Extraction of yeast alcohol dehydrogenase using reversed micelles formed with CTAB

Journal of Chemical Technology and Biotechnology

Volumn 75, Issue 9, 2000, Pages 798-802

  Paul Chen, J ^a   Wu, Shunnian ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Alcohol dehydrogenase; CTAB; Protein purification; Reversed micelles

Indexed keywords

ALCOHOL DEHYDROGENASE; CETYLTRIMETHYLAMMONIUM BROMIDE; LARGE MOLECULAR WEIGHT PROTEINS; PHASE TRANSFER METHOD;

EXTRACTION; MICELLES; PURIFICATION; YEAST;

PROTEINS;

ALCOHOL DEHYDROGENASE; CETRIMIDE;

ARTICLE; ENZYME ACTIVITY; EXTRACTION; IONIC STRENGTH; MICELLE; NONHUMAN; PH; PROTEIN PURIFICATION; SACCHAROMYCES CEREVISIAE;

EID: 0034284113     PISSN: 02682575     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-4660(200009)75:9<798::AID-JCTB282>3.0.CO;2-P     Document Type: Article

References (18)

1. Isolating enzyme by reversed micelles
2. Reverse micelles as hosts for proteins and small molecules
3. Protein extraction using reverse micelles: Kinetics of protein partitioning
4. Extraction of enzymes by reversed micelles
5. Reversed micellar extraction of proteins
6. Protein extraction by reversed micelles using dioleyl phosphoric acid
7. A reversed micellar system using mixed surfacants of sodium bis(ethylhexyl) sulfsuccinate and di(2-ethylhexyl) phosphoric acid for extraction of proteins
8. Purification of intracellular enzymes from whole bacterial cells using reversed micelles
9. Effective purification method of large molecular weight proteins using conventional AOT reverse micelles
10. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
11. Zinc, a component of yeast alcohol dehydrogenase
12. Affinity thermoprecipitation of yeast alcohol dehydrogenase through metal ion-promoted binding with Eudragit-bound Cibacron Blue 3 GA
13. Purification of the Penicillium citrinum lipase using AOT reversed micelles
14. Studies on the purification of peroxidase from horseradish roots using reverse micelles
15. Liquid-liquid extraction of proteins with reversed micelles
16. Interfacial transport processes in the reversed micellar extraction of proteins