Renaturation, purification and characterization of streptokinase expressed as inclusion body in recombinant E. coli

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 861, Issue 2, 2008, Pages 218-226

  Cherish Babu, P V ^a   Srinivas, V K ^a   Krishna Mohan, V ^a   Krishna, Ella ^a  

^a Bharat Biotech International Limited   (India)

Author keywords

Escherichia coli fermentation; Inclusion body isolation; Physiochemical characterization; Protein purification; Recombinant streptokinase (SK); Solubilization

Indexed keywords

ADDITIVES; CONFORMATIONS; ESCHERICHIA COLI; GENE EXPRESSION; PURIFICATION; SIZE EXCLUSION CHROMATOGRAPHY; SOLUBILITY;

INCLUSION BODY ISOLATION; PHYSIOCHEMICAL CHARACTERIZATION; RECOMBINANT STREPTOKINASE; SOLUBILIZATION;

PROTEINS;

STREPTOKINASE;

ARTICLE; CELL INCLUSION; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME DENATURATION; ENZYME PURIFICATION; ENZYME RENATURATION; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SOLUBILIZATION;

CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, ION EXCHANGE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; INCLUSION BODIES; PROTEIN FOLDING; PROTEIN RENATURATION; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; STREPTOKINASE;

ESCHERICHIA COLI;

EID: 38349138998     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2007.10.008     Document Type: Article

References (25)

1. Tillet W.S., and Garner R.I. J. Exp. Med. 58 (1933) 485
2. L.I. Feldman, United State Patent No. 3,855,065 (1974).
3. Jackson K.W., Malke H., Gerlach D., Ferretti J.J., and Tang T. Biochemistry 25 1 (1986) 108
4. Mason D.T. Am. Heart. J. 102 (1981) 1123
5. Marder V.J., Soulen R.L., Atichartakarn V., Budzynski A.Z., Parulekar S., Kim J.R., Edward N., Zahavi J., and Algazy K.M. J. Lab. Clin. Med. 89 5 (1977) 1018
6. Martin M. Pro. Cardiovasc. Dis. 21 (1974) 351
7. Kohner E.M., Pettit J.E., Hamilton A.M., Bulpitt C.J., and Dollery C.T. Br. Med. J. 1 (1976) 550
8. Christensen L.R. J. Clin. Invest. 28 (1949) 163
9. Reddy K.N.N., and Markus G. J. Biol. Chem. 247 6 (1972) 1683
10. Muller J., Reinert H., and Malke H. J. Bacteriol. 171 (1989) 2202
11. Malke H., and Ferretti J.J. Proc. Natl. Acad. Sci. 81 11 (1984) 3557
12. Malke H., Bruce R., and Ferretti J.J. Gene 34 (1985) 357
13. Wu X.C., Ye R.Q., Duan Y.J., and Wong S.L. Appl. Environ. Microbiol. 64 (1998) 824
14. Malke H., Gerlach D., Kohler W., and Ferretti J.J. Mol. Gen. Genet. 196 (1984) 360
15. Estrada M.P., Hernandez L., Perez A., Roderiguez P., Serrano R., Rubiera R., Pedraza A., Padron G., Antuch W., de la Fuente J., and Herrera L. Biotechnology 10 (1992) 1138
16. Avilan L., Yarzabal A., Jurgensen J., Bastidas M., Cruz J., and Puig J. Braz. J. Med. Biol. Res. 30 (1997) 1427
17. Pupo E., Baghbaderani B.A., Lugo V., Fernandez J., Paez R., and Torrens I. Biotechnol. Lett. 21 (1999) 1119
18. Lowry O.H., Rosebrough N.J., Farr A.L., and Randall R.J. J. Biol. Chem. 193 (1951) 265
19. De Renzo E.C., Siiteri P.K., Hutchings B.L., and Bell P.H. J. Biol. Chem. 242 3 (1967) 533
20. Perez N., Urrutia E., Camino J., Orta D.R., Torres Y., Martinez Y., Cruz M., Alburquerque S., Gil M.R., and Hernandez L. Minerva Biotechnol. 10 (1998) 174
21. Zhang X.W., Sun T., Huang X.N., Liu X., Gu D.X., and Tang Z.Q. Enzyme Microb. Technol. 24 (1999) 647
22. Yazdani S.S., and Mukherjee K.J. Bioprocess. Biosyst. Eng. 24 (2002) 341
23. Vallejo L.F., and Rinas U. Biotechnol. Bioeng. 85 (2004) 601
24. Robert R., and Thomas H. Arch. Biochem. Biophys. 211 (1981) 750
25. Karas M., and Hillenkamp F. Anal. Chem. 60 (1988) 2299