Culture-independent approach of the bacterial bioaerosol diversity in the standard swine confinement buildings, and assessment of the seasonal effect

Environmental Microbiology

Volumn 10, Issue 3, 2008, Pages 665-675

  Nehme, Benjamin ^a,b,d   Létourneau, Valérie ^a,b   Forster, Robert J ^c   Veillette, Marc ^a,b   Duchaine, Caroline ^a,b  

^a LAVAL UNIVERSITY   (Canada)

^b UNIVERSITÉ LAVAL   (Canada)

^c LETHBRIDGE RESEARCH CENTRE   (Canada)

^d LAVAL UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; RIBOSOME DNA; RNA 16S;

ANIMAL; ARTICLE; BACTERIUM; BIODIVERSITY; CLASSIFICATION; GENETICS; INDOOR AIR POLLUTION; ISOLATION AND PURIFICATION; MANURE; METABOLISM; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEASON; SWINE;

AIR MICROBIOLOGY; AIR POLLUTION, INDOOR; ANIMALS; BACTERIA; BIODIVERSITY; DNA, BACTERIAL; DNA, RIBOSOMAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MANURE; MICROBIOLOGICAL TECHNIQUES; MOLECULAR SEQUENCE DATA; RNA, RIBOSOMAL, 16S; SEASONS; SWINE;

BACTERIA (MICROORGANISMS); BACTEROIDETES; CLOSTRIDIA; LACTOBACILLALES; POSIBACTERIA; SUIDAE;

EID: 38549089411     PISSN: 14622912     EISSN: 14622920     Source Type: Journal    
DOI: 10.1111/j.1462-2920.2007.01489.x     Document Type: Article

References (42)

