Anti-inflammatory activity of β2-agonists in primary lung epithelial cells is independent of glucocorticoid receptor

European Respiratory Journal

Volumn 30, Issue 5, 2007, Pages 848-856

  Loven J ^a   Svitacheva, N ^a   Jerre, A ^a   Miller Larsson, A ^a   Korn, S H ^a  

^a ASTRAZENECA R AND D   (Sweden)

Author keywords

Anti inflammatory; Glucocorticoid receptor; Granulocyte macrophage colony stimulating factor; Long acting 2 agonists; Small interfering RNA; Western blot

Indexed keywords

BUDESONIDE; FORMOTEROL; GLUCOCORTICOID RECEPTOR; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; SALMETEROL; SMALL INTERFERING RNA; TUMOR NECROSIS FACTOR ALPHA;

ARTICLE; ASTHMA; CHRONIC OBSTRUCTIVE LUNG DISEASE; CONTROLLED STUDY; DRUG EFFECT; EPITHELIUM CELL; GENE ACTIVITY; HUMAN; HUMAN CELL; LUNG ALVEOLUS EPITHELIUM; PRIORITY JOURNAL; WESTERN BLOTTING;

ADRENERGIC BETA-AGONISTS; ALBUTEROL; ASTHMA; BLOTTING, WESTERN; BRONCHI; BRONCHODILATOR AGENTS; BUDESONIDE; CELLS, CULTURED; EPITHELIAL CELLS; ETHANOLAMINES; GENES, REPORTER; GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR; HUMANS; RECEPTORS, GLUCOCORTICOID; SIGNAL TRANSDUCTION; TUMOR NECROSIS FACTOR-ALPHA;

EID: 38849123625     PISSN: 09031936     EISSN: None     Source Type: Journal    
DOI: 10.1183/09031936.00129606     Document Type: Article

References (31)

