Increased levels of numerical chromosome aberrations after in vitro exposure of human peripheral blood lymphocytes to radiofrequency electromagnetic fields for 72 hours

Radiation Research

Volumn 169, Issue 1, 2008, Pages 28-37

  Mazor, Ronit ^a   Korenstein Ilan, Avital ^a   Barbul, Alexander ^a   Eshet, Yael ^a   Shahadi, Avi ^a   Jerby, Eli ^a   Korenstein, Rafi ^a  

^a TEL AVIV UNIVERSITY   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

ABSORPTION; ADULT; ANEUPLOIDY; ARTICLE; CHROMOSOME 1; CHROMOSOME 10; CHROMOSOME 11; CHROMOSOME 17; CHROMOSOME ABERRATION; CLINICAL ARTICLE; ELECTROMAGNETIC FIELD; FLUORESCENCE IN SITU HYBRIDIZATION; GENOMIC INSTABILITY; HEALTH HAZARD; HUMAN; HUMAN CELL; IMAGE ANALYSIS; IN VITRO STUDY; LONG TERM EXPOSURE; MALE; NORMAL HUMAN; PERIPHERAL LYMPHOCYTE; PRACTICE GUIDELINE; PRIORITY JOURNAL; RADIATION EXPOSURE; RADIOFREQUENCY RADIATION; TEMPERATURE; CELL CULTURE; CELL PROLIFERATION; CYTOLOGY; GENETICS; HUMAN CHROMOSOME; LYMPHOCYTE; METABOLISM; RADIOMETRY; TIME;

ADULT; ANEUPLOIDY; CELL PROLIFERATION; CELLS, CULTURED; CHROMOSOMES, HUMAN; ELECTROMAGNETIC FIELDS; HUMANS; LYMPHOCYTES; MALE; RADIO WAVES; RADIOMETRY; TEMPERATURE; TIME FACTORS;

EID: 39049083380     PISSN: 00337587     EISSN: None     Source Type: Journal    
DOI: 10.1667/RR0872.1     Document Type: Article

References (44)

