Cytolytic and K+ channel blocking activities of β-KTx and scorpine-like peptides purified from scorpion venoms

Cellular and Molecular Life Sciences

Volumn 65, Issue 1, 2008, Pages 187-200

  Diego Garcia E ^a   Abdel Mottaleb, Y ^b   Schwartz, E F ^a,c   De La Vega, R C Rodríguez ^a   Tytgat, J ^b   Possani, L D ^a  

^a INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

^b UNIVERSITY OF LEUVEN   (Belgium)

^c UNIVERSITY OF BRASILIA   (Brazil)

Author keywords

Cysteine stabilized motif; Cytolytic peptides; DNA cloning; Gene topology; K+ channel blocker; Scorpion toxin; Sequence analysis

Indexed keywords

CYSTEINE; DISULFIDE; POTASSIUM CHANNEL BLOCKING AGENT; PROTEIN BETA KTX; PROTEIN SCORPINE; SCORPION VENOM; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANTIMICROBIAL ACTIVITY; ARTICLE; CYTOLYSIS; ELECTROPHYSIOLOGY; GENE STRUCTURE; PROTEIN ANALYSIS; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN MOTIF; PROTEIN PURIFICATION; PROTEIN STRUCTURE; SCORPION;

ANIMALS; ANTI-INFECTIVE AGENTS; CELL SURVIVAL; DEFENSINS; EVOLUTION, MOLECULAR; NEUROTOXINS; PEPTIDES; POTASSIUM CHANNEL BLOCKERS; SCORPION VENOMS;

SCORPIONES;

EID: 38549113609     PISSN: 1420682X     EISSN: 15691632     Source Type: Journal    
DOI: 10.1007/s00018-007-7370-x     Document Type: Article

References (50)

