Expression, purification and functional characterization of a recombinant scorpion venom peptide BmTXKβ

Peptides

Volumn 24, Issue 2, 2003, Pages 187-192

  Cao, Zhijian ^a   Xiao, Fan ^a   Peng, Fang ^a   Jiang, Dahe ^a   Mao, Xin ^a   Liu, Hui ^a   Li, Wenxin ^a   Hu, Dan ^b   Wang, Teng ^b  

^a WUHAN UNIVERSITY   (China)

^b RENMIN HOSPITAL OF WUHAN UNIVERSITY   (China)

Author keywords

Atrial myocyte; BmTXK ; Expression; Whole cell patch clamp

Indexed keywords

ENTEROPEPTIDASE; HYBRID PROTEIN; PEPTIDE; RECOMBINANT PROTEIN; SCORPION VENOM;

ACTION POTENTIAL; AFFINITY CHROMATOGRAPHY; AMPLITUDE MODULATION; ANIMAL CELL; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BACTERIUM CULTURE; CONCENTRATION RESPONSE; CONTROLLED STUDY; HEART ATRIUM; MUSCLE CELL; NONHUMAN; PATCH CLAMP; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RABBIT;

BACTERIA (MICROORGANISMS); BUTHUS; MESOBUTHUS MARTENSII; ORYCTOLAGUS CUNICULUS; SCORPIONES;

EID: 0037302479     PISSN: 01969781     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0196-9781(03)00025-1     Document Type: Article

References (30)

1. Amos G.J., Wettwer E., Metzger F., Li Q., Himmel H.M., Ravens V. Difference between outward currents of human atrial and subepicardial ventricular myocytes. J. Physiol. (Lond.). 491:1996;31-50.
2. Chhatwal G., Habermann E. Biochemical and pharmacological studies on the neurotoxins, protease inhibitor and histamine releaser purified from the venom of Indina red scorpion. Toxicon. 20:1982;63-73.
3. 2+]o-induced electrical heterogeneity and extrasystolic activity in isolated canine ventricular epicardium. Phase 2 reentry. Circulation. 89:1994;1839-1850.
4. Faivre J.F., Calmels T.P.G., Rouanet S., Javre J.L., Cheval B., Bril A. Characterisation of Kv4.3 in HEK293 cells: comparison with the rat ventricular transient outward potassium current. Cardiovasc. Res. 41:1999;188-199.
5. Ferreira L.A.F., Zingalli R., Habermehl G., Lebrun I. Isolation and properties of a new kallikrein inhibitor from Tityus serrulatus venom. J. Protein Chem. 17(8):1998;799-805.
6. Froy O., Gurevitz M., Froy O. Membrane potential modulators: a thread of scarlet from plants to humans. FASEB J. 12:1998;1793-1796.
7. Jiang G., Xu Y., Zhu X., Su Y., Zhu Y. Prokaryotically expressed Buthus martensii Karsch insect depressant toxin has insecticidal effects. Toxicon. 39:2001;469-476.
8. Chi-Feng H., Mei-Hwan W., Chang-Her T. Electrophysiological mechanism for the antiarrhythmic activities of naloxone on cardiac tissues. Life Sci. 63(14):1998;1205-1219.
9. Kaab S., Nuss H.B., Chiamvimonvat N., O'Rourke B., Pak P.H., Kass D.A.et al. Ionic mechanism of action potential prolongation in ventricular myocytes from dogs with pacing-induced heart failure. Circ. Res. 78(2):1996;262-273.
10. + channels. FEBS Lett. 431:1998;375-380.
11. Yang, Li. Ion channel and patch clamp. Lanzhou: Gansu literature press; 2001.
12. Nabauer M., Beuckelmann D.J., Erdmann E. Characteristics of transient outward current in human ventricular myocytes from patients with terminal heart failure. Circ. Res. 73:1993;386-394.
13. Patmore L., Fraser S., Mair D., Templeton A. Effects of sparfloxacin, grepafloxacin, moxifloxacin and ciprofloxacin on cardiac action potential duration. Eur. J. Pharm. 406:2000;449-452.
14. +-channels. Eur. J. Biochem. 264:1999;287-300.
15. Possani L.D., Merino E., Corona M., Bolivar F., Becerril B. Peptides and genes coding for scorpion toxins that affect ion-channels. Biochimie. 82:2000;861-868.
16. +-channels. Perspect. Drug Discov. Des. 15/16:1999;15-40.
17. + channels blocked by α-dendrotoxin in synaptosomes. Proc Natl Acad Sci USA 1994;91:1475-9.
18. Richard J.S., Taian C., Qiurong X., Jianlin F., Yow C.C. The production of recombinant dengue virus E protein using Escherichia coli and Pichia pastoris. J. Virol. Methods. 69:1997;159-169.
19. Rozanski G.J., Xu Z., Whitney R.T., Murakami H., Zucker I.H. Electrophysiology of rabbit ventricular myocytes following sustained rapid ventricular pacing. J. Mol. Cell Cardiol. 29(2):1997;721-732.
20. Schagger H., Gebhard V.J. Tricine-sodium dodecyl sulfate polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166:1987;368-397.
21. Srinivasan K.N., Gopalakrishnakone P., Tan P.T., Chew K.C., Cheng B., Kini R.M.et al. SCORPION, a molecular database of scorpion toxins. Toxicon. 40:2001;23-31.
22. Johnson T.M., Quick M.W., Sakai T.T., Krishna N.R. Expression of functional recombinant scorpion β-neurotoxin Css II in E. coli. Peptides. 21:2000;767-772.
23. Torres-Larios A., Gurrola G.B., Zamudio F.Z., Possani L.D. Hadrurin, a new antimicrobial peptide from the venom of the scorpion Hadrurus aztecus. Eur. J. Biochem. 267:2000;5023-5031.
24. Tytgat J., Chandy K.G., Garcia M.L., Gutman G.A., Martin-Eauclaire M.F., Possani L.D. A unified nomenclature for short-chain peptides isolated from scorpion venoms: alpha KTx molecular subfamilies. Trends Pharmacol. 20:1999;444-447.
25. Xiong Y.M., Lan Zh.D., Wang M., Liu B., Liu X.Q., Fei H.et al. Molecular characterization of a new excitatory insect neurotoxin with an analgesic effect on mice from the scorpion Buthus martensii Karsch. Toxicon. 37:1999;1165-1180.
26. + channel toxin from the Chinese scorpion Buthus martensii Karsch. Toxicon. 39:2001;407-410.
27. Cao Z.J., Zhu S.Y., Li W.X., Jiang D.H., He X.H., Liu H. Genomic organization of BmTXKβ and BmTXKS2, two scorpion venom peptides from Buthus martensii Karsch. IUBMB-Life. 51(5):2001;305-508.
28. + channel-blocking peptides from the Chinese scorpion Buthus martensii Karsch. FEBS Lett. 457:1999;509-514.
29. Zhu S.Y., Li W.X., Jiang D.H., Zeng X.C. Evidence for the existence of insect defensin-like peptide in scorpion venom. IUBMB-Life. 50:2000;1-5.
30. Zilberberg N., Gordon D., Pelhate M. Functional expression and genetic alteration of an alpha scorpion neurotoxin. Biochemistry. 35:1996;10215-10222.