Reduction of AHSP synthesis in hemin-induced K562 cells and EPO-induced CD34+ cells leads to α-globin precipitation, impairment of normal hemoglobin production, and increased cell death

Experimental Hematology

Volumn 36, Issue 3, 2008, Pages 265-272

  Pinho, Flavia Oliveira ^a   de Albuquerque, Dulcineia Martins ^a   Olalla Saad, Sara Teresinha ^a   Costa, Fernando Ferreira ^a  

^a UNIVERSITY OF CAMPINAS   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA GLOBIN; ALPHA HEMOGLOBIN STABILIZING PROTEIN; CHAPERONE; ERYTHROPOIETIN; HEMIN; HEMOGLOBIN; HEMOGLOBIN ALPHA CHAIN; HEMOGLOBIN CHAIN; MESSENGER RNA; PROTEIN DERIVATIVE; REACTIVE OXYGEN METABOLITE; SHORT HAIRPIN RNA; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; APOPTOSIS; ARTICLE; CELL DEATH; CELL DIFFERENTIATION; CELL LINE; CONTROLLED STUDY; ERYTHROID CELL; ERYTHROPOIESIS; EXPRESSION VECTOR; FLOW CYTOMETRY; GENE EXPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; HYPOTHESIS; IMMUNOFLUORESCENCE TEST; MOLECULAR CLONING; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA INTERFERENCE;

ANTIGENS, CD34; APOPTOSIS; BLOOD PROTEINS; CELL DEATH; CELL DIFFERENTIATION; CLONING, MOLECULAR; ERYTHROID PROGENITOR CELLS; ERYTHROPOIETIN; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; GENE EXPRESSION PROFILING; GLOBINS; HEMIN; HEMOGLOBINS; HUMANS; K562 CELLS; MOLECULAR CHAPERONES; REACTIVE OXYGEN SPECIES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA INTERFERENCE; RNA, MESSENGER; TRANSFECTION;

EID: 39149123488     PISSN: 0301472X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.exphem.2007.11.003     Document Type: Article

References (23)

1. Miele G., Manson J., and Clinton M. A novel erythroid-specific marker of transmissible spongiform encephalopathies. Nat Med 7 (2001) 361-364
2. Kihm A.J., Kong Y., Hong W., et al. An abundant erythroid protein that stabilizes free alpha-haemoglobin. Nature 417 6890 (2002) 758-763
3. Kong Y., Zhou S., Kihm A.J., et al. Loss of alpha-hemoglobin-stabilizing protein impairs erythropoiesis and exacerbates beta-thalassemia. J Clin Invest 114 (2004) 1457-1466
4. Scott M.D. H2O2 injury in beta thalassemic erythrocytes: protective role of catalase and the prooxidant effects of GSH. Free Radic Biol Med 40 (2006) 1264-1272
5. Gell D., Kong Y., Eaton S.A., Weiss M.J., and Mackay J.P. Biophysical characterization of the alpha-globin binding protein alpha-hemoglobin stabilizing protein. J Biol Chem 277 (2002) 40602-40609
6. dos Santos C.O., Duarte A.S., Saad S.T., and Costa F.F. Expression of alpha-hemoglobin stabilizing protein gene during human erythropoiesis. Exp Hematol 32 (2004) 157-162
7. Schrier S.L. Pathophysiology of thalassemia. Curr Opin Hematol 9 (2002) 123-126
8. Viprakasit V., Tanphaichitr V.S., Chinchang W., Sangkla P., Weiss M.J., and Higgs D.R. Evaluation of alpha hemoglobin stabilizing protein (AHSP) as a genetic modifier in patients with beta thalassemia. Blood 103 (2004) 3296-3299
9. dos Santos C.O., and Costa F.F. AHSP and beta-thalassemia: a possible genetic modifier. Hematology 10 (2005) 157-161
10. Lai M.I., Jiang J., Silver N., et al. Alpha-haemoglobin stabilizing protein is a quantitative trait gene that modifies the phenotype of beta-thalassaemia. Br J Haematol 133 (2006) 675-682
11. Sui G., Soohoo C., Affar E.B., et al. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc Natl Acad Sci U S A 99 (2002) 5515-5520
12. Hollanda L.M., Lima C.S., Cunha A.F., et al. An inherited mutation leading to production of only the short isoform of GATA-1 is associated with impaired erythropoiesis. Nat Genet 38 (2006) 807-812
13. Vandesompele J., De Preter K., Pattyn F., et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3 (2002) RESEARCH0034
14. Hsiao C.H., Li W., Lou T.F., Baliga B.S., and Pace B.S. Fetal hemoglobin induction by histone deacetylase inhibitors involves generation of reactive oxygen species. Exp Hematol 34 (2006) 264-273
15. Ghosh P., Besra S.E., Tripathi G., Mitra S., and Vedasiromoni J.R. Cytotoxic and apoptogenic effect of tea (Camellia sinensis var. assamica) root extract (TRE) and two of its steroidal saponins TS1 and TS2 on human leukemic cell lines K562 and U937 and on cells of CML and ALL patients. Leuk Res 30 (2006) 459-468
16. Iyamu W.E., Adunyah S.E., Fasold H., et al. Enhancement of hemoglobin and F-cell production by targeting growth inhibition and differentiation of K562 cells with ribonucleotide reductase inhibitors (didox and trimidox) in combination with streptozotocin. Am J Hematol 63 (2000) 176-183
17. Wei X., Runnels J.M., and Lin C.P. Selective uptake of indocyanine green by reticulocytes in circulation. Invest Ophthalmol Vis Sci 44 (2003) 4489-4496
18. Feng L., Gell D.A., Zhou S., et al. Molecular mechanism of AHSP-mediated stabilization of alpha-hemoglobin. Cell 119 (2004) 629-640
19. Zhou S., Olson J.S., Fabian M., Weiss M.J., and Gow A.J. Biochemical fates of alpha hemoglobin bound to alpha hemoglobin-stabilizing protein AHSP. J Biol Chem 281 43 (2006) 32611-33268
20. Baudin-Creuza V., Vasseur-Godbillon C., Pato C., Prehu C., Wajcman H., and Marden M.C. Transfer of human alpha- to beta-hemoglobin via its chaperone protein: evidence for a new state. J Biol Chem 279 (2004) 36530-36533
21. Gianni A.M., Presta M., Polli E., et al. Preferential induction of fetal versus embryonic globin chains in human leukemic cell lines. Leuk Res 6 (1982) 155-163
22. Wojda U., Leigh K.R., Njoroge J.M., et al. Fetal hemoglobin modulation during human erythropoiesis: stem cell factor has "late" effects related to the expression pattern of CD117. Blood 101 (2003) 492-497
23. Yu X., Kong Y., Dore L.C., et al. An erythroid chaperone that facilitates folding of alpha-globin subunits for hemoglobin synthesis. J Clin Invest 117 (2007) 1856-1865