bacillus thuringiensis. Appl. Environ. Microbiol. 43:1473-1480.
39. Williamson, D. H., J. Mellanby, and H. A. Krebs. 1962. Enzymic determination of D(-)-β-hydroxybutyric acid and acetoacetic acid in blood. Biochem. J. 82:90-97.
40. York, G. M., J. Lupberger, J. Tian, A. G. Lawrence, J. Stubbe, and A. J. Sinskey. 2003. Ralstonia eutropha H16 encodes two and possibly three intracellular poly[D-(-)-3-hydroxybutyrate] depolymerase genes. J. Bacteriol. 185:3788-3794.