Immobilised metal ion affinity chromatography purification of alcohol dehydrogenase from baker's yeast using an expanded bed adsorption system

Journal of Chromatography A

Volumn 840, Issue 2, 1999, Pages 195-204

  Willoughby, N A ^a   Kirschner, T ^a   Smith, M P ^a   Hjorth, R ^b   Titchener Hooker, N J ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b GE Healthcare Bio Sciences AB   (Sweden)

Author keywords

Alcohol dehydrogenase; Enzymes; Expanded bed adsorption; Immobilised metal ion affinity chromatography; Yeast

Indexed keywords

ALCOHOL DEHYDROGENASE; EDETIC ACID; METAL ION;

ABSORPTION; AFFINITY CHROMATOGRAPHY; ARTICLE; CONTROLLED STUDY; IMMOBILIZATION; METHODOLOGY; NONHUMAN; PRIORITY JOURNAL; PURIFICATION; SACCHAROMYCES CEREVISIAE;

ADSORPTION; ALCOHOL DEHYDROGENASE; CHROMATOGRAPHY, AFFINITY; EDETIC ACID; HISTIDINE; IMIDAZOLES; METALS; SACCHAROMYCES CEREVISIAE; SOLVENTS; SPECTROPHOTOMETRY, ULTRAVIOLET;

EID: 0032953584     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0021-9673(99)00188-0     Document Type: Article

References (24)

1. Datar R.V., Rosen C.G. Asenjo J.A. Separation Processes in Biotechnology. 1990;741 Marcel Dekker, New York.
2. Fish N.M., Lilly M.D. Bio/Tech. 2:1984;623.
3. Chang Y.K., McCreath G.E., Draeger N.M., Chase H.A. Trans IChemE. 71:(Part B):1993;299.
4. Chase H.A. Tibtech. 12:1994;29.
5. Thömmes J. Adv. Biochem. Eng./Biotechnol. 58:1997;186.
6. Hjorth R. Tibtech. 15:1997;230.
7. Chang Y.K., Chase H.A. Biotech. Bioeng. 49:1996;204.
8. Chang Y.K., McCreath G.E., Chase H.A. Biotech. Bioeng. 48:1995;355.
9. Porath J., Carlsson J., Isson I., Belfrage G. Nature. 258:1975;598.
10. Chang Y.K., McCreath G.E., Draeger N.M., Chase H.A. TransIChemE. 71:(Part B):1993;299.
11. Hannson M., Stahl S., Hjorth R., Uhlen M., Moks T. Bio/Tech. 12:1994;285.
12. McCreath G.E., Chase H.A., Owen R.O., Lowe C.R. Biotech. Bioeng. 48:1995;341.
13. Pessoa A., Hartmann R., Vitolo M., Hustedt H. J. Biotech. 51:1996;89.
14. Degener A., Belew M., Velander W.H. J. Chromatogr. A. 799:1998;125.
15. Johansson H.J., Jaegersten C., Shiloach J. J. Biotech. 48:1996;9.
16. A. Suding, M. Tomusiak, Abs. A.C.S. 205 meeting, Pt. 2 BTEC61, 1993.
17. R.R. Beitle, M.M. Ataai, AIChE Symp. Ser. 290 Vol. 88 (1991) 34.
18. Arnold F.H. Bio/Tech. 9:1991;151.
19. Angal S., Dean P.D.G. Harris E.L.V., Angal S. Protein Purification Methods - A Practical Approach. 1st ed :1989;245 IRL, Oxford.
20. M.P. Smith, Ph.D. Thesis, University College London, 1997, p. 224.
21. Andersson L., Sulkowski E. J. Chromatogr. 604:1992;13.
22. H.O. Bergmeyer, Methods of Enzymic Analysis, Verlag Chemie, Weinheim, 1979.
23. Bradford M. Anal. Biochem. 72:1976;248.
24. Streamline 50 Operators Handbook, Pharmacia Biotech, Uppsala, 1993, p. 16.