The tumor suppressor gene DLEC1 is frequently silenced by DNA methylation in hepatocellular carcinoma and induces G1 arrest in cell cycle

Journal of Hepatology

Volumn 48, Issue 3, 2008, Pages 433-441

  Qiu, Guo Hua ^a,b   Salto Tellez, Manuel ^a   Ross, James A ^c   Yeo, Winnie ^d   Cui, Yan ^b,d   Wheelhouse, Nicholas ^c   Chen, George G ^e   Harrison, David ^c   Lai, Paul ^e   Tao, Qian ^b,d   Hooi, Shing Chuan ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b Johns Hopkins Singapore   (Singapore)

^c UNIVERSITY OF EDINBURGH   (United Kingdom)

^d CHINESE UNIVERSITY OF HONG KONG   (Hong Kong)

^e CHINESE UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

DLEC1; DNA methylation; G1 arrest; Hepatocellular carcinoma; Tumor suppressor gene

Indexed keywords

5 AZA 2' DEOXYCYTIDINE; DNA;

ARTICLE; CANCER CELL CULTURE; CANCER GROWTH; CANCER STAGING; CELL CYCLE ARREST; CELL CYCLE G1 PHASE; CELL GROWTH; CELL PROLIFERATION; CELL SIZE; COLONY FORMATION; CORRELATION ANALYSIS; DATA ANALYSIS; DLEC1 GENE; DNA METHYLATION; DOWN REGULATION; GENE EXPRESSION; GENE SILENCING; GENETIC STABILITY; HUMAN; HUMAN CELL; HUMAN TISSUE; LIVER CELL CARCINOMA; MAJOR CLINICAL STUDY; MOLECULAR CLONING; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; TUMOR SUPPRESSOR GENE;

CARCINOMA, HEPATOCELLULAR; CELL CYCLE; CELL LINE, TUMOR; CELL PROLIFERATION; CELL SIZE; CPG ISLANDS; DISEASE PROGRESSION; DNA METHYLATION; DNA, NEOPLASM; FEMALE; G1 PHASE; GENE SILENCING; HUMANS; LIVER NEOPLASMS; MALE; MIDDLE AGED; TUMOR SUPPRESSOR PROTEINS;

EID: 39149101438     PISSN: 01688278     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jhep.2007.11.015     Document Type: Article

References (28)

