Refolding and purification of bothropstoxin-I, a Lys49-phospholipase A2 homologue, expressed as inclusion bodies in escherichia coli

Protein Expression and Purification

Volumn 21, Issue 1, 2001, Pages 134-140

  Ward, R J ^a   De Oliveira, A H C ^a   Bortoleto, R K ^b   Rosa, J C ^a   Faca V M ^a   Greene, L J ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; AMINO ACID SUBSTITUTION; ASPARAGINE; BOTHROPSTOXIN; CELL DAMAGE; CELL INCLUSION; CELL MEMBRANE; CIRCULAR DICHROISM SPECTROSCOPY; ENZYMIC HYDROLYSIS; EXPRESSION VECTOR; GENETIC MARKER; GENETIC STRAIN; LIPOSOME; PHOSPHOLIPASE A2; RECOMBINANT PROTEIN; SNAKE VENOM;

BOTHROPS JARARACUSSUSSU; ESCHERICHIA COLI;

EID: 0034961960     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1353     Document Type: Article

References (26)

1. The substrate specificity of phospholipase A2
2. 2 superfamily of signal tranduction enzymes
3. Methylation of histidine-48 in pancreatic phospholipase A2: Role of histidine and calcium ion in the catalytic mechanism
4. Interfacial catalysis: The mechanism of phospholipase A2
5. Review article: Phospholipase A2 myotoxins from Bothrops snake venoms
6. The crystal structure of a lysine 49 phospholipase A2 from the venom of the cottonmouth snake at 2.0-Å resolution
7. Crystallographic and biochemical studies of the (inactive) Lys-49 phospholipase A2 from the venom of Agkistrodon piscivorus piscivorus
8. Structure of a calcium-independent phospholipase-like myotoxic protein from Bothrops asper venom
9. Structure and catalytic mechanism of secretory phospholipases A2
10. Phospholipase A2 - A structural review
11. The effect of myotoxins isolated from Bothrops snake venoms on multilamellar liposomes: Relationship to phospholipase A2, anticoagulant and myotoxic activities
12. Calcium ion independent membrane leakage induced by phospholipase-like myotoxins
13. Fractionation of Bothrops jararacussu snake venom: Partial chemical characterization and biological activity of bothropstoxin
14. 2
15. 2 homologue
16. Sequence of a cDNA encoding bothropstoxin I, a myotoxin from the venom of Bothrops jararacussu
17. Site-directed mutagenesis of virtually any plasmid by eliminating a unique site
18. Sensitive quantitative analysis of disulfide bonds in polypeptides and proteins
19. Measurement of protein using bicinchoninic acid
20. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
21. 2 activity by colorimetric assay using a pH indicator
22. 2 engineering: Structural and functional roles of the highly conserved active site residue aspartate-49
23. Regeneration of full enzymatic activity by reoxidation of reduced pancreatic phospholipase A2
24. Protein refolding at high concentration using size-exclusion chromatography
25. Inclusion body purification and protein refolding using microfiltration and size exclusion chromatography
26. Considerations of sample application and elution during size-exclusion chromatography-based protein refolding