1. D. Brusick, R. Albertini, D. McRee, D. Peterson, G. Williams, P. Hanawalt and J. Preston, Genotoxicity of radiofrequency radiation. DNA/Genetox Expert Panel. Environ. Mol. Mutagen. 32, 1-16 (1998).
2. Vijayalaxmi and G. Obe, Controversial cytogenetic observations in mammalian somatic cells exposed to radiofrequency radiation. Radiat. Res. 162, 481-496 (2004).
3. R. Li, A. Sonik, R. Stindl, D. Rasnick and P. Duesberg, Aneuploidy vs. gene mutation hypothesis of cancer: recent study claims mutation but is found to support aneuploidy. Proc. Natl. Acad. Sci. USA 97, 3236-3241 (2000).
4. P. Duesberg and R. Li, Multistep carcinogenesis: a chain reaction of aneuploidizations. Cell Cycle 2, 202-210 (2003).
5. P. Duesberg and D. Rasnick, Aneuploidy, the somatic mutation that makes cancer a species of its own. Cell Motil. Cytoskeleton 47, 81-107 (2000).
6. P. Dey, Aneuploidy and malignancy: an unsolved equation. J. Clin. Pathol. 57, 1245-1249 (2004).
7. D. Grimm, Genetics. Disease backs cancer origin theory. Science 306, 389 (2004).
8. D. Xu and R. L. Juliano, Epigenetic modulation of gene expression in mammalian cells. Crit. Rev. Eukaryot. Gene Expr. 15, 93-101 (2005).
9. P. A. Jones, Overview of cancer epigenetics. Semin. Hematol. 42, S3-S8 (2005).
10. A. Korenstein-Ilan, A. Amiel, S. Lalezari, M. Lishner and L. Avivi, Allele-specific replication associated with aneuploidy in blood cells of patients with hematologic malignancies. Cancer Genet. Cytogenet. 139, 97-103 (2002).
11. P. Duesberg, A. Fabarius and R. Hehlmann, Aneuploidy, the primary cause of the multilateral genomic instability of neoplastic and pre-neoplastic cells. IUBMB Life 56, 65-81 (2004).
12. S. Hanks, K. Coleman, S. Reid, A. Plaja, H. Firth, D. Fitzpatrick, A. Kidd, K. Mehes, R. Nash and Rahamn, Constitutional aneuploidy and cancer predisposition caused by biallelic mutations in BUB1B. Nat. Genet. 36, 1159-1161 (2004).
13. P. Duesberg, Are centrosomes or aneuploidy the key to cancer? Science 284, 2091-2092 (1999).
14. C. Lengauer and Z. Wang, From spindle checkpoint to cancer. Nat. Genet. 36, 1144-1145 (2004).
15. A. Musio, D. Zambroni, P. Vezzoni and T. Mariani, Chromosomes, genes, and cancer breakpoints. Cancer Genet. Cytogenet. 139, 141-142 (2002).
16. D. Pinkel, T. Straume and J. W. Gray, Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc. Natl. Acad. Sci. USA 83, 2934-2938 (1986).
17. D. A. Eastmond and D. Pinkel, Detection of aneuploidy and aneuploidy-inducing agents in human lymphocytes using fluorescence in situ hybridization with chromosome-specific DNA probes. Mutat. Res. 234, 303-318 (1990).
18. A. B. Mukherjee, V. V. Murty and R. S. Chaganti, Detection of cell-cycle stage by fluorescence in situ hybridization: its application in human interphase cytogenetics. Cytogenet. Cell Genet. 61, 91-94 (1992).
19. T. Litmanovitch, M. M. Altaras, A. Dotan and L. Avivi, Asynchronous replication of homologous alpha-satellite DNA loci in man is associated with nondisjunction. Cytogenet. Cell Genet. 81, 26-35 (1998).
20. A. Nagler, A. Korenstein-Ilan, A. Amiel and L. Avivi, Granulocyte colony-stimulating factor generates epigenetic and genetic alterations in lymphocytes of normal volunteer donors of stem cells. Exp. Hematol. 32, 122-130 (2004).
21. M. Mashevich, D. Folkman, A. Kesar, A. Barbul, R. Korenstein, E. Jerby and L. Avivi, Exposure of human peripheral blood lymphocytes to electromagnetic fields associated with cellular phones leads to chromosomal instability. Bioelectromagnetics 24, 82-90 (2003).
22. M. J. Barch, T. Knutsen and J. L. Spurbeck, Eds., The AGT Cytogenetics Laboratory Manual. Lippincott-Raven, Philadelphia, 1997.
23. T. Bovery, Zur frage der Entsehung mailigner Tumoren. Gustav Fischer Verlag, 1914.
24. L. A. Loeb, Mutator phenotype may be required for multistage carcinogenesis. Cancer Res. 51, 3075-3079 (1991).
25. R. A. Bennett, H. Izumi and K. Fukasawa, Induction of centrosome amplification and chromosome instability in p53-null cells by transient exposure to subtoxic levels of S-phase-targeting anticancer drugs. Oncogene 23, 6823-6829 (2004).