1. Possani, L. D. and Rodríguez de la Vega, R. C. (2006) Scorpion venom peptides. In: Handbook of Biologically Active Peptides, pp. 339-354, Kastin, A. J. (ed.), Academic Press, San Diego.
2. Mouhat, S., Jouirou, B., Mosbah, A., De Waard, M. and Sabatier, J. M. (2004) Diversity of folds in animal toxins acting on ion channels. Biochem. J. 378, 717-726.
3. Zhu, S., Gao, B. and Tytgat, J. (2005) Phylogenetic distribution, functional epitopes and evolution of the CSalphabeta superfamily. Cell. Mol. Life Sci. 62, 2257-2269.
4. Zeng, X. C., Corzo, G. and Hahin, R. (2005) Scorpion venom peptides without disulfide bridges. IUBMB Life 57, 13-21.
5. Luo, F., Zeng, X. C., Hahin, R., Cao, Z. J., Liu, H. and Li, W. X. (2005) Genomic organization of four novel nondisulfide-bridged peptides from scorpion Mesobuthus martensii Karsch: Gaining insight into evolutionary mechanism. Peptides 26, 2427-2433.
6. Diego-Garcia, E., Schwartz, E. F., D'Suze, G., Gonzalez, S. A., Batista, C. V., Garcia, B. I., Rodríguez de la Vega, R. C. and Possani, L. D. (2007) Wide phylogenetic distribution of Scorpine and long-chain beta-KTx-like peptides in scorpion venoms: Identification of "orphan" components. Peptides 28, 31-37.
7. + channels blocked by alpha-dendrotoxin in synaptosomes. Proc. Natl. Acad. Sci. USA 91, 1475-1479.
8. + channels. FEBS Lett. 431, 375-380.
9. Zhu, S. and Tytgat, J. (2004) The scorpine family of defensins: Gene structure, alternative polyadenylation and fold recognition. Cell. Mol. Life Sci. 61, 1751-1763.
10. Zhu, S., Li, W., Zeng, X., Jiang, D., Mao, X. and Liu, H. (1999) Molecular cloning and sequencing of two 'short chain' and two 'long chain' K(+) channel-blocking peptides from the Chinese scorpion Buthus martensii Karsch. FEBS Lett. 457, 509-514.
11. Diego-Garcia, E., Batista, C. V., Garcia-Gomez, B. I., Lucas, S., Candido, D. M., Gomez-Lagunas, F. and Possani, L. D. (2005) The Brazilian scorpion Tityus costatus Karsch: Genes, peptides and function. Toxicon 45, 273-283.
12. Batista, C. V., Roman-Gonzalez, S. A., Salas-Castillo, S. P., Zamudio, F. Z., Gomez-Lagunas, F. and Possani, L. D. (2007) Proteomic analysis of the venom from the scorpion Tityus stigmurus: Biochemical and physiological comparison with other Tityus species. Comp. Biochem. Physiol. C 146, 147-157.
13. Tytgat, J., Chandy, K. G., Garcia, M. L., Gutman, G. A., Martin-Eauclaire, M. F., van der Walt, J. J. and Possani, L. D. (1999) A unified nomenclature for short-chain peptides isolated from scorpion venoms: Alpha-KTx molecular subfamilies. Trends Pharmacol. Sci. 20, 444-447.
14. Cao, Z., Xiao, F., Peng, F., Jiang, D., Mao, X., Liu, H., Li, W., Hu, D. and Wang, T. (2003) Expression, purification and functional characterization of a recombinant scorpion venom peptide BmTXKbeta. Peptides 24, 187-192.
15. Conde, R., Zamudio, F. Z., Rodriguez, M. H. and Possani, L. D. (2000) Scorpine, an anti-malaria and anti-bacterial agent purified from scorpion venom. FEBS Lett. 471, 165-168.
16. Uawonggul, N., Thammasirirak, S., Chaveerach, A., Arkaravichien, T., Bunyatratchata, W., Ruangjirachuporn, W., Jearranaiprepame, P., Nakamura, T., Matsuda, M., Kobayashi, M., Hattori, S. and Daduang, S. (2007) Purification and characterization of Heteroscorpine-1 (HS-1) toxin from Heterometrus laoticus scorpion venom. Toxicon 49, 19-29.
17. Schwartz, E. F., Diego-Garcia, E., Rodríguez de la Vega, R. C. and Possani, L. D. (2007) Transcriptome analysis of the venom gland of the Mexican scorpion Hadrurus gertschi (Arachnida: Scorpiones). BMC Genomics 8, 119.
18. Bulet, P., Stocklin, R. and Menin, L. (2004) Anti-microbial peptides: From invertebrates to vertebrates. Immunol. Rev. 198, 169-184.
19. Cociancich, S., Goyffon, M., Bontems, F., Bulet, P., Bouet, F., Menez, A. and Hoffmann, J. A. (1993) Purification and characterization of a scorpion defensin, a 4kDa antibacterial peptide presenting structural similarities with insect defensins and scorpion toxins. Biochem. Biophys. Res. Commun. 194, 17-22.
20. Ehret-Sabatier, L., Loew, D., Goyffon, M., Fehlbaum, P., Hoffmann, J. A., van Dorsselaer, A. and Bulet, P. (1996) Characterization of novel cysteine-rich antimicrobial peptides from scorpion blood. J. Biol. Chem. 271, 29537-29544.
21. Rodríguez de la Vega, R. C., Garcia, B. I., D'Ambrosio, C., Diego-Garcia, E., Scaloni, A. and Possani, L. D. (2004) Antimicrobial peptide induction in the haemolymph of the Mexican scorpion Centruroides limpidus limpidus in response to septic injury. Cell. Mol. Life Sci. 61, 1507-1519.
