Cryo-EM reveals an active role for aminoacyl-tRNA in the accommodation process

EMBO Journal

Volumn 21, Issue 13, 2002, Pages 3557-3567

  Valle, Mikel ^a   Sengupta, Jayati ^a   Swami, Neil K ^a   Grassucci, Robert A ^a   Burkhardt, Nils ^a   Nierhaus, Knud H ^a   Agrawal, Rajendra K ^a   Frank, Joachim ^a  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

Decoding; Electron microscopy; Elongation factor Tu; Ribosome; tRNA

Indexed keywords

AMINOACYL TRANSFER RNA; ELONGATION FACTOR TU; GUANOSINE TRIPHOSPHATE; MOCIMYCIN;

ANTICODON; ARTICLE; CODON; CRYOELECTRON MICROSCOPY; ESCHERICHIA COLI; HYDROLYSIS; MOLECULAR RECOGNITION; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN SYNTHESIS; RIBOSOME; SIGNAL TRANSDUCTION;

ANTICODON; CODON; CRYOELECTRON MICROSCOPY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATE; IMAGE PROCESSING, COMPUTER-ASSISTED; MACROMOLECULAR SUBSTANCES; MODELS, MOLECULAR; NUCLEIC ACID CONFORMATION; PEPTIDE CHAIN ELONGATION, TRANSLATIONAL; PEPTIDE ELONGATION FACTOR TU; PROTEIN CONFORMATION; PYRIDONES; RIBOSOMES; RNA, TRANSFER; RNA, TRANSFER, AMINO ACYL; RNA, TRANSFER, PHE; STRUCTURE-ACTIVITY RELATIONSHIP;

ESCHERICHIA COLI;

EID: 0036646535     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/cdf326     Document Type: Article

References (65)

1. Mutations to kirromycin resistance occur in the interface of domains I and II of EF-Tu-GTP
2. An α to β conformational switch in EF-Tu
3. Visualization of elongation factor G on the Escherichia coli 70S ribosome: The mechanism of translocation
4. EF-G-dependent GTP hydrolysis induces translocation accompanied by large conformational changes in the 70S ribosome
5. Visualization of tRNA movements on the Escherichia coli ribosome during the elongation cycle
6. Studies of elongation factor G-dependent tRNA translocation by three-dimensional cryo-electron microscopy
7. Localization of L11 protein on the ribosome and elucidation of its involvement in EF-G-dependent translocation
8. The complete atomic structure of the large ribosomal subunit at 2.4 Å resolution
9. Crystal structure of active elongation factor Tu reveals major domain rearrangements
10. Preparation of functional ribosomal complexes and the effect of buffer conditions on tRNA positions observed by cryoelectron microscopy
11. Ribbons
12. Crystal structure of an initiation factor bcund to the 30S ribosomal subunit
13. X-ray structures of 70S ribosome functional complexes
14. Effects of nucleotide-and aurodox-induced changes in elongation factor Tu conformation upon its interaction with aminoacyl-tRNA. A fluorescence study
15. Asp: Atomic coordinates
16. Conformational changes in tRNA: Consequences of aminoacylation and codon-anticodon recognition
17. Classification of macromolecular assemblies studied as 'single particles
18. On the correction of the contrast transfer function in biological electron microscopy
19. Three-dimensional cryo-elcctron microscopy of ribosomes
20. Solution structure of E. Coli 70S ribosome at 11.5 Å resolution
21. Significance of the third tRNA binding site, the E site, on E. Coli ribosomes for the accuracy of translation: An occupied E site prevents the binding of non-cognate aminoacyl-tranfer RNA to the A site
22. Evidence that the G2661 region of 23S rRNA is located at the ribosomal binding sites of both elongation factors
23. Improved methods for binding protein models in electron density maps and the location of errors in these models
24. The role of guanosine-5′-triphosphate in polypeptide chain elongation
25. The crystal structure of elongation factor EF-Tc from Thermus aquaticus in the GTP conformation
26. Automatic particle picking from electron micrographs
27. A conformational switch in Escherichia coli 16S ribosomal RNA during the decoding of messenger RNA
28. Met and fitting of L1 protein
29. The structural and functional basis for the kirromycin resistance of mutant EF-Tu species in E.coli
30. Intermediate states in the movement of transfer RNA in the ribosome
31. Interaction of elongation factors EF-G and EF-Tu with a conserved loop in 23S rRNA
32. The structure of yeast tRNA (Asp). A model for tRNA interacting with messenger RNA
33. Asp, a model for tRNA-mRNA recognition
34. Phe, EF-TU and a GTP analog
35. Molecular mimicry
36. Recognition of cognate transfer RNA by the 30S ribosomal subunit
37. Structure of keyhole limpet hemocyanin type 1 (KLH1) at 15 A resolution by electron cryomicroscopy and angular reconstitution
38. Mechanism of action of kirromycin-like antibiotics
39. The ribosome at improved resolution: New techniques for merging and orientation refinement in 3D cryo-electron microscopy of biological particles
40. Intact aminoacyl-tRNA is required to trigger GTP hydrolysis by elongation factor Tu on the ribosome
41. Helix unwinding in the effector region of elongation factor EF-Tu-GDP
42. The 530 loop of 16S rRNA: A signal to EF-Tu?
43. Fidelity of aminoacyl-tRNA selection on the ribosome: Kinetic and structural mechanism
44. Transient conformational states of aminoacyl-tRNA during ribosome binding catalyzed by elongation factor Tu
45. Codon-dependent conformational change of elongation factor Tu preceding GTP hydrolysis on the ribosome
46. An A to U transversion at position 1067 of 23S rRNA from Escherichia coli impairs EF-Tu and EF-G function
47. Induced structural transitions in tRNA
48. The transorientation hypothesis for codon recognition during protein synthesis
49. Crystal structure of intact elongation factor EF-Tu from Escherichia coli in GDP conformation at 2.05 Å resolution
50. Visualization of elongation factor Tu on Escherichia coli ribosome
51. Large scale movement of elongation factor G and extensive conformational change of the ribosome during translocation
52. Self-coded 3′-extension of run-off transcripts produces aberrant products during in vitro transcription with T7 RNA polymerase
53. Movement of the decoding region of the 16S ribosomal RNA accompanies tRNA translocation
54. Electron microscopy and computer image averaging of ice-embedded large ribosomal subunits from Escherichia coli
55. A detailed view of a ribosomal active site: The structure of the L11-RNA complex
56. Structure of the 30S ribosomal subunit
57. Molecular mechanics of translation: A reciprocating ratchet mechanism
58. Translation: In retrospect and prospect
59. Mechanism of the inhibition of protein synthesis by kirromycin
60. Ribotoxin recognition of ribosomal-RNA and a proposal for the mechanism of translocation
61. Domains motions of EF-G bound to the ribosome: Insights from a hand-shaking between multi-resolution structures
62. tRNA on the ribosome: A waggle theory
63. The path of the messenger RNA through the ribosome