1. Pauwels RA, Lofdahl CG, Postma DS, et al. Effect of inhaled formoterol and budesonide on exacerbations of asthma. Formoterol and Corticosteroids Establishing Therapy (FACET) International Study Group. N Engl J Med 1997; 337: 1405-1411.
2. 2 agonist, inhaled corticosteroid and their combination in the treatment of asthma. Thorax 1999; 54: 482-487.
3. Woolcock A, Lundback B, Ringdal N, Jacques LA. Comparison of addition of salmeterol to inhaled steroids with doubling of the dose of inhaled steroids. Am J Respir Crit Care Med 1996; 153: 1481-1488.
4. Szafranski W, Cukier A, Ramirez A, et al. Efficacy and safety of budesonide/formoterol in the management of chronic obstructive pulmonary disease. Eur Respir J 2003; 21: 74-81.
5. Calverley PM, Boonsawat W, Cseke Z, Zhong N, Peterson S, Olsson H. Maintenance therapy with budesonide and formoterol in chronic obstructive pulmonary disease. Eur Respir J 2003; 22: 912-919.
6. Oddera S, Silvestri M, Testi R, Rossi GA. Salmeterol enhances the inhibitory activity of dexamethasone on allergen-induced blood mononuclear cell activation. Respiration 1998; 65: 199-204.
7. 2-agonists and corticosteroids on tumor necrosis factor-alpha-induced interleukin-8 release from cultured human airway smooth-muscle cells. Am J Respir Cell Mol Biol 2000; 23: 79-85.
8. Spoelstra FM, Postma DS, Hovenga H, Noordhoek JA, Kauffman HF. Additive anti-inflammatory effect of formoterol and budesonide on human lung fibroblasts. Thorax 2002; 57: 237-241.
9. Korn SH, Jerre A, Brattsand R. Effects of formoterol and budesonide on GM-CSF and IL-8 secretion by triggered human bronchial epithelial cells. Eur Respir J 2001; 17: 1070-1077.
10. 2 agonists on bronchial airway smooth muscle cells through synchronised cellular signalling. Lancet 2002; 360: 1293-1299.
11. 2-adrenergic receptor agonists in primary human lung fibroblasts and vascular smooth muscle cells. J Biol Chem 1999; 274: 1005-1010.
12. Usmani OS, Ito K, Maneechotesuwan K, et al. Glucocorticoid receptor nuclear translocation in airway cells after inhaled combination therapy. Am J Respir Crit Care Med 2005; 172: 704-712.
13. Dong Y, Aronsson M, Gustafsson JA, Okret S. The mechanism of cAMP-induced glucocorticoid receptor expression. Correlation to cellular glucocorticoid response. J Biol Chem 1989; 264: 13679-13683.
14. Webster JC, Jewell CM, Bodwell JE, Munck A, Sar M, Cidlowski JA. Mouse glucocorticoid receptor phosphorylation status influences multiple functions of the receptor protein. J Biol Chem 1997; 272: 9287-9293.
15. Orti E, Mendel DB, Smith LI, Bodwell JE, Munck A. A dynamic model of glucocorticoid receptor phosphorylation and cycling in intact cells. J Steroid Biochem 1989; 34: 85-96.
16. Doucas V, Shi Y, Miyamoto S, West A, Verma I, Evans RM. Cytoplasmic catalytic subunit of protein kinase A mediates cross-repression by NF-kB and the glucocorticoid receptor. Proc Natl Acad Sci USA 2000; 97: 11893-11898.
17. Mattoli S, Marini M, Fasoli A. Expression of the potent inflammatory cytokines, GM-CSF, IL6, and IL8, in bronchial epithelial cells of asthmatic patients. Chest 1992; 101: Suppl. 3, 27S-29S.
18. Sousa AR, Poston RN, Lane SJ, Nakhosteen JA, Lee TH. Detection of GM-CSF in asthmatic bronchial epithelium and decrease by inhaled corticosteroids. Am Rev Respir Dis 1993; 147: 1557-1561.
19. 2-adrenoceptor agonist. J Asthma 1998; 35: 401-408.
20. Korn SH, Wouters EF, Vos N, Janssen-Heininger YM. Cytokine-induced activation of nuclear factor-kappa B is inhibited by hydrogen peroxide through oxidative inactivation of IκB kinase. J Biol Chem 2001; 276: 35693-35700.
21. Blaheta RA, Franz M, Auth MK, Wenisch HJ, Markus BH. A rapid non-radioactive fluorescence assay for the measurement of both cell number and proliferation. J Immunol Methods 1991; 142: 199-206.
22. 2-agonists and corticosteroids. Eur Respir J 2002; 19: 182-191.
23. Nielsen LP, Pedersen B, Faurschou P, Madsen F, Wilcke JT, Dahl R. Salmeterol reduces the need for inhaled corticosteroid in steroid-dependent asthmatics. Respir Med 1999; 93: 863-868.
24. FitzGerald JM, Sears MR, Boulet LP, et al. Adjustable maintenance dosing with budesonide/formoterol reduces asthma exacerbations compared with traditional fixed dosing: a five-month multicentre Canadian study. Can Respir J 2003; 10: 427-434.
25. Wang JH, Trigg CJ, Devalia JL, Jordan S, Davies RJ. Effect of inhaled beclomethasone dipropionate on expression of proinflammatory cytokines and activated eosinophils in the bronchial epithelium of patients with mild asthma. J Allergy Clin Immunol 1994; 94: 1025-1034.
26. Wilson SJ, Wallin A, Della-Cioppa G, Sandstrom T, Holgate ST. Effects of budesonide and formoterol on NF-κB, adhesion molecules, and cytokines in asthma. Am J Respir Crit Care Med 2001; 164: 1047-1052.
27. Adcock IM, Caramori G. Cross-talk between pro-inflammatory transcription factors and glucocorticoids. Immunol Cell Biol 2001; 79: 376-384.
28. Park CS, Choi YS, Ki SY, et al. Granulocyte macrophage colony-stimulating factor is the main cytokine enhancing survival of eosinophils in asthmatic airways. Eur Respir J 1998; 12: 872-878.
29. 2-adrenergic receptor agonists enhance glucocorticoid actions via enhancement of glucocorticoid receptor-DNA binding in epithelial cells (BEAS2B). Am J Respir Crit Care Med 2004; 169: A848.
30. 2-adrenoceptor agonists, like glucocorticoids, repress eotaxin gene transcription by selective inhibition of histone H4 acetylation. J Immunol 2005; 175: 478-486.
31. Ito K, Yamamura S, Essilfie-Quaye S, et al. Histone deacetylase 2-mediated deacetylation of the glucocorticoid receptor enables NF-kB suppression. J Exp Med 2006; 203: 7-13.