A second generation apparatus for time-resolved electron cryo-microscopy using stepper motors and electrospray

Journal of Structural Biology

Volumn 144, Issue 1-2, 2003, Pages 246-252

  White, H D ^a   Thirumurugan, K ^b   Walker, M L ^b   Trinick, J ^b  

^a EASTERN VIRGINIA MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF LEEDS   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AEROSOL; APPARATUS; ARTICLE; CONFORMATION; CRYOELECTRON MICROSCOPY; ELECTRICITY; ELECTRON MICROSCOPE; FILM; FREEZING; MACROMOLECULE; PRIORITY JOURNAL; REACTION ANALYSIS; TIME; TURBULENT FLOW;

EID: 0344983330     PISSN: 10478477     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jsb.2003.09.027     Document Type: Article

References (12)

1. Berriman J., Unwin N. Analysis of transient structures by cryo-microscopy combined with rapid mixing of spray droplets. Ultramicroscopy. 56:1994;241-252.
2. Cole R.B. Some tenets pertaining to electrospray ionization mass spectrometry. J. Mass Spectrom. 35:2000;763-772.
3. Fenn J.B., Mann M., Meng C.K., Wong S.F., Whitehouse C.M. Electrospray ionization for mass-spectrometry of large biomolecules. Science. 246:1989;64-71.
4. Kebarle P. A brief overview of the present status of the mechanisms involved in electrospray mass spectrometry. J. Mass Spectrom. 35:2000;804-817.
5. Moffat K., Henderson R. Freeze trapping of reaction intermediates. Curr. Opin. Struct. Biol. 5:1995;656-663.
6. Taylor G.I. Proc. Roy. Soc. Lond. A Mat. 280:1964;383.
7. Trinick J., Cooper J. Concentration of solutes during preparation of aqueous suspensions for cryo-electron microscopy. J. Microsc. 159:1990;215-222.
8. Walker M., Trinick J., White H. Millisecond time resolution electron cryo-microscopy of the M-ATP transient kinetic state of the acto-myosin ATPase. Biophys. J. 68:1995;87s-91s.
9. Walker M., Zhang X.Z., Jiang W., Trinick J., White H.D. Observation of transient disorder during myosin subfragment-1 binding to actin by stopped-flow fluorescence and millisecond time resolution electron cryomicroscopy: evidence that the start of the crossbridge power stroke in muscle has variable geometry. Proc. Natl. Acad. Sci. (USA). 96:1999;465-470.
10. White H.D., Walker M.L., Trinick J. A computer controlled spray-freezing apparatus for millisecond time-resolution electron cryo-microscopy. J. Struct. Biol. 121:1998;306-313.
11. Whitehouse C.M., Dreyer R.N., Yamashita M., Fenn J.B. Electrospray interface for liquid chromatographs and mass spectrometers. Anal. Chem. 57:1985;675-679.
12. Yamashita M., Fenn J.B. Electrospray ion-source - another variation on the free-jet theme. J. Phys. Chem. 88:1984;4451-4459.