1. Altschul, S.F., Gish, W., Miller, W., Myers, E.W. Lipman, D.J. (1990) Basic local alignment search tool. J Mol Biol 215 : 403 410.
2. Amann, R.I., Ludwig, W. Schleifer, K.H. (1995) Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev 59 : 143 169.
3. Bach, H.J., Tomanova, J., Schloter, M. Munch, J.C. (2002) Enumeration of total bacteria and bacteria with genes for proteolytic activity in pure cultures and in environmental samples by quantitative PCR mediated amplification. J Microbiol Methods 49 : 235 245.
4. Brodie, E.L., DeSantis, T.Z., Parker, J.P.M., Zubietta, I.X., Piceno, Y.M. Andersen, G.L. (2007) Urban aerosols harbor diverse and dynamic bacterial populations. PNAS 104 : 299 304.
5. Chang, C.W., Chung, H., Huang, C.F. Su, H.J. (2001) Exposure of workers to airborne microorganisms in open-air swine houses. Appl Environ Microbiol 67 : 155 161.
6. Chapin, A., Rule, A., Gibson, K., Buckley, T. Schwab, K. (2005) Airborne multidrug-resistant bacteria isolated from a concentrated swine feeding operation. Environ Health Perspect 113 : 137 142.
7. Charavaryamath, C. Singh, B. (2006) Pulmonary effects of exposure to pig barn air. J Occup Med Toxicol 1 : 10.
8. Choudat, D., Goehen, M., Korobaeff, M., Boulet, A., Dewitte, J.D. Martin, M.H. (1994) Respiratory symptoms and bronchial reactivity among pig and dairy farmers. Scand J Work Environ Health 20 : 48 54.
9. Cole, J.R., Chai, B., Marsh, T.L., Farris, R.J., Wang, Q., Kulam, S.A., et al. (2003) The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res 31 : 442 443.
10. Collins, M.D., Lawson, P.A., Willems, A., Cordoba, J.J., Fernandez-Garayzabal, J., Garcia, P., et al. (1994) The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44 : 812 826.
11. Cormier, Y., Tremblay, G., Meriaux, A., Brochu, G. Lavoie, J. (1990) Airborne microbial contents in two types of swine confinement buildings in Quebec. Am Ind Hyg Assoc J 51 : 304 309.
12. Cormier, Y., Israel-Assayag, E., Racine, G. Duchaine, C. (2000) Farming practices and the respiratory health risks of swine confinement buildings. Eur Respir J 15 : 560 565.
13. Crook, B., Robertson, J.F., Glass, S.A., Botheroyd, E.M., Lacey, J. Topping, M.D. (1991) Airborne dust, ammonia, microorganisms, and antigens in pig confinement houses and the respiratory health of exposed farm workers. Am Ind Hyg Assoc J 52 : 271 279.
14. Donham, K., Haglind, P., Peterson, Y., Rylander, R. Belin, L. (1989) Environmental and health studies of farm workers in Swedish swine confinement buildings. Br J Ind Med 46 : 31 37.
15. Donham, K.J. (1991) Association of environmental air contaminants with disease and productivity in swine. Am J Vet Res 52 : 1723 1730.
16. Donham, K.J., Popendorf, W., Palmgren, U. Larsson, L. (1986) Characterization of dusts collected from swine confinement buildings. Am J Ind Med 10 : 294 297.
17. Dosman, J.A., Lawson, J.A., Kirychuk, S.P., Cormier, Y., Biem, J. Koehncke, N. (2004) Occupational asthma in newly employed workers in intensive swine confinement facilities. Eur Respir J 24 : 698 702.
18. Duchaine, C., Grimard, Y. Cormier, Y. (2000) Influence of building maintenance, environmental factors, and seasons on airborne contaminants of swine confinement buildings. AIHAJ 61 : 56 63.
19. Dutil, S., Veillette, M., Meriaux, A., Lazure, L., Barbeau, J. Duchaine, C. (2007) Aerosolization of mycobacteria and legionellae during dental treatment: low exposure despite dental unit contamination. Environ Microbiol 9 : 2836 2843.
20. Galtier, N., Gouy, M. Gautier, C. (1996) seaview and phylo_win: two graphic tools for sequence alignment and molecular phylogeny. Comput Appl Biosci 12 : 543 548.
21. Gibbs, S.G., Green, C.F., Tarwater, P.M. Scarpino, P.V. (2004) Airborne antibiotic resistant and nonresistant bacteria and fungi recovered from two swine herd confined animal feeding operations. J Occup Environ Hyg 1 : 699 706.
22. Gibbs, S.G., Green, C.F., Tarwater, P.M., Mota, L.C., Mena, K.D. Scarpino, P.V. (2006) Isolation of antibiotic-resistant bacteria from the air plume downwind of a swine confined or concentrated animal feeding operation. Environ Health Perspect 114 : 1032 1037.
23. Gill, S.R., Pop, M., Deboy, R.T., Eckburg, P.B., Turnbaugh, P.J., Samuel, B.S., et al. (2006) Metagenomic analysis of the human distal gut microbiome. Science 312 : 1355 1359.
24. Gorny, R.L., Reponen, T., Willeke, K., Schmechel, D., Robine, E., Boissier, M. Grinshpun, S.A. (2002) Fungal fragments as indoor air biocontaminants. Appl Environ Microbiol 68 : 3522 3531.
25. Heederik, D., Brouwer, R., Biersteker, K. Boleij, J.S. (1991) Relationship of airborne endotoxin and bacteria levels in pig farms with the lung function and respiratory symptoms of farmers. Int Arch Occup Environ Health 62 : 595 601.
26. Israel-Assayag, E. Cormier, Y. (2002) Adaptation to organic dust exposure: a potential role of 1-selectin shedding? Eur Respir J 19 : 833 837.
27. Klappenbach, J.A., Saxman, P.R., Cole, J.R. Schmidt, T.M. (2001) rrndb: the Ribosomal RNA Operon Copy Number Database. Nucleic Acids Res 29 : 181 184.
28. Leser, T.D., Amenuvor, J.Z., Jensen, T.K., Lindecrona, R.H., Boye, M. Moller, K. (2002) Culture-independent analysis of gut bacteria: the pig gastrointestinal tract microbiota revisited. Appl Environ Microbiol 68 : 673 690.
29. Leung, K. Topp, E. (2001) Bacterial community dynamics in liquid swine manure during storage: molecular analysis using DGGE/PCR of 16S rDNA. FEMS Microbiol Ecol 38 : 169 177.
30. Ley, R.E., Backhed, F., Turnbaugh, P., Lozupone, C.A., Knight, R.D. Gordon, J.I. (2005) Obesity alters gut microbial ecology. Proc Natl Acad Sci USA 102 : 11070 11075.
31. Mackiewicz, B. (1998) Study on exposure of pig farm workers to bioaerosols, immunologic reactivity and health effects. Ann Agric Environ Med 5 : 169 175.
32. Marchesi, J.R., Sato, T., Weightman, A.J., Martin, T.A., Fry, J.C., Hiom, S.J., et al. (1998) Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl Environ Microbiol 64 : 795 799.
33. Muyzer, G., de Waal, E.C. Uitterlinden, A.G. (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59 : 695 700.
34. Predicala, B.Z., Urban, J.E., Maghirang, R.G., Jerez, S.B. Goodband, R.D. (2002) Assessment of bioaerosols in swine barns by filtration and impaction. Curr Microbiol 44 : 136 140.
35. Robbins, C.A., Swenson, L.J., Nealley, M.L., Gots, R.E. Kelman, B.J. (2000) Health effects of mycotoxins in indoor air: a critical review. Appl Occup Environ Hyg 15 : 773 784.
36. Schmalenberger, A., Schwieger, F. Tebbe, C.C. (2001) Effect of primers hybridizing to different evolutionarily conserved regions of the small-subunit rRNA gene in PCR-based microbial community analyses and genetic profiling. Appl Environ Microbiol 67 : 3557 3563.
37. Snell-Castro, R., Godon, J.J., Delgenes, J.P. Dabert, P. (2005) Characterisation of the microbial diversity in a pig manure storage pit using small subunit rDNA sequence analysis. FEMS Microbiol Ecol 52 : 229 242.
38. Thompson, J.D., Higgins, D.G. Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22 : 4673 4680.
39. Wang, G.C. Wang, Y. (1996) The frequency of chimeric molecules as a consequence of PCR co-amplification of 16S rRNA genes from different bacterial species. Microbiology 142 : 1107 1114.
40. Wang, G.C. Wang, Y. (1997) Frequency of formation of chimeric molecules as a consequence of PCR coamplification of 16S rRNA genes from mixed bacterial genomes. Appl Environ Microbiol 63 : 4645 4650.
41. Whitford, M.F., Forster, R.J., Beard, C.E., Gong, J. Teather, R.M. (1998) Phylogenetic Analysis of Rumen Bacteria by Comparative Sequence Analysis of Cloned 16S rRNA Genes[ss]. Anaerobe 4 : 153 163.
42. Wilson, K.H. Blitchington, R.B. (1996) Human colonic biota studied by ribosomal DNA sequence analysis. Appl Environ Microbiol 62 : 2273 2278.