A food-grade delivery system for Lactococcus lactis and evaluation of inducible gene expression

Applied Microbiology and Biotechnology

Volumn 65, Issue 1, 2004, Pages 61-67

  Simoes Barbosa A ^a   Abreu, H ^a   Silva Neto, A ^a   Gruss, A ^b   Langella, P ^b  

^a UNIVERSIDADE CATÓLICA DE BRASÍLIA   (Brazil)

^b CEMAGREF   (France)

Author keywords

[No Author keywords available]

Indexed keywords

HISTIDINE; NISIN; NUCLEASE;

BACTERIUM; BIOTECHNOLOGY;

AMINO ACID SYNTHESIS; ARTICLE; BACTERIUM MUTANT; CELL TRANSFORMATION; CONTROLLED STUDY; EPISOME; FOOD INDUSTRY; GENE EXPRESSION; GENE INDUCTION; GENE VECTOR; GENOTYPE; HOMOLOGOUS RECOMBINATION; LACTOCOCCUS LACTIS; NONHUMAN; PHENOTYPE; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN INDUCTION; REPORTER GENE; STAPHYLOCOCCUS AUREUS;

BACTERIAL PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; LACTOCOCCUS LACTIS; NISIN; PLASMIDS; RECOMBINANT FUSION PROTEINS;

LACTOCOCCUS; LACTOCOCCUS LACTIS; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS;

EID: 3843124236     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (43)