26. P. Duesberg, R. Li and D. Rasnick, Aneuploidy approaching a perfect score in predicting and preventing cancer: highlights from a conference held in Oakland, CA in January, 2004. Cell Cycle 3, 823-828 (2004).
27. P. Duesberg, R. Stindl and R. Hehlmann, Explaining the high mutation rates of cancer cells to drug and multidrug resistance by chromosome reassortments that are catalyzed by aneuploidy. Proc. Natl. Acad. Sci. USA 97, 14295-14300 (2000).
28. A. Rökman, T. Ikonen, E. H. Seppälä, N. Nupponen, V. Autio, N. Mononen, J. Bailey-Wilson, J. Trent, J. Carpten and J. Schleutker, Germline alterations of the RNASEL gene, a candidate HPC1 gene at 1q25, in patients and families with prostate cancer. Am J. Hum. Genet. 70, 1299-1304 (2002).
29. L. Zhang, Q. Yu, J. He and X. Zha, Study of the PTEN gene expression and FAK phosphorylation in human hepatocarcinoma tissues and cell lines. Mol. Cell. Biochem. 262, 25-33 (2004).
30. G. Rotman and Y. Shiloh, ATM: from gene to function. Hum. Mol. Genet. 7, 1555-1563 (1998).
31. A. J. Levine, p53, the cellular gatekeeper for growth and division. Cell 88, 323-331 (1997).
32. A. de la Chapelle and P. Peltomaki, The genetics of hereditary common cancers. Curr. Opin. Genet. Dev. 8, 298-303 (1998).
33. D. H. Teng, R. Hu, H. Lin, T. Davis, D. Iliev, C. Frye, B. Swedlund, K. L. Hansen, V. L. Vinson and P. A. Steck, MMAC1/PTEN mutations in primary tumor specimens and tumor cell lines. Cancer Res. 57, 5221-5225 (1997).
34. F. M. van de Rijke, H. Vrolijk, W. Sloos, H. J. Tanke and A. K. Raap, Sample preparation and in situ hybridization techniques for automated molecular cytogenetic analysis of white blood cells. Cytometry 24, 151-157 (1996).
35. L. Zhang, N. Rothman, Y. Wang, R. B. Hayes, S. Yin, N. Titenko-Holland, M. Dosemeci, Y. Z. Wang, P. Kolachana and M. T. Smith, Benzene increases aneuploidy in the lymphocytes of exposed workers: a comparison of data obtained by fluorescence in situ hybridization in interphase and metaphase cells. Environ. Mol. Mutagen. 34, 260-268 (1999).
36. D. A. Eastmond, M. Schuler and D. S. Rupa, Advantages and limitations of using fluorescence in situ hybridization for the detection of aneuploidy in interphase human cells. Mutat. Res. 348, 153-162 (1995).
37. L. Zhang, W. Yang, A. E. Hubbard and M. T. Smith, Nonrandom aneuploidy of chromosomes 1, 5, 6, 7, 8, 9, 11, 12, and 21 induced by the benzene metabolites hydroquinone and benzenetriol. Environ. Mol. Mutagen. 45, 388-396 (2005).
38. S. Munne, M. Bahce, M. Sandalinas, T. Escudero, C. Marquez, E. Velilla, P. Colls, M. Oter, M. Alikani and J. Cohen, Differences in chromosome susceptibility to aneuploidy and survival to first trimester. Reprod. Biomed. Online 8, 81-90 (2004).
39. C. K. Chou and M. Swicord, Comment on "Exposure of human peripheral blood lymphocytes to electromagnetic fields associated with cellular phones leads to chromosomal instability," by Mashevich et al. Bioelectromagnetics 2003;24(2):82-90. Bioelectromagnetics 24, 582 (2003).
40. A. Korenstein and R. Barbul, Reply to the comment on "exposure of human peripheral blood lymphocytes to electromagnetic fields associated with cellular phones leads to chromosomal instability. Bioelectromagnetics 24, 583-585 (2003).
41. E. Diem, C. Schwarz, F. Adlkofer, O. Jahn and H. Rudiger, Non-thermal DNA breakage by mobile-phone radiation (1800 MHz) in human fibroblasts and in transformed GFSH-R17 rat granulosa cells in vitro. Mutat. Res. 583, 178-183 (2005).
42. S. Velizarov, P. Raskmark and S. Kwee, The effects of radiofrequency fields on cell proliferation are non-thermal. Bioelectrochem. Bioenerg. 48, 177-180 (1999).
43. D. Leszczynski, S. Joenvaara, J. Reivinen and R. Kuokka, Non-thermal activation of the hsp27/p38MAPK stress pathway by mobile phone radiation in human endothelial cells: molecular mechanism for cancer- and blood-brain barrier-related effects. Differentiation 70, 120-129 (2002).
44. Guidelines for limiting exposure to time-varying electric, magnetic, and electromagnetic fields (up to 300 GHz). International Commission on Non-Ionizing Radiation Protection. Health Phys. 74, 494-522 (1998).