22. Froy, O. and Gurevitz, M. (2004) Arthropod defensins illuminate the divergence of scorpion neurotoxins. J. Pept. Sci. 10, 714-718.
23. Rodríguez de la Vega, R. C. and Possani, L. D. (2005) On the evolution of invertebrate defensins. Trends Genet. 21, 330-332.
24. Cao, Z., Zhu, S., Li, W., Jiang, D., He, X. and Liu, H. (2001) Genomic organization of BmTXKbeta and BmTXKS2, two scorpion venom peptides from Buthus martensii Karsch. IUBMB Life 51, 305-308.
25. Zhu, S. and Gao, B. (2006) Molecular characterization of a possible progenitor sodium channel toxin from the Old World scorpion Mesobuthus martensii. FEBS Lett. 580, 5979-5987.
26. Kozminsky-Atias, A., Somech, E. and Zilberberg, N. (2007) Isolation of the first toxin from the scorpion Buthus occitanus israelis showing preference for Shaker potassium channels. FEBS Lett. 581, 2478-2484.
27. Abdel-Mottaleb, Y., Coronas, F. V., de Roodt, A. R., Possani, L. D. and Tytgat, J. (2006) A novel toxin from the venom of the scorpion Tityus trivittatus, is the first member of a new alpha-KTX subfamily. FEBS Lett. 580, 592-596.
28. +-channel specific toxin characterized from the venom of the scorpion Hadrurus gertschi Soleglad. Toxicon 48, 1046-1053.
29. Allen, G. (1981) Sequencing of Proteins and Peptides. Elsevier, Amsterdam.
30. Possani, L. D., Martin, B. M., Svendsen, I., Rode, G. S. and Erickson, B. W. (1985) Scorpion toxins from Centruroides noxius and Tityus serrulatus. Primary structures and sequence comparison by metric analysis. Biochem. J. 229, 739-750.
31. Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W. and Lipman, D. J. (1997) Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Res. 25, 3389-3402.
32. Edgar Robert. C. (2004) MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 32, 1792-1797.
33. Edgar, R. C. (2004) MUSCLE: A multiple sequence alignment method with reduced time and space complexity. BMC Bioinformatics 5, 113.
34. Eddy, S. R. (1998) Profile hidden Markov models. Bioinformatics 14, 755-763.
35. The UniProt Consortium. (2007) The Universal Protein Resource (UniProt). Nucleic Acids Res. 35, D193-D197.
36. Huelsenbeck, J. P. and Ronquist, F. (2001) MrBayes: Bayesian inference of phylogeny. Bioinformatics 17, 754-755.
37. Ronquist, F. and Huelsenbeck, J. P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572-1574.
38. Rodríguez de la Vega, R. C. (2005) A note on the evolution of spider toxins containing the ICK-motif. Toxin Rev. 24, 385-397.
39. Moerman, L., Bosteels, S., Noppe, W., Willems, J., Clynen, E., Schoofs, L., Thevissen, K., Tytgat, J., van Eldere, J., van der Walt, J. and Verdonck, F. (2002) Antibacterial and antifungal properties of alpha-helical, cationic peptides in the venom of scorpions from southern Africa. Eur. J. Biochem. 269, 4799-810.
40. National Committee for Clinical Laboratory Standards. (1999) Performance standards for antimicrobial susceptibility testing, NCCLS-approved standard M100-S9. National Committee for Clinical Laboratorial Standards, Wayne, PA.
41. Corzo, G., Escoubas, P., Villegas, E., Barnham, K. J., He, W., Norton, R. S. and Nakajima, T. (2001) Characterization of unique amphipathic antimicrobial peptides from venom of the scorpion Pandinus imperator. Biochem. J. 359, 35-45.
42. Gutman, G. A., Chandy, K. G., Grissmer, S., Lazdunski, M., McKinnon, D., Pardo, L. A., Robertson, G. A., Rudy, B., Sanguinetti, M. C., Stuhmer, W. and Wang, X. (2005) International Union of Pharmacology. LIII. Nomenclature and molecular relationships of voltage-gated potassium channels. Pharmacol. Rev. 57, 473-508.
43. Zhijian, C., Feng, L., Yingliang, W., Xin, M. and Wenxin, L. (2006) Genetic mechanisms of scorpion venom peptide diversification. Toxicon 47, 348-355.
44. Patthy, L. (2003) Modular assembly of genes and the evolution of new functions. Genetica 118, 217-231.
45. Gigangiacomo, K. M., Ceralde, Y. and Mullmann, T. J. (2004) Molecular basis of alpha-KTx specificity. Toxicon 43, 877-886.
46. + channels and scorpion toxins. Trends Pharmacol. Sci. 24, 222-227.
47. + and HERG currents. Biochem. J. 378, 745-752.
48. Vacher, H., Prestipino, G., Crest, M. and Martin-Eauclaire, M. F. (2004) Definition of the alpha-KTx15 subfamily. Toxicon 43, 887-894.
49. De Waard, M. and Sabatier, J. M. (2006) Structure-function strategies to improve the pharmacological value of animal toxins. In: Handbook of Biologically Active Peptides, pp. 415-420, Kastin, A. J. (ed.), Academic Press, San Diego.
50. Letunic, I. and Bork, P. (2007) Interactive Tree Of Life (iTOL): An online tool for phylogenetic tree display and annotation. Bioinformatics 23, 127-128.