1. Asseldonk M van, Rutten G, Oteman M, Siezen RJ, Vos WM de, Simons G (1990) Cloning of usp45, a gene encoding a secreted protein from Lactococcus lactis subs. lactis MG1363. Gene 95:155-160
2. Asseldonk M van, Vos WM de, Simons G (1993) Functional analysis of the Lactococcus lactis usp45 secretion signal in the secretion of a homologous proteinase and a heterologous α-amylase. MoI Gen Genet 240:428-434
3. Bron PA, Benchimol MG, Lambert J, Palumbo E, Deghorain M, Delcour J, Vos WM de, Kleerebezem M Hols P (2002) Use of the air gene as a food-grade selection marker in lactic acid bacteria. Appl Environ Microbiol 68:5663-5670
4. Cuatrecasas P, Fuchs S, Anfisen CB (1967) Catalytic properties and specificity of the extracellular nuclease of Staphylococcus aureus. J Biol Chem 242:1541-1547
5. Davis A, Moore IB, Parker DS, Taniuchi H (1977) Nuclease B: a possible precursor of nuclease A, an extracellular nuclease of Staphylococcus aureus. J Biol Chem 252:6544-6553
6. Delorme C, Ehrlich SD, Renault P(1992) Histidine biosynthesis genes in Lactococcus lactis subsp. lactis. J Bacteriol 174:6571-6579
7. Dickley F, Nilsson D, Hansen EB, Johansen E (1995) Isolation of Lactococcus lactis nonsense suppressors and construction of a food-grade cloning vector. Mol Microbiol 15:839-847
8. Djordjevic GM, Klaenhammer TR (1998) Inducible gene expression systems in Lactococcus lactis. Mol Biotechnol 9:127-139
9. Eichenbaum Z, Federle MJ, Marra D, Vos WM de, Kuipers OP, Kleerebezem M, Scott JR (1998) Use of the lactococcal nisA promoter to regulate gene expression in gram-positive bacteria: comparison of induction level and promoter strength. Appl Environ Microbiol 64:2763-2769
10. Emond E, Lavallee R, Drolet G, Moineau S, LaPointe G (2001) Molecular characterization of a theta replication plasmid and its use for development of a two-component food-grade cloning system for Lactococcus lactis. Appl Environ Microbiol 67:1700-1709
11. Enouf V, Langella P, Commissaire J, Cohen J, Corthier G (2001) Bovine rotavirus nonstructural protein 4 produced by Lactococcus lactis is antigenic and immunogenic. Appl Environ Microbiol 67:1423-1428
12. Federal Register (1988) Nisin preparation: affirmation of GRAS status as a direct human food ingredient. Fed Regist 54:11247-11251
13. Gory L, Montel MC, Zagorec M (2001) Use of green fluorescent protein to monitor Lactobacillus sakei in fermented meat products. FEMS Microbiol Lett 194:127-133
14. Henrich B, Klein JR, Weber B, Delorme C, Renault P, Wegmann U (2002) Food-grade delivery system for controlled gene expression in Lactococcus lactis. Appl Environ Microbiol 68:5429-5436
15. Kleerebezem M, Beerthuyzen MM, Vaughan EE, Vos WM de, Kuipers OP (1997) Controlled gene expression systems for lactic acid bacteria: transferable nisin-inducible expression cassettes for Lactococcus, Leuconostoc, and Lactobacillus spp. Appl Environ Microbiol 63:4581-4584
16. Kovacevic S, Veal LE, Hsiung HM, Miller JR (1985) Secretion of staphylococcal nuclease by Bacillus subtilis. J Bacteriol 162:521-528
17. Kuipers OP, Beerthuyzen MM, Ruyter PG de, Luesink EJ, Vos WM de (1995) Autoregulation of nisin biosynthesis in Lactococcus lactis by signal transduction. J Biol Chem 270:27299-27304
18. Kuipers OP, Ruyter PG de, Kleerebezem M, Vos WM de (1997) Controlled overproduction of proteins by lactic acid bacteria. Trends Biotechnol 15:135-140
19. Langella P, Le Loir Y (1999) Heterologous protein secretion in Lactococcus lactis: a novel antigen delivery system. Braz J Med Biol Res 32:191-198
20. Langella P, Le Loir Y, Ehrlich SD, Gruss A (1993) Efficient plasmid mobilization by pIP501 in Lactococcus lactis subsp. lactis. J Bacteriol 175:5806-5813
21. Le Loir Y, Gruss A, Ehrlich SD, Langella P (1994) Direct screening of recombinants in Gram-positive bacteria using the secreted staphylococcal nuclease as a reporter. J Bacteriol 176:5135-5139
22. Le Loir Y, Gruss A, Ehrlich SD, Langella P (1998) A nine-residue synthetic propeptide enhances secretion efficiency of heterologous proteins in Lactococcus lactis. J Bacteriol 180:1895-1903
23. Le Loir Y, Nouaille S, Ribeiro L, Comissaire J, Cortheier G, Gilbert S, Chatel J, L'Haridon R, Gruss A, Langella P (2001) Sécrétion de protéines d'intérêt thérapeutique chez Lactococcus lactis. Lait 81:217-226
24. Leenhouts K, Buist G, Bolhuis A, Berge A ten, Kiel J, Mierau I, Dabrowska M, Venema G, Kok J (1996) A general system for generating unlabelled gene replacements in bacterial chromosomes. Mol Gen Genet 253:217-224
25. Maguin E, Prevost H, Ehrlich SD, Gruss A (1996) Efficient insertional mutagenesis in lactococci and other Gram-positive bacteria. J Bacteriol 178:931-935
26. Meer JR van der, Polman J, Beerthuyzen MM, Siezen RJ, Kuipers OP, Vos WM de (1993) Characterization of the Lactococcus lactis nisin A operon genes nisP, encoding a subtilisin-like serine protease involved in precursor processing, and nisR, encoding a regulatory protein involved in nisin biosynthesis. J Bacteriol 175:2578-2588
27. Piard JC, Hautefort I, Fischetti VA, Ehrlich SD, Fons M, Gruss A (1997) Cell-wall anchoring of the Streptococcus pyogenes M6 protein in various lactic acid bacteria. J Bacteriol 179:3068-3072
28. Platteeuw C, Alen-Boerrigter I van, Shalkwijk S van, Vos WM de (1996) Food-grade cloning and expression system for Lactococcus lactis. Appl Environ Microbiol 62:1008-1013
29. Poquet I, Ehrlich SD, Gruss A (1997) An export-specific reporter designed for gram-positive bacteria: application to Lactococcus lactis. J Bacteriol 180:1904-1912
30. Ravn P, Arnay J, Madsen MS, Vrang A, Israelsen H (2003) Optimization of signal peptide SP310 for heterologous protein production in Lactococcus lactis. Microbiology 149:2193-2201
31. Ribeiro LA, Azevedo V, Le Loir Y, Oliveira SC, Dieye Y, Piard JC, Gruss A, Langella P (2002) Production and targeting of the Brucella abortus antigen L7/L12 in Lactococcus lactis: a first step towards food-grade live vaccines against brucellosis. Appl Environ Microbiol 68:910-916
32. Rooijen RJ van, Gasson MJ, Vos WM de (1992) Characterization of the Lactococcus lactis lactose operon promoter: contribution of flanking sequences and LacR repressor to promoter activity. J Bacteriol 174:2273-2280
33. Ruiter PG de, Kuipers OP, Vos WM de (1996) Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisin. Appl Environ Microbiol 62:3662-3667
34. Sambrook J, Fritish EF, Maniatis T (2001) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
35. Shortle D (1983) A genetic system for analysis of staphylococcal nuclease. Gene 22:181-189
36. Simon D, Chopin A (1988) Construction of a vector plasmid family and its use for molecular cloning in Streptococcus lactis. Biochemie 70:559-566
37. Soresen KI, Larsen R, Kibenich A, Junge MP, Johansen E (2000) A food-grade cloning system for industrial strains of Lactococcus lactis. Appl Environ Microbiol 66:1253-1258
38. Steidler L, Robinson K, Chamberlain L, Schofield KM, Remaut E, Le Page RW, Wells JM (1998) Mucosal delivery of murine interleukin-2 (IL-2) and IL-6 by recombinant strains of Lactococcus lactis coexpressing antigen and cytokine. Infect Immun 66:3183-3189
39. Takala TM, Saris PE (2002) A food-grade cloning vector for lactic acid bacteria based on the nisin immunity gene nisl. Appl Microbiol Biotechnol 59:467-471
40. Vos WM de (1999a) Safe and sustainable systems for food-grade fermentations by genetically modified lactic acid bacteria. Int Dairy J 9:3-10
41. Vos WM de (1999b) Gene expression systems for lactic acid bacteria. Curr Opin Microbiol 2:289-295
42. Vos WM de, Simons GFM (1994) Gene cloning and expression in lactococci. In: Gasson MJ, Vos WM de (eds) Genetics and biotechnology of lactic acid bacteria. Chapman and Hall, London, pp 52-105
43. Vossen JM van der, Lelie D van der, Venema G (1987) Isolation and characterization of Streptococcus cremoris Wg2-specific promoters. Appl Environ Microbiol 53:2452-2457