1. Macheiner D., Heller G., Kappel S., Bichler C., Stattner S., Ziegler B., et al. NORE1B, a candidate tumor suppressor, is epigenetically silenced in human hepatocellular carcinoma. J Hepatol 45 (2006) 81-89
2. Schagdarsurengin U., Wilkens L., Steinemann D., Flemming P., Kreipe H.H., Pfeifer G.P., et al. Frequent epigenetic inactivation of the RASSF1A gene in hepatocellular carcinoma. Oncogene 22 (2003) 1866-1871
3. Zhong S., Yeo W., Tang M.W., Wong N., Lai P.B., and Johnson P.J. Intensive hypermethylation of the CpG island of Ras association domain family 1A in hepatitis B virus-associated hepatocellular carcinomas. Clin Cancer Res 9 (2003) 3376-3382
4. Imreh S., Klein G., and Zabarovsky E.R. Search for unknown tumor-antagonizing genes. Genes Chromosomes Cancer 38 (2003) 307-321
5. Agathanggelou A., Cooper W.N., and Latif F. Role of the Ras-association domain family 1 tumor suppressor gene in human cancers. Cancer Res 65 (2005) 3497-3508
6. Pfeifer G.P., and Dammann R. Methylation of the tumor suppressor gene RASSF1A in human tumors. Biochemistry (Moscow) 70 (2005) 576-583
7. Ito M., Ito G., Kondo M., Uchiyama M., Fukui T., Mori S., et al. Frequent inactivation of RASSF1A, BLU, and SEMA3B on 3p21. 3 by promoter hypermethylation and allele loss in non-small cell lung cancer. Cancer Lett 225 (2005) 131-139
8. Tomizawa Y., Sekido Y., Kondo M., Gao B., Yokota J., Roche J., et al. Inhibition of lung cancer cell growth and induction of apoptosis after reexpression of 3p21. 3 candidate tumor suppressor gene SEMA3B. Proc Natl Acad Sci USA 98 (2001) 13954-13959
9. Qiu G.H., Tan L.K., Loh K.S., Lim C.Y., Srivastava G., Tsai S.T., et al. The candidate tumor suppressor gene BLU, located at the commonly deleted region 3p21.3, is an E2F-regulated, stress-responsive gene and inactivated by both epigenetic and genetic mechanisms in nasopharyngeal carcinoma. Oncogene 23 (2004) 4793-4806
10. Lerman M.I., and Minna J.D. The 630-kb lung cancer homozygous deletion region on human chromosome 3p21.3: identification and evaluation of the resident candidate tumor suppressor genes. The International Lung Cancer Chromosome 3p21.3 Tumor Suppressor Gene Consortium. Cancer Res 60 (2000) 6116-6133
11. Protopopov A., Kashuba V., Zabarovska V.I., Muravenko O.V., Lerman M.I., Klein G., et al. An integrated physical and gene map of the 3.5-Mb chromosome 3p21.3 (AP20) region implicated in major human epithelial malignancies. Cancer Res 63 (2003) 404-412
12. Rauch T., Li H., Wu X., and Pfeifer G.P. MIRA-assisted microarray analysis, a new technology for the determination of DNA methylation patterns, identifies frequent methylation of homeodomain-containing genes in lung cancer cells. Cancer Res 66 (2006) 7939-7947
13. Daigo Y., Nishiwaki T., Kawasoe T., Tamari M., Tsuchiya E., and Nakamura Y. Molecular cloning of a candidate tumor suppressor gene, DLC1, from chromosome 3p21.3. Cancer Res 59 (1999) 1966-1972
14. Kwong J., Lee J.Y., Wong K.K., Zhou X., Wong D.T., Lo K.W., et al. Candidate tumor-suppressor gene DLEC1 is frequently downregulated by promoter hypermethylation and histone hypoacetylation in human epithelial ovarian cancer. Neoplasia 8 (2006) 268-278
15. Kwong J., Chow L.S., Wong A.Y., Hung W.K., Chung G.T., To K.F., et al. Epigenetic inactivation of the deleted in lung and esophageal cancer 1 gene in nasopharyngeal carcinoma. Genes Chromosomes Cancer 46 (2007) 171-180
16. Qiu G.H., Xie H., Wheelhouse N., Harrison D., Chen G.G., Salto-Tellez M., et al. Differential expression of hDAB2IPA and hDAB2IPB in normal tissues and promoter methylation of hDAB2IPA in hepatocellular carcinoma. J Hepatol 46 (2007) 655-663
17. Herman J.G., Graff J.R., Myohanen S., Nelkin B.D., and Baylin S.B. Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci USA 93 (1996) 9821-9826
18. Tao Q., Huang H., Geiman T.M., Lim C.Y., Fu L., Qiu G.H., et al. Defective de novo methylation of viral and cellular DNA sequences in ICF syndrome cells. Hum Mol Genet 11 (2002) 2091-2102
19. Toyota M., Sasaki Y., Satoh A., Ogi K., Kikuchi T., Suzuki H., et al. Epigenetic inactivation of CHFR in human tumors. Proc Natl Acad Sci USA 100 (2003) 7818-7823
20. Rabizadeh S., Xavier R.J., Ishiguro K., Bernabeortiz J., Lopez-Ilasaca M., Khokhlatchev A., et al. The scaffold protein CNK1 interacts with the tumor suppressor RASSF1A and augments RASSF1A-induced cell death. J Biol Chem 279 (2004) 29247-29254
21. Fingar D.C., Salama S., Tsou C., Harlow E., and Blenis J. Mammalian cell size is controlled by mTOR and its downstream targets S6K1 and 4EBP1/eIF4E. Genes Dev 16 (2002) 1472-1487
22. Zabarovsky E.R., Lerman M.I., and Minna J.D. Tumor suppressor genes on chromosome 3p involved in the pathogenesis of lung and other cancers. Oncogene 21 (2002) 6915-6935
23. Kondo M., Ji L., Kamibayashi C., Tomizawa Y., Randle D., Sekido Y., et al. Overexpression of candidate tumor suppressor gene FUS1 isolated from the 3p21.3 homozygous deletion region leads to G1 arrest and growth inhibition of lung cancer cells. Oncogene 20 (2001) 6258-6262
24. Kashuba V.I., Li J., Wang F., Senchenko V.N., Protopopov A., Malyukova A., et al. RBSP3 (HYA22) is a tumor suppressor gene implicated in major epithelial malignancies. Proc Natl Acad Sci USA 101 (2004) 4906-4911
25. Litchfield D.W. Protein kinase CK2: structure, regulation and role in cellular decisions of life and death. Biochem J 369 (2003) 1-15
26. Pepperkok R., Lorenz P., Ansorge W., and Pyerin W. Casein kinase II is required for transition of G0/G1, early G1, and G1/S phases of the cell cycle. J Biol Chem 269 (1994) 6986-6991
27. Jans D.A., and Jans P. Negative charge at the casein kinase II site flanking the nuclear localization signal of the SV40 large T-antigen is mechanistically important for enhanced nuclear import. Oncogene 9 (1994) 2961-2968
28. Fontes M.R., Teh T., Toth G., John A., Pavo I., Jans D.A., et al. Role of flanking sequences and phosphorylation in the recognition of the simian-virus-40 large T-antigen nuclear localization sequences by importin-alpha. Biochem J 375 (2003) 339-349