Chaperone-assisted overexpression of an active D-carbamoylase from Agrobacterium tumefaciens AM 10

Protein Expression and Purification

Volumn 23, Issue 3, 2001, Pages 374-379

  Sareen, Dipti ^a,b   Sharma, Rakesh ^a,c   Vohra, Rakesh M ^a  

^a INSTITUTE OF MICROBIAL TECHNOLOGY   (India)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF DELHI   (India)

Author keywords

Agrobacterium; Chaperone; D carbamoylase; Disulfide; Folding; Inclusion body; Metal affinity; N carbamoyl amidohydrolase; Overexpression; Purification

Indexed keywords

AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; ESCHERICHIA COLI; RHIZOBIUM;

AMIDASE; CHAPERONIN; N CARBAMOYL D AMINO ACID AMIDOHYDROLASE; N-CARBAMOYL-D-AMINO ACID AMIDOHYDROLASE; RECOMBINANT PROTEIN;

AGROBACTERIUM TUMEFACIENS; ARTICLE; BACTERIUM TRANSFORMATION; BIOSYNTHESIS; CELL INCLUSION; COMPARATIVE STUDY; ENZYME ACTIVATION; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; PROTEIN FOLDING; SOLUBILITY; TEMPERATURE;

AGROBACTERIUM TUMEFACIENS; AMIDOHYDROLASES; CLONING, MOLECULAR; COMPARATIVE STUDY; ENZYME ACTIVATION; ESCHERICHIA COLI; GENE EXPRESSION; GROEL PROTEIN; GROES PROTEIN; INCLUSION BODIES; PROTEIN FOLDING; RECOMBINANT PROTEINS; SOLUBILITY; SUPPORT, NON-U.S. GOV'T; TEMPERATURE; TRANSFORMATION, BACTERIAL;

EID: 0035543195     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1532     Document Type: Article

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